An ultrasensitive micropillar-based quartz crystal microbalance device for real-time measurement of protein immobilization and protein-protein interaction

Su, Jun-Wei; Esmaeilzadeh, Hamed; Zhang, Fang; Yu, Qing; Cernigliaro, George; Xu, Jin; Sun, Hongwei

doi:10.1016/j.bios.2017.07.074

Cited by 32 publications

(11 citation statements)

References 46 publications

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“…Each silicon atom was coordinated with four oxygen atoms. 4 , and H 1s 1 . Before adsorption, the H2O was placed inside a 10 Å × 10 Å × 10 Å cubic cell for an optimization calculation.…”

Section: Quartz Crystal Cell Optimizationmentioning

confidence: 98%

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Structural and Electronic Properties of Different Terminations for Quartz (001) Surfaces as Well as Water Molecule Adsorption on It: A First-Principles Study

Wang

Zhang

et al. 2018

Minerals

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Structural and electronic properties of Si termination, O-middle termination, and O-rich terminations of a quartz (001) surface as well as water molecule adsorption on it were simulated by means of density functional theory (DFT). Calculated results show that the O-middle termination exposing a single oxygen atom on the surface is the most stable model of quartz (001) surface, with the lowest surface energy at 1.969 J·m −2 , followed by the O-rich termination and Si termination at 2.892 J·m −2 and 2.896 J·m −2 , respectively. The surface properties of different terminations mainly depend on the surface-exposed silicon and oxygen atoms, as almost all the contributions to the Fermi level (E F ) in density of states (DOS) are offered by the surface-exposed atoms, especially the O2p state. In the molecular adsorption model, H 2 O prefers to adsorb on the surface Si and O atoms, mainly via O 1 -H 1 bond at 1.259 Å and Si 1 -O w at 1.970 Å by Van der Waals force and weak hydrogen bond with an adsorption energy of −57.89 kJ·mol −1 . In the dissociative adsorption model, the O-middle termination is hydroxylated after adsorption, generating two new Si-OH silanol groups on the surface and forming the O w H 2 ···O 4 hydrogen bond at a length of 2.690 Å, along with a large adsorption energy of −99.37 kJ·mol −1 . These variations in the presence of H 2 O may have a great influence on the subsequent interfacial reactions on the quartz surface.

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Section: Quartz Crystal Cell Optimizationmentioning

confidence: 98%

“…Quartz is an important mineral that has wide applications as a raw material in various fields in industry, such as glass, ceramics, and medicine [1][2][3]. It is the main component mineral of a series of important mineral resources such as siliceous phosphate ore, iron ore, tungsten ore, fluorite, and mica.…”

Section: Introductionmentioning

confidence: 99%

Structural and Electronic Properties of Different Terminations for Quartz (001) Surfaces as Well as Water Molecule Adsorption on It: A First-Principles Study

Wang

Zhang

et al. 2018

Minerals

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“…In this framework, lipid‐based synthetic model membranes are useful platforms to mimic biological interfaces under simplified conditions, allowing for the identification of key determinants regulating nano‐bio interactions (Gkeka et al ., 2013; Simonelli et al ., 2015; Su et al ., 2018). Supported lipid bilayers (SLBs) are often used as 2D biomembrane models (Richter et al ., 2006; Hardy et al ., 2013), enabling to precisely tune their physicochemical properties and avoiding the complications related to the 3D nature of biological membranes.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

Ridolfi

Caselli

Montis

et al. 2020

Journal of Microscopy

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Inorganic nanoparticles (NPs) represent promising examples of engineered nanomaterials, providing interesting biomedical solutions in several fields, like therapeutics and diagnostics. Despite the extensive number of investigations motivated by their remarkable potential for nanomedicinal applications, the interactions of NPs with biological interfaces are still poorly understood. The effect of NPs on living organisms is mediated by biological barriers, such as the cell plasma membrane, whose lateral heterogeneity is thought to play a prominent role in NPs adsorption and uptake pathways. In particular, biological membranes feature the presence of rafts, that is segregated lipid micro and/or nanodomains in the so-called liquid ordered phase (L o), immiscible with the surrounding liquid disordered phase (L d). Rafts are involved in various biological functions and act as sites for the selective adsorption of materials on the membrane. Indeed, the thickness mismatch present along their boundaries generates energetically favourable conditions for the adsorption of NPs. Despite its clear implications in NPs internalisation processes and cytotoxicity, a direct proof of the selective adsorption of NPs along the rafts' boundaries is still missing to date. Here we use multicomponent supported lipid bilayers (SLBs) as reliable synthetic models, reproducing the nanometric lateral heterogeneity of cell membranes. After being characterised by atomic force microscopy (AFM) and neutron reflectivity (NR), multidomain SLBs are challenged by prototypical inorganic nanoparticles, that is citrated gold nanoparticles (AuNPs), under simplified and highly controlled conditions. By exploiting AFM, we demonstrate that AuNPs preferentially target lipid phase

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“…QCM is a commercially available device for measuring biomolecules with weight within the nanogram to microgram range. Although QCM facilitates the analysis of disease biomarkers, various analytes, pathogens, environmental pollutants, and food contaminants, 20,41 it suffers from low sensitivity 41 . To enhance its sensitivity (which requires thinner and fragile quartz crystal), the coupling of polymer micropillars with QCM has been discovered as a game‐changer 42,43 .…”

Section: Introductionmentioning

confidence: 99%

“…Although QCM facilitates the analysis of disease biomarkers, various analytes, pathogens, environmental pollutants, and food contaminants, 20,41 it suffers from low sensitivity. 41 To enhance its sensitivity (which requires thinner and fragile quartz crystal), the coupling of polymer micropillars with QCM has been discovered as a game-changer. 42,43 The micropillar-based QCM has now been reported for the detection of hydrogen, 44 and humidity detection.…”

mentioning

confidence: 99%

Modeling and analysis of nature‐inspired branched micropillars for enhanced dynamic bio‐sensing

Mustapha

Hawwa

Abakr

2021

Numer Methods Biomed Eng

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Research evidence abounds on the effectiveness of micropillar‐based microelectromechanical systems for the detection of a wide variety of ultrasmall biological objects for clinical and non‐clinical applications. However, the standard micropillar‐based sensing platforms rely on a single‐column micropillar with a spot at the tip for binding of objects. Although this long‐standing form has shown immense potential, performance improvement is hindered by the fundamental limits enforced by physical laws. Moreover, the single‐column micropillar has a lower sensing area and is ill‐suited for a simultaneous differential sensing of chemical/biological objects of different mass. Here, we report a new set of nature‐inspired, branched micropillar‐based sensing resonators to address the highlighted issues. The characteristics of the newly proposed branched micropillars are comprehensively examined with three payloads (Bartonella Bacilliformis, Escherichia coli, and Micro magnetic beads). Anchored on the capability of continuum theoretical framework, the mathematical model of the micropillar is formulated through the synthesis of the modified couple stress, the Rayleigh‐Love, and the Timoshenko theories. The finite element method is employed to shed light on the variability of the structures' resonant response under performance reduction factors (payload's rotary inertia, damaged substrate, and density of a surrounding fluid). The results obtained indicate superior performance indicators for the triply‐branched micropillar: enhanced response sensitivity for multiple payloads and less susceptibility to deterioration in resonant frequencies due to fluid immersion.

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An ultrasensitive micropillar-based quartz crystal microbalance device for real-time measurement of protein immobilization and protein-protein interaction

Cited by 32 publications

References 46 publications

Structural and Electronic Properties of Different Terminations for Quartz (001) Surfaces as Well as Water Molecule Adsorption on It: A First-Principles Study

Structural and Electronic Properties of Different Terminations for Quartz (001) Surfaces as Well as Water Molecule Adsorption on It: A First-Principles Study

Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

Modeling and analysis of nature‐inspired branched micropillars for enhanced dynamic bio‐sensing

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