An UPLC-MS/MS Method for the Simultaneous Identification and Quantitation of Cell Wall Phenolics in Brassica napus Seeds

Frolov, Andrej; Henning, Anja; Böttcher, Christoph; Tissier, Alain; Strack, Dieter

doi:10.1021/jf3042648

Cited by 29 publications

(23 citation statements)

References 42 publications

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“…Phenolic compounds play diverse roles in plants. These compounds provide structural support of the cell wall or protect plants against pathogens, herbivores, and UV radiation [17]. Of all classes of secondary metabolites, phenolics, specifically flavonoids, are the most relevant for UV protection.…”

Section: Introductionmentioning

confidence: 99%

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

Liu

Abozeid

et al. 2020

Biomolecules

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Ultraviolet-B (UV-B) radiation (280–320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two Astragalus varieties prescribed by the Chinese Pharmacopoeia, Astragalus mongholicus has better tolerance to UV-B. Thus, it is necessary to study the metabolic strategy of Astragalus under UV-B radiation further. In the present study, we used untargeted gas chromatography-mass spectrometry (GC-MS) and targeted liquid chromatography-mass spectrometry (LC-MS techniques) to investigate the profiles of primary and secondary metabolic. The profiles revealed the metabolic response of Astragalus to UV-B radiation. We then used real-time polymerase chain reaction (RT-PCR) to obtain the transcription level of relevant genes under UV-B radiation (UV-B supplemented in the field, λmax = 313 nm, 30 W, lamp-leaf distance = 60 cm, 40 min·day−1), which annotated the responsive mechanism of phenolic metabolism in roots. Our results indicated that supplemental UV-B radiation induced a stronger shift from carbon assimilation to carbon accumulation. The flux through the phenylpropanoids pathway increased due to the mobilization of carbon reserves. The response of metabolism was observed to be significantly tissue-specific upon the UV-B radiation treatment. Among phenolic compounds, C6C1 carbon compounds (phenolic acids in leaves) and C6C3C6 carbon compounds (flavones in leaves and isoflavones in roots) increased at the expense of C6C3 carbon compounds. Verification experiments show that the response of phenolics in roots to UV-B is activated by upregulation of relevant genes rather than phenylalanine. Overall, this study reveals the tissues-specific alteration and mechanism of primary and secondary metabolic strategy in response to UV-B radiation.

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Section: Introductionmentioning

confidence: 99%

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

Liu

Abozeid

et al. 2020

Biomolecules

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“…Due to their cis-diol groups, these metabolites saturate the binding sites of the affinity column and reduce the peptide enrichment efficiency. Moreover, phenylpropanoids, as well as their sugar esters and glycosides, are well-retained on reversed phase [63] and might co-elute with peptides in course of RP-HPLC separation, leading to essential ion suppression during the ESI process and shift of peptide signals in the lower part on the instrument dynamic range. Thus, the analysis of early glycation proteins in total extracts that can not be efficiently ultrafiltrated is a challenging task.…”

Section: Lc-ms Analysis Of Glycated Peptide Mixturesmentioning

confidence: 99%

Glycation of Plant Proteins under Environmental Stress — Methodological Approaches, Potential Mechanisms and Biological Role

Bilova¹,

Greifenhagen²,

Paudel³

et al. 2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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Environmental stress is one of the major factors reducing crop productivity. Due to the oncoming climate changes, the effects of drought and high light on plants play an increasing role in modern agriculture. These changes are accompanied with a progressing contamination of soils with heavy metals. Independent of their nature, environmental alterations result in development of oxidative stress, i.e. increase of reactive oxygen species ROS contents, and metabolic adjustment, i.e. accumulation of soluble primary metabolites amino acids and sugars . However, a simultaneous increase of ROS and sugar concentrations ultimately results in protein glycation, i.e. non-enzymatic interaction of reducing sugars or their degradation products α-dicarbonyls with proteins. The eventually resulting advanced glycation end-products "GEs are known to be toxic and pro-inflammatory in mammals. Recently, their presence was unambiguously demonstrated in vivo in stressed Arabidopsis thaliana plants. Currently, information on protein targets, modification sites therein, mediators and mechanisms of plant glycation are being intensively studied. In this chapter, we comprehensively review the methodological approaches for plant glycation research and discuss potential mechanisms of "GE formation under stress conditions. On the basis of these patterns and additional in vitro experiments, the pathways and mechanisms of plant glycation can be proposed.

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“…Rapeseed ( Brassica napus; Cruciferae ) is an important oil crop in agriculture worldwide, and ranks the third largest source of vegetable oil all over the world [1,2,3]. Traditional rapeseed oil contains more than 40% erucic acid [4], which could pose a risk to human health when excessive amounts are consumed [5].…”

Section: Introductionmentioning

confidence: 99%

“…Metabolomics was used to investigate the chemical composition of different organs of Brassica napus L. using UPLC-QTOF-PDAP-MS [17]. The composition of oilseed rape and turnip rape seeds has been investigated by nuclear magnetic resonance (NMR) [18] and the cell wall phenolics by UPLC-MS/MS [1]. Comparative metabolomics was employed to evaluate Chinese and North American rice [16], analyze carrots from different agronomic environments [19], distinguish Zingiber officinale Roscoe from two geographical origins [20], expound the profile of fruits from Tanzania [15], and characterize the metabolic changes of cultivated and wild soybean under salt stress [21].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Analysis of Rapeseeds from Three Countries

et al. 2019

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Rapeseed is an important oilseed with proper fatty acid composition and abundant bioactive components. Canada and China are the two major rapeseed-producing countries all over the world. Meanwhile, Canada and Mongolia are major importers of rapeseed due to the great demand for rapeseed in China. To investigate the metabolites in rapeseeds from three countries, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics was employed to analyze rapeseeds from China, Canada, and Mongolia. As results, 67, 53, and 68 metabolites showed significant differences between Chinese and Canadian, Chinese and Mongolian, and Canadian and Mongolian rapeseeds, respectively. Differential metabolites were mainly distributed in the metabolic pathways including phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis. Among the differential metabolites, contents of sinapate and sinapine were higher in Chinese rapeseeds, while the contents of brassicasterol, stigmasterol, and campestanol were higher in Canadian rapeseeds. These findings might provide insight into the metabolic characteristics of rapeseeds from three countries to guide processing and consumption of the products of rapeseed.

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An UPLC-MS/MS Method for the Simultaneous Identification and Quantitation of Cell Wall Phenolics in Brassica napus Seeds

Cited by 29 publications

References 42 publications

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

Glycation of Plant Proteins under Environmental Stress — Methodological Approaches, Potential Mechanisms and Biological Role

Comparative Metabolomic Analysis of Rapeseeds from Three Countries

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