Anaerobic treatment of tequila vinasses under seasonal operating conditions: Start-up, normal operation and restart-up after a long stop and starvation period

Jáuregui-Jáuregui, J.A.; Méndez‐Acosta, Hugo Oscar; González‐Álvarez, V.; Snell‐Castro, Raúl; Alcaraz-González, V.; Godon, Jean‐Jacques

doi:10.1016/j.biortech.2014.04.006

Cited by 36 publications

(26 citation statements)

References 30 publications

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“…Thus, Fig. 6 shows that values for Archaea and Bacteria were in the range of 3.2 Â 10 8 -9.5 Â 10 8 and 7.2 Â 10 8 -4.1 Â 10 9 (copies/mL) respectively, which agree with previously reported values for anaerobic digestion experiments treating complex substrates (Jáuregui-Jáuregui et al, 2014). Furthermore, Fig.…”

Section: Microbial Population Dynamicssupporting

confidence: 88%

“…Then, CE-SSCP analysis was performed in order to allow the separation of the amplicons according to their nucleotide base composition. The procedure and conditions of the analysis were previously described (Jáuregui-Jáuregui et al, 2014). The obtained CE-SSCP profiles were aligned with the internal standard ROX in order to correct any change in the electrophoretic motility between runs and the areas were normalized.…”

Section: Pcr Amplification and Ce-sscp Analysismentioning

confidence: 99%

“…The procedure and conditions of the PCR were applied as previously reported (Jáuregui-Jáuregui et al, 2014). Then, CE-SSCP analysis was performed in order to allow the separation of the amplicons according to their nucleotide base composition.…”

Section: Pcr Amplification and Ce-sscp Analysismentioning

confidence: 99%

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Methane production from acid hydrolysates of Agave tequilana bagasse: Evaluation of hydrolysis conditions and methane yield

Arreola-Vargas

Ojeda-Castillo

Snell‐Castro

et al. 2015

Bioresource Technology

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Section: Microbial Population Dynamicssupporting

confidence: 88%

Section: Pcr Amplification and Ce-sscp Analysismentioning

confidence: 99%

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Methane production from acid hydrolysates of Agave tequilana bagasse: Evaluation of hydrolysis conditions and methane yield

Arreola-Vargas

Ojeda-Castillo

Snell‐Castro

et al. 2015

Bioresource Technology

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“…The first one is a typical value found in AD process treating Tequila wastes (Jáuregui‐Jáuregui et al . ; Arreola‐Vargas et al . , ).…”

Section: Resultsmentioning

confidence: 99%

Active prokaryotic population dynamics exhibit high correlation to reactor performance during methane production from acid hydrolysates ofAgave tequilanavar.azulbagasse

Snell‐Castro¹,

Méndez‐Acosta²,

Arreola-Vargas³

et al. 2019

J Appl Microbiol

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Aims The purpose of this study was to apply cDNA approach for the characterization of active prokaryotic community to understand microbial scenarios and performance of an AnSBR digester fed with acid hydrolysates of Agave tequilana var. azul bagasse (ATAB). Methods and Results The digester was implemented for methane production under organic loading rate (OLR) disturbances to correlate physicochemical variables with changes in abundance, diversity and population dynamics of active Bacteria and Archaea by principal components analysis (PCA). Results indicated that methane yield increased as well as active syntrophic relationships for interspecies hydrogen/formate (Anaerolinaceae‐Methanobacterium beijingense) and acetate (Anaerolinaceae‐Methanosaeta concilii) transfers at 8 g‐COD l−1 day−1. However, methane yield was negatively affected at 16 g‐COD l−1 day−1 due to the competition for acetate by active Desulfovibrio marrakechensis and volatile fatty acids inhibition. Conclusions Microbial scenarios obtained by PCA correlations indicated that methane production from acid hydrolysates of ATAB was feasible at 8 g‐COD l−1 day−1. The digester operation at higher OLR only favoured methanogenesis by the hydrogenotrophic pathway. Significance and Impact of the Study Only cDNA analysis showed Archaea population dynamics, exhibiting high correlation with physicochemical variables towards the understanding of the methanogenic digester performance during OLR disturbances.

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“…Usually, CE-SSCP are processed and computed either with the graphical interface StatFingerprints program working under the R software environment (Michelland et al 2009) or the SAFUM toolbox written in MATLAB (Zemb et al 2007). The V3 variable region of the 16S rRNA gene is one of the most common target for characterizing archaeal and bacterial communities in anaerobic digesters (Zumstein et al 2000;Leclerc et al 2001Leclerc et al , 2004Mnif et al 2012;Jáuregui-Jáuregui et al 2014). In literature, SSCP analysis is widely reported as useful to compare microbial diversity in response to changes in environmental or process operation conditions especially when the diversity is low (Leclerc et al 2004;Ye et al 2007).…”

Section: Sscp Fingerprinting As a Rapid And Accurate Molecular Tool Fmentioning

confidence: 99%

How to use molecular biology tools for the study of the anaerobic digestion process?

Cabezas

Araújo

Callejas

et al. 2015

Rev Environ Sci Biotechnol

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Anaerobic digestion is used with success for the treatment of solid waste, urban and industrial effluents with a concomitant energy production. The process is robust and stable, but the complexity of the microbial community involved in the process is not yet fully comprehensive. Nowadays, the study of this complex ecosystem is facilitated by the availability of different molecular tools, but it is very important to choose the adequate tool to answer specific questions. The aim of this review is to describe different molecular techniques, indicate the questions that can be addressed by each technique, enumerate their limitations and give practical advices for their use. Examples of how the molecular tools have been used to address various questions in anaerobic digestion are presented. The key point now is to apply all this information to improve anaerobic digestion. The integration of concepts of microbial-ecology, environmental-engineering, modeling and bioinformatics is currently necessary.

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Anaerobic treatment of tequila vinasses under seasonal operating conditions: Start-up, normal operation and restart-up after a long stop and starvation period

Cited by 36 publications

References 30 publications

Methane production from acid hydrolysates of Agave tequilana bagasse: Evaluation of hydrolysis conditions and methane yield

Methane production from acid hydrolysates of Agave tequilana bagasse: Evaluation of hydrolysis conditions and methane yield

Active prokaryotic population dynamics exhibit high correlation to reactor performance during methane production from acid hydrolysates ofAgave tequilanavar.azulbagasse

How to use molecular biology tools for the study of the anaerobic digestion process?

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