An overview of most commonly used methods for detection of fish mislabeling

Kudra, Enida Članjak –; Fazlović, Neira; Alagić, Davor; Smajlović, Muhamed; Čaklovica, Kenan; Smajlović, Ahmed

doi:10.51607/22331360.2021.70.2.169

Cited by 2 publications

(3 citation statements)

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“…Following this, electrophoresis is conducted using a permeable acrylamide gel matrix, which effectively separates proteins based on their molecular mass with high precision (Walker, 1996). The SDS detergent is appropriate for extracting denatured proteins in studied materials, making it a good approach for determining species that have undergone heat treatment (Članjak-Kudra et al, 2021).…”

Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis (S...mentioning

confidence: 99%

“…The variation in electrophoretic mobilities of the compounds under investigation within a highpotential-exposed narrow-silica capillary column (50-150 μm in diameter) determines the outcome. While the mass-to-charge ratio does not change, the molecules are categorized based on their different masses (Članjak-Kudra et al, 2021). According to Lago et al (2014), this automated method can be utilized for commercial purposes due to its relatively fast analysis time of only about 10 minutes.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

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Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Tokur,

Korkmaz

2023

Foodb

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The identification of the species of seafood and their products, whether they are fresh or cooked, is one of the key concerns of food regulations in many countries that have a significant intake of seafood. In point of fact, a commercial fraud happens when a species that is less value is intentionally substituted for a species that is more valuable, and a sanitary fraud takes place when a product that is potentially harmful is introduced into the market. A primary responsibility of veterinary inspection of seafood products is the detection of harmful species with the aim of removing them from the retail trade (Council Directive 91/493/ECC). Two efficient methods for seafood species identification are molecular biology methods and protein electrophoresis. Classic electrophoretic methods have been used for a long time to authenticate seafood; they are simple, accurate, and inexpensive compared to molecular biological methods, which are the wave of the future in food safety labs. The purpose of this article is to offer an overview of the electrophoretic methods commonly used to identify different species of seafood, including sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), native or urea-isoelectric focusing electrophoresis (IEF), two-dimensional electrophoresis (2-DE), and capillary electrophoresis (CE).

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Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis (S...mentioning

confidence: 99%

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Tokur,

Korkmaz

2023

Foodb

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“…Although protein-based analysis has some edges, particularly in the practical sense, such as the availability of various protein kit analyses and relatively affordable, it has a major drawback when compared to DNA-based analysis, especially for products that have been extensively processed [10]. This is because DNA is much more stable under extreme conditions and not easily denatured [11]. Therefore, DNA-based analysis using polymerase chain reaction (PCR) remains the most popular method to distinguish contamination of non-halal meat in food products.…”

Section: ■ Introductionmentioning

confidence: 99%

Primer Pairs Specificity Test for Frog Meat Identification Using PCR Technique

Haryono,

Khusufi,

Pangesti

et al. 2023

Indones. J. Chem.

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Halal food assurance is becoming more important with the growth of the halal industry globally. Adulteration of halal meat products using non-halal sources such as pork, dog, boar, and even frog meat has become a major problem for moslems. The purpose of this study is to initiate the method for frog meat identification using polymerase chain reaction (PCR) technique. In this study, three primer pairs (Fk1-Rk1, Fk2-Rk2, Fk3-Rk3) were analyzed for their specificity toward frog meat against other common halal meat sources such as beef, chicken, shrimp, squid, and mackerel. The visualization of DNA amplification showed that primer pair Fk1-Rk1 produced primer-dimer, thus cannot be used for this circumstance. Primer pair Fk2-Rk2 showed a better result where DNA amplicon was produced at ~100 bp for frog meat and no amplicons for other meat. Primer pair Fk3-Rk3 showed a different pattern of DNA amplification for all the meat tested, where the amplicon of frog meat was shown at ~100 bp, while the other meat showed multiple amplicons or none. In conclusion, primer pairs Fk2-Rk2 and Fk3-Rk3 showed their potential as primer pairs for frog meat identification using PCR for implementing halal food assurance, although sensitivity analysis needs to be investigated.

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An overview of most commonly used methods for detection of fish mislabeling

Cited by 2 publications

References 0 publications

Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Primer Pairs Specificity Test for Frog Meat Identification Using PCR Technique

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