An Outbreak of Human Semliki Forest Virus Infections in Central African Republic

Mathiot, C; Grimaud, Gerard; Garry, Philippe; Bouquety, J.C.; Mada, Augustin; Daguisy, Anne Marie; Georges, A. J.

doi:10.4269/ajtmh.1990.42.386

Cited by 102 publications

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“…1 Some of the members of alphaviruses are mild pathogens in humans and some epidemics with flu-like symptoms have been described in central Africa. 2 The life cycle of alphaviruses includes infection through recognition of cell surface receptors such as heparin sulfate and laminin receptors and uptake of viral particles through either fusion with the cell membrane or endocytosis (Figure 1). After the release of the positive-strand RNA genome in the cytoplasm, immediate translation of the viral nonstructural proteins (nsP1-4) occurs leading to the formation of the replicase complex responsible for highly efficient RNA replication.…”

Section: Introductionmentioning

confidence: 99%

Biology and application of alphaviruses in gene therapy

Lundström¹

2005

Gene Ther

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Section: Introductionmentioning

confidence: 99%

Biology and application of alphaviruses in gene therapy

Lundström¹

2005

Gene Ther

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“…The few reported cases of human disease have involved headache, arthralgia and myalgia, presentations that are commonly associated with other alphavirus disease manifestation. In 1987 an outbreak of SFV was recorded in the Central African Republic with severe and prolonged myalgia as a major clinical symptom (Mathiot et al, 1990).…”

Section: Other Alphavirusesmentioning

confidence: 99%

Arthrogenic Alphaviruses and Inflammatory Myopathies

Herrero¹,

Mahalingam²

2011

Idiopathic Inflammatory Myopathies - Recent Developments

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“…Nonspecific binding was determined in the presence of 1 pM substance P methyl ester. -amino-5'-methlylphenoxy)ethane-N,N,N',N'-tetraacetic acid (Fura-2) assays for substance-P-stimulated Ca2+ mobilization in BHK, COS-7 and CHO cells infected with the SFVneurokinin-I virus stock were carried out 10, 16 and 24 h post-infection according to Eistetter et al [31].…”

Section: Radioligand-binding Assaysmentioning

confidence: 99%

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

Lundström¹,

Mills²,

Buell³

et al. 1994

European Journal of Biochemistry

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Human neurokinin-1 receptor cDNA was introduced into the pSFVl Semliki Forest virus (SFV) vector and the in vitro transcribed RNA was electroporated into BHK cells with pSFV-Helper RNA. This procedure resulted in the packaging of a high-titer SFV-NK-1 virus stock containing approximately 5 X lo9 infective units/ml. Infection of baby hamster kidney, COS-7, Chinese hamster ovary and human osteosarcoma cells yielded high levels of human neurokinin-1 receptor expression as assessed by [3H]~ub~tance P binding. The maximal receptor expression level obtained was 4X106 receptors/cell and studies of the post-infection time indicated that a high level of receptor expression was observed 10-24 h post-infection. The human neurokinin-1 receptor expressed in infected baby hamster kidney, COS-7 and Chinese hamster ovary cells was able to stimulate Ca2+ mobilization indicating functional coupling to guanine-nucleotide-binding proteins. The application of the Semliki Forest virus expression system will permit the rapid and efficient production of large quantities of receptor protein for both pharmacological and structural studies. [8, 91. Instrumental in our current understanding of the structure and function of this receptor has been its molecular cloning from a number of different species [2, 10, 111 and many reports have appeared on the membrane topology, ligand binding and interaction with G proteins [12-151 of this protein. However, further progress is being hampered by the lack of large quantitities of purified receptor for structural studies. Moreover, in order to elucidate in more detail the structure/function relationships, receptor mutants will need to be investigated. Earlier strategies for testing seven-transmembrane receptor function have included injection of in vitm synthesized mRNA into Xenopus laevis oocytes [16, 171, expression in bacteria [18, 191 and yeast [20, 211, and Correspondence to K. Lundstrom, Department of Molecular BiFax: +41 22 7946965. Abbreviations. BHK, baby hamster kidney; CHO, Chinese hamster ovary; DMEM, Dulbecco's modified Eagle's medium; Fura-2,

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An Outbreak of Human Semliki Forest Virus Infections in Central African Republic

Cited by 102 publications

References 0 publications

Biology and application of alphaviruses in gene therapy

Biology and application of alphaviruses in gene therapy

Arthrogenic Alphaviruses and Inflammatory Myopathies

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

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