An overview of applications of CRISPR-Cas technologies in biomedical engineering

Jamehdor, Saleh; Kaboli, Saeed; Naserian, Sina; Omidy, Honey Alef; Teimoori, Ali; Johari, Behrooz; Taromchi, Amir Hossein; Sasano, Yu; Kaboli, Saeed

doi:10.5603/fhc.a2020.0023

Cited by 7 publications

(4 citation statements)

References 111 publications

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“…Initial studies in bacterial cell lines [98,99] and mammalian cells [100][101][102][103][104] have shown that the biologically engineered CRISPR-Cas technology has future potential for correcting gene mutations. Such as malaria blocking genes in mosquitoes [105][106][107][108][109][110], genome editing in zebrafish [111][112][113], removing HIV genes [64,[114][115][116], hepatitis C virus [117] or Parkinson's disease [118]. However, this exciting progress may have unintended consequences and impacts due to 'off-target' effects, which recently are the main limitations of the CRISPR-Cas system because applied genetic corrections can have unpredictable results for future generations.…”

Section: The Immunization and Interference In Prokaryotic Cellsmentioning

confidence: 99%

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

2022

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Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods, soil or water can dramatically improve clinical outcomes and reduce the socioeconomic impact of diseases such as COVID-19. Clustered regularly interspaced short palindromic repeat (CRISPR) and its associated protein Cas12a (previously known as CRISPR-Cpf1) technology is an innovative new-generation genomic engineering tool, also known as ‘genetic scissors’, that has demonstrated the accuracy and has recently been effectively applied as appropriate (E-CRISPR) DNA-sensor to detect the nucleic acid of interest. The CRISPR-Cas12a from Prevotella and Francisella 1 are guided by a short CRISPR RNA (gRNA). The unique simultaneous cis- and trans- DNA cleavage after target sequence recognition at the PAM site, sticky-end (5–7 bp) employment, and ssDNA/dsDNA hybrid cleavage strategies to manipulate the attractive nature of CRISPR–Cas12a are reviewed. DNA-sensors based on the CRISPR-Cas12a technology for rapid, robust, sensitive, inexpensive, and selective detection of virus DNA without additional sample purification, amplification, fluorescent-agent- and/or quencher-labeling are relevant and becoming increasingly important in industrial and medical applications. In addition, CRISPR-Cas12a system shows great potential in the field of E-CRISPR-based bioassay research technologies. Therefore, we are highlighting insights in this research direction. Graphical Abstract

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Section: The Immunization and Interference In Prokaryotic Cellsmentioning

confidence: 99%

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

2022

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“…An RNA-designed (single guide RNA [sgRNA]) for the target point is responsible for endonuclease protein (Cas9[CRISPR-associated protein 9]), Cas12, and Cas13 guidance. By use of the CRISPR toolkit and modified CRISPR toolkit systems, we can knock-in and knock-out genes, transcription activation, and suppression, epigenetic changes, edit the base, and image-specific nucleic acids ( Adli, 2018 ; Jamehdor et al, 2020 ). Research studies have shown that this system is effective in RNA and DNA editing ( Kushawah et al, 2020 ; Ran et al, 2013 ).…”

Section: Main Textmentioning

confidence: 99%

Enhanced High Mutation Rate and Natural Selection to Produce Attenuated Viral Vaccine with CRISPR Toolkit in RNA Viruses especially SARS-CoV-2

Jamehdor

Naserian

Teimoori

2022

Infection, Genetics and Evolution

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The best and most effective way to combat pandemics is to use effective vaccines and live attenuated vaccines are among the most effective vaccines. However, one of the major problems is the length of time it takes to get the attenuated vaccines. Today, the CRISPR toolkit (Clustered Regularly Inerspaced Short Palindromic Repeats) has made it possible to make changes with high efficiency and speed. Using this toolkit to make point mutations on the RNA virus's genome in a coculture of permissive and nonpermissive cells and under controlled conditions can accelerate changes in the genome and accelerate natural selection to obtain live attenuated vaccines.

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“…Some different stem cell types have so far been successfully used in clinical studies and have received scientific approval. Besides viral genome modification, more and more publications are confirming that CRISPR/Cas9 genome editing is a potent technique that can greatly advance biomedicine, such as in virus genome editing and in stem cell research [ 11 , 17 , 18 , 19 ]. Organoids are interesting new systems that help us improve our knowledge of diseases’ mechanisms, development, evolution, homeostasis, and therapy.…”

Section: Introductionmentioning

confidence: 99%

Research and Therapeutic Approaches in Stem Cell Genome Editing by CRISPR Toolkit

et al. 2023

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The most widely used genome editing toolkit is CRISPR (clustered regularly interspaced short palindromic repeats). It provides the possibility of replacing and modifying DNA and RNA nucleotides. Furthermore, with advancements in biological technology, inhibition and activation of the transcription of specific gene(s) has become possible. Bioinformatics tools that target the evolution of CRISPR-associated protein 9 (Cas9) turn this protein into a vehicle that is specific for a DNA or RNA region with single guide RNA (sgRNA). This toolkit could be used by researchers to investigate the function of stem cell gene(s). Here, in this review article, we cover recent developments and applications of this technique in stem cells for research and clinical purposes and discuss different CRISPR/Cas technologies for knock-out, knock-in, activation, or inhibition of gene expression. Additionally, a comparison of several deliveries and off-target detecting strategies is discussed.

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An overview of applications of CRISPR-Cas technologies in biomedical engineering

Cited by 7 publications

References 111 publications

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

Enhanced High Mutation Rate and Natural Selection to Produce Attenuated Viral Vaccine with CRISPR Toolkit in RNA Viruses especially SARS-CoV-2

Research and Therapeutic Approaches in Stem Cell Genome Editing by CRISPR Toolkit

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