Analisis Sekuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba di Indonesia

Haryuningtyas, Dyah; Artama, Wayan Tunas

doi:10.21082/jbio.v4n2.2008.p45-50

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Penghilangan satu atau dua alel pada populasi yang peka terhadap benzimidazole mengindikasikan terjadi seleksi resistensi. Alel yang hilang mengubah kepekaan terhadap obat (Haryuningtyas 2005). Berdasarkan hasil identifikasi larva dan tingginya persentase pertumbuhan larva Haemonchus spp, dapat dinyatakan bahwa Haemonchus spp mengalami resistensi terhadap anthelmintik albendazole.…”

Section: Menyatakan Bahwa Populasiunclassified

Deteksi Larva Cacing Nematoda Sebelum dan Sesudah Pengobatan pada Beberapa Bangsa Domba

Dewi

Martindah

Sawitri

et al. 2017

Teknologi Peternakan Dan Veteriner Mendukung Diversifikasi Sumber Protein Asal Ternak

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Gastrointestinal nematode infection is one of the factors inhibiting the productivity of sheep. The purpose of this study was to determine the type of larvae and the percentage growth of the nematode are possible develop anthelmintic resistance in sheep. Feces from seven breeds on a farm sheep in Balitnak Bogor were collected. Each breed was taken 20 heads for samples. Sheep were treated with albendazole (benzimidazoles group). Faecal samples were taken on days 0 (before treatment), day seven and day 14 post-treatment. The number of eggs in stool were examined (egg per gram) as a method of Fecal Egg Count Reduction Test (FECRT) and cultured to determine the growth of larvae of nematodes existing types. The percentage of larvae growing were calculated and the larval nematodes are identified. Percentage growth of larvae and larval nematode species identification is an advanced stage of FECRT. The nematode larvae identified were Haemonchus spp, Trichostrongylus spp, Cooperia spp and Oesophagostomum spp. Haemonhus is larvae that grow the most in every breeds of sheep both from a stool sample day 0 (before treatment) and day 7 and 14 post-treatment. Haemonchus did not undergo inhibition in its development, although the sheep had been given treatment. Based on identification of larvae and larval growth, Haemonchus has high percentage and it can be stated that Haemonchus develop resistance to albendazole anthelmentic (benzimidazoles group). The high percentage of larvae of Haemonchus compared to other worm larvae can harm livestock productivity, therefore it is needed appropriate control strategies. Good livestock management, epidemiological studies, and the possible use of molecular technology could be used control of parasitic diseases

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Section: Menyatakan Bahwa Populasiunclassified

Deteksi Larva Cacing Nematoda Sebelum dan Sesudah Pengobatan pada Beberapa Bangsa Domba

Dewi

Martindah

Sawitri

et al. 2017

Teknologi Peternakan Dan Veteriner Mendukung Diversifikasi Sumber Protein Asal Ternak

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“…Sheep and goats are the most common small ruminants raised by Indonesian farmers. Infestation with gastrointestinal nematode worms is one of the most significant diseases that reduce sheep productivity and cause the death of young livestock [1]. These animals are susceptible to the pathogens Hemonchus contortus, Trichostrongylus spp, Oesophagostomum sp, Cooperia sp, Tricuris sp, and Bunostomum sp [2][3][4], which are reported to generate significant economic losses to ruminants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Gamal Leaves (Gliricidia Sepium) as Anthelmintic Forages Against Gastrointestinal Nematodes in Sheep

Sawitri¹,

Wardhana²,

Nefho³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Several studies have demonstrated that ruminant-fed forages containing bioactive anthelmintics (tannins, alkaloids, and flavonoids) can be used to suppress nematode worms. The objective of this study was to examine any potential direct anthelmintic effects of G.sepium leaves containing Plant Secondary Metabolites (PSM) on the Gastrointestinal Nematode (GIN) in sheep. Twentyfive adult female and male sheep that were naturally infected with nematodes were used in this study. The experimental design utilised a randomised layout with five treatment groups (K1-K5). Each group was composed of five sheep. K1 and K2 received 30% and 50% of their forage feed from G.sepium leaves, respectively, while K3 received 60 g of G.sepium leave powder. Group K4 was a positive control getting the recommended dose of albendazole, and K5 was a negative control (no treatment). Gliricidia sepium leaves and flour was administered every day for 21 days (3 weeks). Evaluation of the anthelmintic effect was conducted using the Fecal Egg Count Reduction Test (FECRT) once a week for 56 days (8 weeks), Packed Cell Volume (PCV) values and faecal culture to detect the dominating growth of nematode larvae types. The results showed that the administration of withered fresh gamal leaves as much as 30% and 50% forages decreased eggs per gram faeces (EPG) in the first until the third week of therapy with faecal egg count reduction of 61-63% and 47-56%, respectively. The effectiveness of G.sepium flour at 60 g/head/day ranged from 26 to 39%. The effectiveness of all group treatments increased after the fourth week of treatment cessation. From the first week of observation until the end of the trial, the EPG of the negative control increased by 30 to > 100%. Most of the sheep showed normal PCV values. The dominant nematode larvae identified were Haemonchus contortus (83.10%),Trichostrongylus sp(15.8%),Oesophagostomum sp (0.83%) and Cooperia sp (0.27%). Haemonchus contortus larvae pose the greatest threat to sheep productivity; consequently, effective control measures are required. These results are highly valuable for farmers, particularly in breeding groups with high helminthiasis prevalence that is challenging to treat with anthelmintics because the majority of ewes are pregnant. The combination of the utilised anthelmintic and Gliricidia sepium leaves will produce the best results in preventing nematodiasis.

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“…The use of natural anti-parasitic compounds is considered an appropriate treatment to control parasites. There is concern that the use of commercial anthelmintic drugs such as albendazole and avermectin, which have a wide antiparasitic spectrum will lead to resistant parasites because of the inefficiency of the treatment itself (Taylor et al, 2016;Haryuningtyas, 2008;Gilleard, 2006). Tannins have beneficial effects to reduce cell mucosa and permeability, and act as an anti-parasitic compound in the livestock gut (Paolini et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Calliandra calothyrsus and Artocarpus heterophyllus as anti-parasite for Bligon Goat

Setyono

Kustantinah

Indarto

et al. 2019

J. Indonesian Trop. Anim. Agric.

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This study investigated the nutritional potency and efficacy of tannin-containing forages as anti-parasites sources to support the performance of female Bligon goats. Fifteen female Bligon goats were divided into 3 different groups based on feeding treatments. The first group (KG) served as the control and was fed 100% king grass (dry matter basis). The second group (KGC) was fed 50% king grass and 50% Calliandra calothyrsus leaves (dry matter basis). The third group (KGA) was fed 50% king grass and 50% Artocarpus heterophyllus leaves (dry matter basis). The study showed that supplementation with tannin-containing forages could improve feed consumption and the digestibility of dry matter (DM), organic matter (OM), and crude protein (CP). The groups that received tannin-containing forages had higher body weight gains at 22.85 g/day and 29.52 g/day for KGC and KGA respectively than the control group. The anti-parasites effects were successfully indicated by the declining number of coccidian oocysts in the feces (number/gram feces), which were 3,166; 841; and 450 for KG, KGC, and KGA respectively. Tannin-containing forage supplementation could improve the consumption and digestibility of DM, OM and CP. Supplementation improved the daily body weight gain. The number of coccidian oocysts in animals receiving supplementation was also reduced. The greatest effects on fecal coccidian oocyst reduction were attained with Jackfruit leaves (Artocarpus heterophyllus) supplementation

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Analisis Sekuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba di Indonesia

Cited by 4 publications

References 12 publications

Deteksi Larva Cacing Nematoda Sebelum dan Sesudah Pengobatan pada Beberapa Bangsa Domba

Deteksi Larva Cacing Nematoda Sebelum dan Sesudah Pengobatan pada Beberapa Bangsa Domba

Evaluation of Gamal Leaves (Gliricidia Sepium) as Anthelmintic Forages Against Gastrointestinal Nematodes in Sheep

Calliandra calothyrsus and Artocarpus heterophyllus as anti-parasite for Bligon Goat

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