An oral ratiometric NIR-II fluorescent probe for reliable monitoring of gastrointestinal diseases in vivo

Li, Tuanwei; Cao, Kaili; Yang, Xiaohu; Liu, Yansheng; Wang, Xingyu; Wu, Feng; Chen, Guangcun; Wang, Qiangbin

doi:10.1016/j.biomaterials.2022.121956

Cited by 21 publications

(5 citation statements)

References 54 publications

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“…After oral delivery to the diseased intestinal tissue, LC-1250 NP presented strong fluorescence emission at 1045 nm while maintaining constant fluorescence intensity at 1250 nm due to the emission tail. Good performance makes LC-1250 NP which is an oral ratiometric NIR-II fluorescent probe a good choice for reliable monitoring of intestinal inflammation in vivo …”

Section: Inflammation In the Bodymentioning

confidence: 99%

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Fluorescence Imaging of Inflammation with Optical Probes

Zhou

Chen

et al. 2023

Chemical & Biomedical Imaging

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Inflammation plays an important role in the occurrence and development of disease; dysregulation of inflammatory progression often leads to disease such as tissue sclerosis, cancers, stroke, etc. Optical imaging technology, due to its higher sensitivity and resolution, can provide finer images for the observation of inflammation. Many optical probes have been developed as contrast agents for optical imaging techniques in different diseases. In this review, we summarize the recent advances of optical probe and imaging methods for imaging inflammation in different organs, such as brain, liver, lung, kidney, intestine, etc. Finally, we discuss the opportunities and challenges of optical probes used in the clinic for inflammation monitoring and prospect their future development in disease detection.

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Section: Inflammation In the Bodymentioning

confidence: 99%

“…The fluorescence ratio of the intestinal tract in the DSS group was enhanced significantly indicating the up-regulated H 2 O 2 level in IBD mice. Panels c and d reproduced with permission from ref . Copyright 2023 Elsevier.…”

Section: Inflammation In the Bodymentioning

confidence: 99%

Fluorescence Imaging of Inflammation with Optical Probes

Zhou

Chen

et al. 2023

Chemical & Biomedical Imaging

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“…In recent years, in vivo imaging within the NIR-II has emerged as a method that benefits from ultralow light scattering and deeper penetration through biological tissues 34,35 . Various NIR-II fluorescent probes have been developed, including organic dyes 36,37 , gold nanoclusters 38 , quantum dots and lanthanide nanocrystals [39][40][41] . However, they often suffer from limited biocompatibility due to the presence of toxic transition metals (e.g., Pb, Cd) or limited stability due to photobleaching 42,43 .…”

mentioning

confidence: 99%

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Settele,

Schrage,

Jung

et al. 2024

Nat Commun

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Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors.

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“…Therefore, there is an urgent need to develop rapid and sensitive tools to detect H 2 O 2 to accurately identify the various states of chronic wound healing . Recently, fluorescent probes have been considered as powerful tools to monitor biological active species and processes due to their noninvasiveness, high sensitivity, and excellent spatial and temporal resolution. − Some fluorescent probes have been reported for monitoring H 2 O 2 in living cells and in vivo through the careful design; however, the probes for monitoring the concentration fluctuation during the chronic wound healing process are still challenging. − Moreover, compared to the fluorescent emission in the visible light region, fluorescent probes with excitation/emission wavelength in the near-infrared (NIR) region get benefit from the low background interference, small light damage and deep penetration, which are more suitable for bioimaging. − With this in mind, we attempt to develop a NIR fluorescent probe for rapid and highly sensitive detection of H 2 O 2 in living cells and in vivo to track the concentration fluctuations of H 2 O 2 during chronic wound processes.…”

mentioning

confidence: 99%

Near-Infrared Fluorescence Probe for Indication of the Pathological Stages of Wound Healing Process and Its Clinical Application

Luo,

Cheng,

Zhang

et al. 2024

ACS Sens.

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Chronic wound healing is one of the most complicated biological processes in human life, which is also a serious challenge for human health. During the healing process, multiple biological pathways are activated, and various kinds of reactive oxygen species participate in this process. Hydrogen peroxide (H 2 O 2 ) involves in chronic wounds and its concentration is fluctuated in different pathological stages during the wound healing process. Therefore, H 2 O 2 may be recognized as a powerful biomarker to indicate the wound healing process. However, the pathological roles of H 2 O 2 cannot be fully understood yet. Herein, we proposed a near-infrared fluorescent probe DCM-H 2 O 2 for highly sensitive and rapid detection of H 2 O 2 in living cells and scald and incision wound mice models. DCM-H 2 O 2 exhibited a low detection limit and high specificity with low cytotoxicity for H 2 O 2 , which had great potential for its application in vivo. The probe was successfully utilized to monitor the fluctuation of endogenous H 2 O 2 in the proliferation process of human immortalized epidermal (HACAT) cells, which confirmed that H 2 O 2 participated in the cells' proliferation activity through a growth factor signaling pathway. In the scald and incision wound mice models, H 2 O 2 concentration fluctuations at different pathological stages during the wound healing process could be obtained by in vivo fluorescence imaging. Finally, H 2 O 2 concentrations in different stages of human diabetic foot tissues were also confirmed by the proposed probe. We expect that H 2 O 2 could be a sensitive biomarker to indicate the wound healing process.

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An oral ratiometric NIR-II fluorescent probe for reliable monitoring of gastrointestinal diseases in vivo

Cited by 21 publications

References 54 publications

Fluorescence Imaging of Inflammation with Optical Probes

Fluorescence Imaging of Inflammation with Optical Probes

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Near-Infrared Fluorescence Probe for Indication of the Pathological Stages of Wound Healing Process and Its Clinical Application

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