An update on microbial carotenoid production: application of recent metabolic engineering tools

Das, A.; Yoon, Sang-Hwal; Lee, Sook-Hee; Kim, Jae‐Yean; Oh, Deok‐Kun; Kim, Seon-Won

doi:10.1007/s00253-007-1206-3

Cited by 184 publications

(100 citation statements)

References 52 publications

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“…For both pathways, the common precursor, C 40 lycopene, is synthesized from C 15 farnesyl pyrophosphate (FPP) via the methylerythritol 4-phosphate (MEP) pathway, which is present in most eubacteria (33). Effective lycopene production has been achieved in genetically engineered noncarotenogenic hosts, such as Escherichia coli and Saccharomyces cerevisiae (9). Accordingly, the potential of using such biotechnologically relevant hosts for heterologous production of any lycopene-derived carotenoids has generated high interest.…”

mentioning

confidence: 99%

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases

et al. 2010

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We report the cloning and characterization of the biosynthetic gene cluster (crtE, crtB, crtI, crtE2, crtYg, crtYh, and crtX) of the ␥-cyclic C 50 carotenoid sarcinaxanthin in Micrococcus luteus NCTC2665. Expression of the complete and partial gene cluster in Escherichia coli hosts revealed that sarcinaxanthin biosynthesis from the precursor molecule farnesyl pyrophosphate (FPP) proceeds via C 40 lycopene, C 45 nonaflavuxanthin, C 50 flavuxanthin, and C 50 sarcinaxanthin. Glucosylation of sarcinaxanthin was accomplished by the crtX gene product. This is the first report describing the biosynthetic pathway of a ␥-cyclic C 50 carotenoid. Expression of the corresponding genes from the marine M. luteus isolate Otnes7 in a lycopene-producing E. coli host resulted in the production of up to 2.5 mg/g cell dry weight sarcinaxanthin in shake flasks. In an attempt to experimentally understand the specific difference between the biosynthetic pathways of sarcinaxanthin and the structurally related -cyclic decaprenoxanthin, we constructed a hybrid gene cluster with the ␥-cyclic C 50 carotenoid cyclase genes crtYg and crtYh from M. luteus replaced with the analogous -cyclic C 50 carotenoid cyclase genes crtYe and crtYf from the natural decaprenoxanthin producer Corynebacterium glutamicum. Surprisingly, expression of this hybrid gene cluster in an E. coli host resulted in accumulation of not only decaprenoxanthin, but also sarcinaxanthin and the asymmetric -and ␥-cyclic C 50 carotenoid sarprenoxanthin, described for the first time in this work. Together, these data contributed to new insight into the diverse and multiple functions of bacterial C 50 carotenoid cyclases as key catalysts for the synthesis of structurally different carotenoids.

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mentioning

confidence: 99%

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases

et al. 2010

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“…They contain no expensive or rare metals and can be obtained by extraction from plants or by expression in microbes [7,8]. Furthermore, they often possess higher extinction coefficients than conventional metal complex dyes.…”

Section: Preparation Of Dscsmentioning

confidence: 99%

Impact of photocatalysis on carotenoic acid dye-sensitized solar cells

Jöhr¹,

Zając²,

Günzburger³

et al. 2015

Hybrid Materials

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Sensitized mesoporous titania is of increasing interest for catalysis and photovoltaic devices such as dye-sensitized solar cells (DSCs). For photovoltaic applications, the catalytic properties of TiO2 can cause degradation of the dyes during device fabrication. This is especially the case if natural sensitizers are used. We addressed this issue by fabrication of carotenoic acid sensitized solar cells under inert and ambient assembly conditions. The DSCs were investigated by currentvoltage and quantum efficiency measurements. Further characterization of the cells was made using impedance spectroscopy. The conversion efficiency of the DSCs prepared under inert conditions improved by at least 25% and the devices showed an enhanced reproducibility. The improvement of the DSCs correlated with the conversion efficiency of the sensitizers under inert conditions. We conclude that the photocatalytic bleaching depends on the electron injection efficiency of the sensitizer. Hence carotenoic acids support their own degradation. However, the photocatalytic decomposition of the sensitizers can be avoided by fabrication of the DSCs under inert conditions.

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“…The reactions catalyzed by DXS and IDI have been reported as the most critical rate-limiting step in the endogenous MEP pathway of E. coli [27][28][29][30]. In this study, the polycistronic operon dxs-idi was constructed based on pTrcHis2B, pQE30 and pET21a + to form p40Trc-dxs-idi, p40T5-dxs-idi and p40T7-dxs-idi.…”

Section: Overexpression Of Rate-limiting Enzymes In the Endogenous Mementioning

confidence: 99%

Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli

et al. 2014

Bioresour. Bioprocess.

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Background: Limonene is an important monoterpene used as a chemical commodity and precursor for producing biofuels, flavor and medicinal compounds. Results: In this paper, we engineered Escherichia coli by embedding two exogenous genes encoding a limonene synthase (LS) and a geranyl diphosphate synthase (GPPS) for production of limonene. Out of 12 E. coli strains transformed with various plasmids, the best one with p15T7-ls-gpps produced limonene with a titer of 4.87 mg/L. In order to enhance the limonene production, two rate-limiting enzymes in the endogenous MEP pathway of E. coli, 1-deoxy-xylulose-5-phosphate synthase (DXS) and isopentenyl diphosphate isomerase (IDI), were overexpressed consecutively on vector pET21a+, resulting in a production of 17.4 mg limonene /L at 48 h. Conclusions: After the preliminary optimization of the medium in a two-phase culture system composed of n-hexadecane (1/50, V org /V aq ), the final production of limonene was raised up to 35.8 mg/L, representing approximately a 7-fold improvement compared to the initial titer.

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An update on microbial carotenoid production: application of recent metabolic engineering tools

Cited by 184 publications

References 52 publications

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases

Impact of photocatalysis on carotenoic acid dye-sensitized solar cells

Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli

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An update on microbial carotenoid production: application of recent metabolic engineering tools

Cited by 184 publications

References 52 publications

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C 50 Carotenoid Cyclases

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C 50 Carotenoid Cyclases

Impact of photocatalysis on carotenoic acid dye-sensitized solar cells

Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli

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Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases

Biosynthetic Pathway for γ-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C ₅₀ Carotenoid Cyclases