An organogenic plant regeneration system for common bean (Phaseolus vulgaris L.)

Delgado‐Sánchez, Pablo; Saucedo-Ruiz, M.; Guzmán-Maldonado, Salvador Horacio; Villordo-Pineda, Emiliano; González-Chavira, Mario Martín; Fraire-Velázquez, Saúl; Acosta-Gallegos, Jorge Alberto; Mora-Aviles, A.

doi:10.1016/j.plantsci.2005.11.015

Cited by 32 publications

(38 citation statements)

References 21 publications

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“…In contrast to our results, Delgado-Sanchez et al (2006) reported that the application of AS at different concentrations did not produce a relevant increase in bud cluster formation in two Mexican common bean cultivars. Such divergent or contrasting results could be due to differences in cultivars, physiological state of explant and the season of explant collection.…”

Section: Discussioncontrasting

confidence: 99%

“…(Sharma and Rajam, 1995). Our results confirmed earlier observations that the genotype influences the regeneration process via organogenesis in the bean (Santalla et al 1998;Delgado-Sanchez et al 2006;Varisai Mohamed et al 2006). The genotype effects suggest that genetic factors are important in the response to in vitro tissue culture (Santalla et al 1998).…”

Section: Discussionsupporting

confidence: 92%

“…In this sense, Delgado-Sanchez et al (2006) reported that low BAP concentration (0 and 0.1 mgl -1 ) induced only root formation and stem elongation.…”

Section: Discussionmentioning

confidence: 96%

“…Successful acclimatization of common bean in reproduction modes could be altered (Veltcheva et system that results in efficient differentiation, shoot development and whole plant regeneration is an essential requirement for improvement of common bean through genetic transformation or mutagenesis (Svetleva et al 2003;Varisai Mohamed et al 2006). In addition to genetic improvement, in vitro culture is an important tool for the recovery, conservation of germplasm and embryo rescue (Delgado-Sanchez et al 2006).…”

mentioning

confidence: 99%

“…In vitro plant regeneration of Phaseolus has been reported by organogenesis (Malik and Saxena, 1991;Malik and Saxena, 1992;Ahmed et al 2002;Delgado-Sanchez et al 2006;Varisai Mohamed et al 2006) or through somatic embryogenesis (Zambre et al 1998;Schryer et al 2005). Although several protocols have been described in the literature for bean regeneration, development of an optimal in vitro culture system still remains a major challenge since this and other species from the Phaseolusgenus, are recalcitrant for in vitro regeneration .…”

mentioning

confidence: 99%

See 4 more Smart Citations

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Gatica-Arias¹,

Valverde²,

Fonseca³

et al. 2010

Electro Journal of Biotech

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

“…In this sense, Delgado-Sanchez et al (2006) reported that low BAP concentration (0 and 0.1 mgl -1 ) induced only root formation and stem elongation.…”

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Gatica-Arias¹,

Valverde²,

Fonseca³

et al. 2010

Electro Journal of Biotech

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Common Bean

Aragão

Faria²,

Peloso³

et al. 2008

Compendium of Transgenic Crop Plants

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Common bean ( Phaseolus vulgaris ) is an important source of protein in the diet of more than 300 million people throughout the world. Studies on the domestication and organization processes of the common bean genetic variability was first based on morphological traits and biochemical markers and over the last years, several research groups have made efforts in the development of microsatellite markers for common bean, those are considered the most informative class of markers. Early efforts to transform common beans demonstrated its susceptibility to Agrobacterium, and some transgenic tissues or organs, such as calli, leaves, meristems, cotyledon, and hypocotyl have been achieved. In 1993, it was the first reported P. vulgaris transformation, presenting molecular evidences of transgenic progeny. Recently, reproducible systems, based on Agrobacterium and biolistics process, have been developed to achieve transgenic bean plants routinely. Using these systems, some agronomically important genes have been introduced into common bean, such as: the methionine‐rich 2S albumin gene from Brazil nut, bar gene that confers resistance to glufosinate ammonium, LEA protein gene, and genes for resistance to the Bean golden mosaic virus (BGMV). Herbicide and BGMV‐resistant plants were the first transgenic common bean plants to be evaluated under field conditions.

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Somatic Embryogenesis in Common BeanPhaseolus vulgaris L.

Cabrera-Ponce

González-Gómez

León-Ramírez

et al. 2018

Plant Cell Culture Protocols

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Common bean Phaseolus vulgaris L. has been shown to be a recalcitrant plant to induce somatic embryogenesis (SE) under in vitro conditions. An alternative strategy to yield SE is based upon the use of a cytokinin (benzyladenine) coupled with osmotic stress adaptation instead of the auxin-inducing SE in common bean. Here we described the induction of proembryogenic masses (PEM) derived from the apical meristem and cotyledonary zone of zygotic embryos, from which secondary SE indirect embryogenesis emerged. Maturation of SE was achieved by using osmotic stress medium and converted to plants. Long-term recurrent SE was demonstrated by propagation of PEM at early stages of SE. This protocol is currently being applied for stable genetic transformation by means of Agrobacterium tumefaciens and biobalistics as well as basic biochemical and molecular biology research.

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An organogenic plant regeneration system for common bean (Phaseolus vulgaris L.)

Cited by 32 publications

References 21 publications

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Common Bean

Somatic Embryogenesis in Common BeanPhaseolus vulgaris L.

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