Anaerobic propane oxidation in marine hydrocarbon seep sediments

Quistad, Steven D.; Valentine, David L.

doi:10.1016/j.gca.2011.02.001

Cited by 20 publications

(27 citation statements)

References 26 publications

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“…The results show that these microorganisms were apparently specialized, capable of degrading only propane and butane, similar to strain BuS5. This further supports in situ observations reporting that propane and butane appeared to be biologically degraded in oil and gas reservoirs (James and Burns, 1984;Boreham et al, 2001;Head et al, 2003;Larter et al, 2005) and at marine hydrocarbon-rich sites (Orcutt et al, 2010;Quistad and Valentine, 2011), whereas ethane, isobutane and pentane appeared rather resistant to degradation.…”

Section: Growth Tests With Other Hydrocarbonssupporting

confidence: 88%

“…These data include the 13 C-enrichment of propane and butane in sediment interstitial waters atop gas hydrates (Sassen et al, 2004) or at deep sea mud volcanoes (Mastalerz et al, 2009), sulfate-reduction rates much higher than could be accounted for by anaerobic oxidation of methane in hydrothermal sediments or at cold seeps Kallmeyer and Boetius, 2004;Orcutt et al 2004Orcutt et al , 2010Kleindienst et al, 2012), or the apparent consumption of shortchain alkanes at mud volcanoes (Niemann et al, 2006). In addition, oxidation of propane under anoxic conditions has been determined in sediments collected from marine gas seeps (Quistad and Valentine, 2011). Anaerobic degradation of shortchain alkanes was demonstrated recently with a pure culture of a marine sulfate-reducing bacterium strain BuS5, isolated from Guaymas Basin hydrothermal vent sediments (Kniemeyer et al, 2007), and with several enrichment cultures obtained from sediments around marine and terrestrial hydrocarbon seeps (Kniemeyer et al, 2007;Savage et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps

et al. 2012

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The short-chain, non-methane hydrocarbons propane and butane can contribute significantly to the carbon and sulfur cycles in marine environments affected by oil or natural gas seepage. In the present study, we enriched and identified novel propane and butane-degrading sulfate reducers from marine oil and gas cold seeps in the Gulf of Mexico and Hydrate Ridge. The enrichment cultures obtained were able to degrade simultaneously propane and butane, but not other gaseous alkanes. They were cold-adapted, showing highest sulfate-reduction rates between 16 and 20 1C. Analysis of 16S rRNA gene libraries, followed by whole-cell hybridizations with sequence-specific oligonucleotide probes showed that each enrichment culture was dominated by a unique phylotype affiliated with the Desulfosarcina-Desulfococcus cluster within the Deltaproteobacteria. These phylotypes formed a distinct phylogenetic cluster of propane and butane degraders, including sequences from environments associated with hydrocarbon seeps. Incubations with 13 C-labeled substrates, hybridizations with sequence-specific probes and nanoSIMS analyses showed that cells of the dominant phylotypes were the first to become enriched in 13 C, demonstrating that they were directly involved in hydrocarbon degradation. Furthermore, using the nanoSIMS data, carbon assimilation rates were calculated for the dominant cells in each enrichment culture.

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Section: Growth Tests With Other Hydrocarbonssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps

et al. 2012

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“…Note that propane is highly susceptible to microbial attack, which will result in an anomalously heavy isotopic value [32,75,76].…”

Section: Compound-specific Isotopesmentioning

confidence: 99%

Evaluation of Near-Surface Gases in Marine Sediments to Assess Subsurface Petroleum Gas Generation and Entrapment

Abrams

2017

Geosciences

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Gases contained within near-surface marine sediments can be derived from multiple sources: shallow microbial activity, thermal cracking of organic matter and inorganic materials, or magmatic-mantle degassing. Each origin will display a distinctive hydrocarbon and non-hydrocarbon composition as well as compound-specific isotope signature and thus the interpretation of origin should be relatively straightforward. Unfortunately, this is not always the case due to in situ microbial alteration, non-equilibrium phase partitioning, mixing, and fractionation related to the gas extraction method. Sediment gases can reside in the interstitial spaces, bound to mineral or organic surfaces and/or entrapped in carbonate inclusions. The interstitial sediment gases are contained within the sediment pore space, either dissolved in the pore waters (solute) or as free (vapour) gas. The bound gases are believed to be attached to organic and/or mineral surfaces, entrapped in structured water or entrapped in authigenic carbonate inclusions. The purpose of this paper is to provide a review of the gas types found within shallow marine sediments and examine issues related to gas sampling and extraction. In addition, the paper will discuss how to recognise mixing, alteration and fractionation issues to best interpret the seabed geochemical results and determine gas origin to assess subsurface petroleum gas generation and entrapment.

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“…For example, in this work we used a mixture of acetate, propionate and butyrate because they are used by many sulfate reducers and in the sea are easily found short chain fatty acids that serve as electron donors for sulfate reduction and also they are used by fermenting bacteria 5,6 . We would recommend combinations such as: acetate-butyrate, propionatebutyrate or only butyrate.…”

Section: Discussionmentioning

confidence: 99%

“…The cultivation of the sediments to obtain the sludge was conducted by using a mixture of acetate, propionate and butyrate as substrate because these volatile fatty acids are used by several strains of sulfate reducing bacteria. These acids are also the type of carbon compounds frequently found in marine sediments, according to several reports in literature on carbonaceous material in sea sediments 5,6 . Finally, some of the most toxic compounds that are found in groundwater and other water bodies around the world are the chlorinated solvents such as trichloroethylene (TCE) or perchloroethylene (PCE).…”

Section: Introductionmentioning

confidence: 86%

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Guerrero–Barajas

Ordaz

García-Solares

et al. 2015

JoVE

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The importance of microbial sulfate reduction relies on the various applications that it offers in environmental biotechnology. Engineered sulfate reduction is used in industrial wastewater treatment to remove large concentrations of sulfate along with the chemical oxygen demand (COD) and heavy metals. The most common approach to the process is with anaerobic bioreactors in which sulfidogenic sludge is obtained through adaptation of predominantly methanogenic granular sludge to sulfidogenesis. This process may take a long time and does not always eliminate the competition for substrate due to the presence of methanogens in the sludge. In this work, we propose a novel approach to obtain sulfidogenic sludge in which hydrothermal vents sediments are the original source of microorganisms. The microbial community developed in the presence of sulfate and volatile fatty acids is wide enough to sustain sulfate reduction over a long period of time without exhibiting inhibition due to sulfide. This protocol describes the procedure to generate the sludge from the sediments in an upflow anaerobic sludge blanket (UASB) type of reactor. Furthermore, the protocol presents the procedure to demonstrate the capability of the sludge to remove by reductive dechlorination a model of a highly toxic organic pollutant such as trichloroethylene (TCE). The protocol is divided in three stages: (1) the formation of the sludge and the determination of its sulfate reducing activity in the UASB, (2) the experiment to remove the TCE by the sludge, and (3) the identification of microorganisms in the sludge after the TCE reduction. Although in this case the sediments were taken from a site located in Mexico, the generation of a sulfidogenic sludge by using this procedure may work if a different source of sediments is taken since marine sediments are a natural pool of microorganisms that may be enriched in sulfate reducing bacteria. Video LinkThe video component of this article can be found at

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Anaerobic propane oxidation in marine hydrocarbon seep sediments

Cited by 20 publications

References 26 publications

Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps

Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps

Evaluation of Near-Surface Gases in Marine Sediments to Assess Subsurface Petroleum Gas Generation and Entrapment

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

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