An ultrastructural study of Porphyromonas gingivalis-induced platelet aggregation

Buerger Disease, Smoking, and Periodontitis

“…On the other hand, the bacteria captured by granulocytes were clearly killed within one hour. These observations confirmed that P. gingivalis bacteria aggregate strongly in the platelets, enter the platelets, and live without dying 12) ( Figs. 1, 2 ).…”

“…This suggested that the healthy small arteries of the rats could become embolized by platelet aggregation, as shown in animal experiments. 12) Moreover, clinically in Buerger disease, the healthy but spastic arterial lumen of the fingers or toes could be occluded through sudden onset.…”

An Experimental Model of Peripheral Vascular Disease Involving the Intravenous Injection of Oral Bacteria

“…As a result, we were able to show that Pg caused platelet activation and cohesion, which might have led to clot formation. 15) This was also proven via experiments involving rats. Specifically, we embedded subcutaneous pumps into the jugular veins of rats, which continuously infused Pg.…”

Are There Any Objections against Our Hypothesis That Buerger Disease Is an Infectious Disease?

“…Oral bacteria DNA from periodontal source were observed in arterial matter of 93% 4) of Buerger disease patients. In addition, we found the possible transportation system of the oral bacteria dwelling in the platelet, 5) and we also knew that the oral bacteria aggregates platelets very strongly. 5) A genetic study, 6) epidemiologic factors, 7) and an antibody titer study 8) supported our hypothesis.…”

“…The aggregated mass reaches over 100 micrometers in vitro. 5) The embolic phenomenon is the principle of Buerger disease that was emphasized by Dr Buerger. 11) Distal areas such as the big toe or little toe are common targets of necrosis.…”