Anagen Hair Follicles Transplanted into Mature Human Scars Remodel Fibrotic Tissue

Plotczyk, Magdalena; Jiménez, Francisco; Boyle, Colin; Almquist, Benjamin D.

doi:10.2139/ssrn.3825157

Cited by 1 publication

(1 citation statement)

References 40 publications

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“…During the late anagen to middle telogen stage of hair follicle cycles, adipocytes tend to emit bone morphogenetic protein-2 (BMP-2), which helps the resting state of hair follicle stem cells and facilitates hair growth [12]. Recently, there has been increasing number of studies showing that dermal adipocytes are highly associated with hair follicles [13–17]. For example, Kim et al demonstrated that adipose-derive stem cells (ADSCs) secrete various growth factors, such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and fibroblast growth factor-1 and −2 (FGF-1and FGF-2), that have been shown to promote hair growth[18–22].…”

Section: Introductionmentioning

confidence: 99%

Intraoperative Bioprinting of Human Adipose-derived Stem cells and Extra-cellular Matrix Induces Hair Follicle-Like Downgrowths and Adipose Tissue Formation during Full-thickness Craniomaxillofacial Skin Reconstruction

Kang,

Yeo,

Derman

et al. 2023

Preprint

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Craniomaxillofacial (CMF) reconstruction is a challenging clinical dilemma. It often necessitates skin replacement in the form of autologous graft or flap surgery, which differ from one another based on hypodermal/dermal content. Unfortunately, both approaches are plagued by scarring, poor cosmesis, inadequate restoration of native anatomy and hair, alopecia, donor site morbidity, and potential for failure. Therefore, new reconstructive approaches are warranted, and tissue engineered skin represents an exciting alternative. In this study, we demonstrated the reconstruction of CMF full-thickness skin defects using intraoperative bioprinting (IOB), which enabled the repair of defects via direct bioprinting of multiple layers of skin on immunodeficient rats in a surgical setting. Using a newly formulated patient-sourced allogenic bioink consisting of both human adipose-derived extracellular matrix (adECM) and stem cells (ADSCs), skin loss was reconstructed by precise deposition of the hypodermal and dermal components under three different sets of animal studies. adECM, even at a very low concentration such as 2% or less, has shown to be bioprintable via droplet-based bioprinting and exhibitedde novoadipogenic capabilities bothin vitroandin vivo. Our findings demonstrate that the combinatorial delivery of adECM and ADSCs facilitated the reconstruction of three full-thickness skin defects, accomplishing near-complete wound closure within two weeks. More importantly, both hypodermal adipogenesis and downgrowth of hair follicle-like structures were achieved in this two-week time frame. Our approach illustrates the translational potential of using human-derived materials and IOB technologies for full-thickness skin loss.

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