volume 1, issue 1, P100020 2020
DOI: 10.1016/j.xpro.2020.100020
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Abstract: Summary Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function. A common method to study protein-DNA interactions is chromatin immunoprecipitation (ChIP). Here, we present a revised ChIP protocol to determine protein-DNA interactions for the yeast Saccharomyces cerevisiae . We optimized several aspects of the procedure, including cross-linking and …

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