volume 1, issue 3, P100110 2020
DOI: 10.1016/j.xpro.2020.100110
View full text
Sign up to set email alerts

Abstract: Summary Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system. One major challenge in using this technique is to obtain RNA without compromising its integrity. Here, we present a protocol optimized for radish root tissue sections using Steedman's wax embedding to obtain high-quality RNA suitable for next-generation sequencing analysis. For complete details on the use and…

Expand abstract