Análise morfométrica do endotélio corneano de coelhos à microscopia

Pigatto, João Antônio Tadeu; Abib, Fernando César; Pizzeti, Julio Cesar; Laus, José Luiz; Santos, Jaime Maia dos; Barros, Paulo Sérgio de Moraes

doi:10.22456/1679-9216.14441

Cited by 7 publications

(11 citation statements)

References 12 publications

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“…Among the limitations of specular microscopy, difficulties in obtaining images in corneas with oedema and in capturing images of peripheral regions of the cornea have been mentioned [19]. When the cornea is not transparent, in vitro techniques have been employed for endothelial analysis, achieving good results [11][12][13]16,24]. In the present study, coloration and optical microscopy enabled the analysis, photography and the study of the shape of the endothelium in all regions of the cornea.…”

Section: Discussionmentioning

confidence: 83%

“…Several techniques are available for the analysis of corneal endothelium, including mainly SEM, specular microscopy and optical microscopy [4,11,12,20]. Currently, specular microscopy is the technique most used for clinical evaluations in humans and animals and for the analysis of surgical risks, mainly in cataract surgery [1][2][3]6,14,15].…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of the cellular morphology or the percentage of hexagonal cells is among the main parameters used to evaluate the health of the corneal endothelium [4,[11][12][13]15]. At present, there is no consensus regarding the minimum number of cells that should be analysed to obtain maximum reliability in the analysed sample.…”

Section: Discussionmentioning

confidence: 99%

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Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Clerot

Hünning

Bettio

et al. 2019

Acta Scientiae. Vet.

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Background:The corneal endothelium is a monolayer of polygonal cells which constitute the last layer of the cornea. The integrity of this layer is critical to cornea transparency. The characterization of normal corneal endothelial morphology is important not only to clinical evaluation but also to selection of areas of the cornea with better quality to be employed as donor tissue. The aim of the present study was to evaluate the morphology of endothelial cells from different regions of the swine cornea after alizarin red staining using optical microscopy. Materials, Methods & Results:Twenty-four healthy eyes from 12 swine Large White breed, with 14-monthold, males or females obtained from a licensed Brazilian commercial slaughterhouse were studied. Immediately after humane slaughter, the eyes were enucleated and submitted to ophthalmic examination. Eyes with signs of diseases of the anterior segment were excluded. The cornea, with 3 mm of the sclera, was removed and placed on a glass microscope slide with the endothelial side up. Four radial incisions were made in the periphery of the cornea to better accommodate the cornea on the microscope slide. Alizarin red was diluted in isotonic solution (0.2 g/100 mL) and the pH was adjusted to 4.2 with hydrochloric acid. Three drops of alizarin red were placed on the corneal endothelium. After 90 s, the dye was removed from the cornea with balanced saline solution. The corneal endothelium was examined and photographed using an optical microscope. All evaluations were performed by the same investigator. Photomicrographs were taken of central, superior, inferior, nasal and temporal corneal areas. Parameter studied included endothelial cell morphology. For the statistical analysis, was employed the ANOVA variance test (repeated measures). Differences were considered statistically significant at P < 0.05. Normal endothelium cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. There were no significant statistical differences in the proportion of the morphology and the different regions of the cornea (P = 0.31). Discussion: Different techniques are available for the analysis of corneal endothelium, including mainly scanning electron microscopy, specular microscopy and optical microscopy. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and to obtain images of the swine endothelium of all regions of the cornea. The analysis of the cellular morphology or the percentage of hexagonal cells are among the main parameters used to evaluate the health of the corneal endothelium. In this study, the endothelium had the predominance of the hexagonal shape in all regions studied. In swine, there are no studies...

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Clerot

Hünning

Bettio

et al. 2019

Acta Scientiae. Vet.

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“…Brilliant blue was tested for cromovitrectomy and phacoemulsification, as a facilitator of capsulorhexis for improving anterior lens capsule visualization (Hisatomi et al, 2006). Currently, specular microscopy, vital staining and scanning electron microscopy (SEM) are used to evaluate the corneal endothelium (Chang et al, 2005;Pigatto et al, 2005a;Pigatto et al, 2005b;Pigatto et al, 2006;Pigatto et al, 2009;Landry et al, 2011;Bercht et al, 2015;Faganello et al, 2016). Light microscopy has been used to evaluate corneal endothelial toxicity due to different medications (Trivedi et al, 2003;Saad et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The best visualization of endothelial cells is achieved with the aid of alizarin red that stains cellular borders and trypan blue that stains the nucleus of disrupted cells (Taylor & Hunt, 1981). Scanning electron microscopy has been widely used to evaluate and compare the ultrastructure of endothelial cells of vertebrates and to evaluate the effect of drugs and surgery on corneal endothelium (Doughty et al, 1997;Pigatto et al, 2005a;Pigatto et al, 2005b;Pigatto et al, 2009). The use of brilliant blue dye to facilitate capsulotomy has been studied (Hisatomi et al, 2006;Udaondo et al, 2007;Terzariol et al, 2016)).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of equine corneal endothelium after exposure to 0.05% brilliant blue - an in vitro study

Andrade

Moreno

Muccillo

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the immediate effects of 0.05% brilliant blue on corneal endothelium of horses. Thirty-eight corneas of 19 horses, male or female, of different ages were studied. Corneas were randomly divided into two groups. Group 1: Corneal endothelium was covered with 0.3mL of brilliant blue 0.05% for 60 seconds followed by rinsing with a balanced salt solution. Group 2: Corneal endothelium was covered with BSS for 60 seconds. The corneas were excised with an 8mm trephine and prepared to analyze posterior endothelial surface using a light microscope (24 corneas) and a scanning electron microscope (14 corneas). The equine posterior corneal endothelium surface observed by optical microscopy and scanning electron microscopy revealed a continuous layer of polygonal cells of uniform size and shape in both the control and treatment groups. Due to non-normal residuals at ANOVA mean comparison, a generalized linear model was utilized at 5% level of significance. The chi-square test stated that treatment and control group were not different statistically. The 0.05% brilliant blue did not cause damage to equine corneal endothelium.

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Evaluation of the common pauraque (Nyctidromus albicollis) cornea using light and scanning electron microscopy

Tozetti,

de Lima Sousa Araújo,

Moreira

et al. 2023

Anat Histol Embryol

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The common pauraque Nyctidromus albicollis (Gmelin, 1789) is a widespread avian species; however due to its nocturnal habits and reclusive behaviour, little is known about their vision and ecology. Most avian species are visually dependent with advanced visual systems providing high spatial resolution, on the species needs. Each ocular structure has a specific role in contributing towards high visual function, and the cornea is the first refractive structure in the visual process. However, the common pauraque cornea had not been described until the present data. Therefore, this study aims to describe the morphology and morphometry of the common pauraque cornea by means of light and scanning electron microscopy to evaluate the cross‐sectional anatomy as well as the ultrastructure of the endothelial cells. Histological similarities with the cornea of other birds have been observed, but the thickness of the common pauraque cornea is much smaller than the other described corneas. A better understanding of the common pauraque cornea can help us better explain the physiology of vision and the visual requirements of this species. In turn, this will help us better understand how this species successfully interacts with its environment, and will improve our knowledge on how to interpret pathological changes in their cornea in a clinical setting.

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Análise morfométrica do endotélio corneano de coelhos à microscopia

Cited by 7 publications

References 12 publications

Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Evaluation of equine corneal endothelium after exposure to 0.05% brilliant blue - an in vitro study

Evaluation of the common pauraque (Nyctidromus albicollis) cornea using light and scanning electron microscopy

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