An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability

Boularaoui, Selwa Mokhtar; Khan, Kamran A.; Christoforou, Nicolas; Stefanini, Cesare

doi:10.1016/j.bprint.2020.e00093

Cited by 166 publications

(127 citation statements)

References 157 publications

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“…), desired feature size, resolution, throughput, and speed[ 13 ]. In biomedical 3DP extrusion[ 15 ], thermal inkjet and laser-assisted techniques are commonly used. The most common techniques for metal 3DP are powder bed fusion or melting methods, among which the selective laser melting (SLM) and electron beam melting (EBM) are very popular and they are the most widely used 3DP methods in the world.…”

Section: 3dp Process Techniquesmentioning

confidence: 99%

3D Printing Technologies in Metallic Implants: A Thematic Review on the Techniques and Procedures

Attarilar¹,

Ebrahimi²,

Djavanroodi³

et al. 2024

IJB

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Additive manufacturing (AM) is among the most attractive methods to produce implants, the processes are very swift and it can be precisely controlled to meet patient’s requirement since they can be produced in exact shape, dimension, and even texture of different living tissues. Until now, lots of methods have emerged and used in this field with diverse characteristics. This review aims to comprehensively discuss 3D printing (3DP) technologies to manufacture metallic implants, especially on techniques and procedures. Various technologies based on their main properties are categorized, the effecting parameters are introduced, andthe history of AM technology is briefly analyzed. Subsequently, the utilization of these AM-manufactured components in medicine along with their effectual variables is discussed, and special attention is paid on to the production of porous scaffolds, taking pore size, density, etc., into consideration. Finally, 3DP of the popular metallic systems in medical applications such as titanium, Ti6Al4V, cobalt-chromium alloys, and shape memory alloys are studied. In general, AM manufactured implants need to comply with important requirements such as biocompatibility, suitable mechanical properties (strength and elastic modulus), surface conditions, custom-built designs, fast production, etc. This review aims to introduce the AM technologies in implant applications and find new ways to design more sophisticated methods and compatible implants that mimic the desired tissue functions.

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Section: 3dp Process Techniquesmentioning

confidence: 99%

3D Printing Technologies in Metallic Implants: A Thematic Review on the Techniques and Procedures

Attarilar¹,

Ebrahimi²,

Djavanroodi³

et al. 2024

IJB

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“…Instead, constructs can be composed of independently extruded hydrogels each containing specific cell types of interest. However, extrusion of cell-containing hydrogels exerts shear stress on the cells to reduce cell viability 12 . Here, we determined to assess the viability of MDA-MB-231 cells after they had been extruded in laminin bioink either through an 18 G nozzle or an 18 G needle as compared to the viability of cells that had simply been dispersed in the bioink.…”

Section: Viability Of Cells Extruded In Laminin Bioinkmentioning

confidence: 99%

An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multi-material bioprinting

Engberg

Stelzl

Eriksson

et al. 2021

Preprint

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Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatment of diseases such as cancer. However, the rapidly expanding bioprinting research field is currently limited by access to bioprinters. To increase the availability of bioprinting technologies we present here an open source extrusion bioprinter based on the E3D motion system and tool changer to enable high-resolution multi-material bioprinting. As proof of concept, the bioprinter is used to create collagen constructs using freeform reversible embedding of suspended hydrogels (FRESH) methodology, as well as multi-material constructs composed of distinct sections of laminin and collagen. Data is presented demonstrating that the bioprinted constructs support growth of cells either seeded onto printed constructs or included in the bioink prior to bioprinting. This open source bioprinter is easily adapted for different bioprinting applications, and additional tools can be incorporated to increase the capabilities of the system.

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“…Hydrogel composition together with printing parameters strongly affect printability in terms of desired shape resolution, as demonstrated using pure alginate or alginate with gelatin and methyl-cellulose [195]. Moreover, the solvent used for hydrogel formation, the resultant viscosity, the applied pressure and temperature, as well as the nozzle size and geometrical factors of the printing head all influence cell viability, due to the shear stress experienced by the cells upon extrusion, which represents a key factor [196]. Shear-thinning properties are crucial for keeping bioink shape fidelity and cell viability.…”

Section: D (Bio-)printing: Cutting Edge Applications and Future Persmentioning

confidence: 99%

Four-Dimensional (Bio-)printing: A Review on Stimuli-Responsive Mechanisms and Their Biomedical Suitability

et al. 2020

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The applications of tissue engineered constructs have witnessed great advances in the last few years, as advanced fabrication techniques have enabled promising approaches to develop structures and devices for biomedical uses. (Bio-)printing, including both plain material and cell/material printing, offers remarkable advantages and versatility to produce multilateral and cell-laden tissue constructs; however, it has often revealed to be insufficient to fulfill clinical needs. Indeed, three-dimensional (3D) (bio-)printing does not provide one critical element, fundamental to mimic native live tissues, i.e., the ability to change shape/properties with time to respond to microenvironmental stimuli in a personalized manner. This capability is in charge of the so-called “smart materials”; thus, 3D (bio-)printing these biomaterials is a possible way to reach four-dimensional (4D) (bio-)printing. We present a comprehensive review on stimuli-responsive materials to produce scaffolds and constructs via additive manufacturing techniques, aiming to obtain constructs that closely mimic the dynamics of native tissues. Our work deploys the advantages and drawbacks of the mechanisms used to produce stimuli-responsive constructs, using a classification based on the target stimulus: humidity, temperature, electricity, magnetism, light, pH, among others. A deep understanding of biomaterial properties, the scaffolding technologies, and the implant site microenvironment would help the design of innovative devices suitable and valuable for many biomedical applications.

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An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability

Cited by 166 publications

References 157 publications

3D Printing Technologies in Metallic Implants: A Thematic Review on the Techniques and Procedures

3D Printing Technologies in Metallic Implants: A Thematic Review on the Techniques and Procedures

An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multi-material bioprinting

Four-Dimensional (Bio-)printing: A Review on Stimuli-Responsive Mechanisms and Their Biomedical Suitability

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