Analisis In Silico Aktivitas antimikroba Peptida dari Susu Kuda Sumbawa

Abstract

Microorganisms that are resistant to various drugs remains a serious problem for human and livestock around the world when the conventional drugs loss their activity against to the microbial target. Antimicrobial peptide represents a promising alternative to combat the pathogens. The aims of this study was to evaluate the antimicrobial activity of the peptides derived from Sumbawa mare milk protein using in silico analysis. Peptide LVNELTEFAK, HPYFYAPELLYYANK and LANSTEFAK were analyzed in silico using softwares to predict their potency as antimicrobia. The result of in silico analysis showed that different software produce various prediction of antimicrobial activity. Most of software used predicted that peptide LVNELTEFAK, HPYFYAPELLYYANK and LANSTEFAK were not categorized as antimicrobial peptides. Although those peptides showed higher therapeutic index prediction than Cecropin A but they showed less inhibition activities against Escherichia coli and Candida albicans. It was concluded that more than one software was needed to obtained more accurate prediction. This result is able to use as basic for screening, designing and finding new antimicrobia.