An LC-MS/MS analysis for seven sex hormones in serum

Yuan, Tengfei; Le, Juan; Cui, Yan; Peng, Rui; Wang, Shao-Ting; Li, Yan

doi:10.1016/j.jpba.2018.09.014

Cited by 39 publications

(22 citation statements)

References 23 publications

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“…The variability in the precision and accuracy of different mass spectrometry methods (seven high performance liquid chromatography tandem mass spectrometry assays, and one gas chromatography tandem mass spectrometry assays) has been reported, and the results of the comparison of eight mass spectrometry methods showed that the within-run variability at 297 and 8.47 ng/dl ranged between 1.40-11.36% CV and 2.52-25.58% CV, respectively (13). Sample preparation includes several stages, such as derivatization procedure, liquid-liquid extraction (LLE) and solid phase extraction, which has been indicated to result in the poor sensitivity and low extraction efficiency of the different methods (14)(15)(16). Therefore, certain mass spectrometry methods for testosterone determination cannot meet the clinical requirements.…”

Section: Quantitative Determination Of Human Serum Testosterone Via Imentioning

confidence: 99%

Quantitative determination of human serum testosterone via isotope dilution ultra‑performance liquid chromatography tandem mass spectrometry

Sun

Xue

et al. 2020

Mol Med Rep

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Quantification of testosterone serves an important role in the differential diagnosis of androgen-related endocrine diseases. Mass spectrometry exhibits higher accuracy and lower variability than immunoassays, especially at low testosterone concentrations. The present study developed and validated an isotope dilution ultra-performance liquid chromatography tandem mass spectrometry method for determination of human serum testosterone. The serum was equilibrated with an isotopic internal standard and treated with acidic buffer to release hormones from their binding proteins. Testosterone was extracted via two serial liquid-liquid extractions. In the first stage, the lipid fractions from an acidic buffer solution were isolated using ethyl acetate and n-hexane. The organic phase was evaporated and reconstituted in a basic buffer solution. In the second stage, the polar impurities of n-hexane extraction were removed. Total testosterone in serum was quantified via ultra-performance liquid chromatography tandem mass spectrometry in multiple reaction monitoring mode with positive electrospray ionization. The coefficient of variation of the method for intra-and inter-assay was 2.13% (1.40-2.77%) and 3.44% (3.06-3.66%), respectively. The recovery ranged from 94.32 to 108.60% for different samples. The limit of detection was 0.50 ng/dl and the linear range was from 1.00 to 1,000.00 ng/dl. In addition, the extraction efficiency in three different levels of quality control of the serum ranged from 85.02 to 93.29%. Moreover, structural analogues were investigated and were not indicated to affect the quantification of testosterone. The present method may enable quantification of testosterone in a clinical setting with high precision and accuracy.

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Section: Quantitative Determination Of Human Serum Testosterone Via Imentioning

confidence: 99%

Quantitative determination of human serum testosterone via isotope dilution ultra‑performance liquid chromatography tandem mass spectrometry

Sun

Xue

et al. 2020

Mol Med Rep

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“…Quantification of testosterone in biological samples has many clinical and research applications, including hormone profiling in animals (Farke, Rattenberger, Roiger, & Meyer, 2011;Hauser, Deschner, & Boesch, 2008;Holst, Kushnir, & Bergquist, 2015;Kaabia, Laparre, Cesbron, Le Bizec, & Dervilly-Pinel, 2018;Kaufmann, Butcher, Maden, Walker, & Widmer, 2019;Legacki, Robeck, Steinman, & Conley, 2020;Shackleton, 2010), hormone profiling in humans (Häkkinen et al, 2018;Zheng, Zhao, Zhu, & Cai, 2019) and disease investigation (Kushnir et al, 2009;Lood et al, 2018;Yuan et al, 2019). Being able to accurately measure testosterone concentrations in human samples is essential in accurate diagnoses of hypogonadism, polycystic ovary syndrome, and certain cancers (Bhasin et al, 2010;Ka, 2016;Loblaw et al, 2007;Wierman et al, 2006).…”

Section: Testosterone Bioanalysismentioning

confidence: 99%

“…Sample volumes ranged from 100 to 500 μg/ml as summarized in Harwood, & Handelsman, 2015;Legacki et al, 2020;Matysik & Liebisch, 2017;Yuan et al, 2019), but is also applied to extractions from plasma (Desai et al, 2019). Other serum relevant solvents include toluene (Häkkinen et al, 2018), 1-chlorobutane (Ke, Bertin, Gonthier, Simard, & Labrie, 2014), dichloromethane (Qin et al, 2020) and di-isopropyl ether (Star-Weinstock, Williamson, Dey, Pillai, & Purkayastha, 2012).…”

Section: Testosterone Analysis From Serum and Plasmamentioning

confidence: 99%

“…LLE relies on the partition of the analyte from the aqueous matrix into an immiscible organic solvent. Methyl tert ‐butyl ether (MTBE) is the most common LLE solvent used to extract testosterone from serum (Handelsman, Desai, Seibel, Le Couteur, & Cumming, 2020; Keski‐Rahkonen, Huhtinen, Poutanen, & Auriola, 2011; Keski‐Rahkonen, Desai, Jimenez, Harwood, & Handelsman, 2015; Legacki et al, 2020; Matysik & Liebisch, 2017; Yuan et al, 2019), but is also applied to extractions from plasma (Desai et al, 2019). Other serum relevant solvents include toluene (Häkkinen et al, 2018), 1‐chlorobutane (Ke, Bertin, Gonthier, Simard, & Labrie, 2014), dichloromethane (Qin et al, 2020) and di‐isopropyl ether (Star‐Weinstock, Williamson, Dey, Pillai, & Purkayastha, 2012).…”

Section: Matrices and Sample Preparationmentioning

confidence: 99%

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Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

Gravitte

Archibald

Cobble

et al. 2020

Biomedical Chromatography

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Liquid chromatography, coupled with tandem mass spectrometry, presents a powerful tool for the quantification of the sex steroid hormones 17-β estradiol, progesterone and testosterone from biological matrices. The importance of accurate quantification with these hormones, even at endogenous levels, has evolved with our understanding of the role these regulators play in human development, fertility and disease risk and manifestation. Routine monitoring of these analytes can be accomplished by immunoassay techniques, which face limitations on specificity and sensitivity, or using gas chromatography-mass spectrometry. LC-MS/MS is growing in capability and acceptance for clinically relevant quantification of sex steroid hormones in biological matrices and is able to overcome many of the limitations of immunoassays. Analyte specificity has improved through the use of novel derivatizing agents, and sensitivity has been refined through the use of high-resolution chromatography and mass spectrometric technology. This review highlights these innovations, among others, in LC-MS/MS steroid hormone analysis captured in the literature over the last decade.

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“…In clinical diagnostics, several detection methods were developed for detecting progesterone, including spectroscopic, spectrometric (hyphenated with chromatography), enzymatic immunoassay, etc [13,14,15,16]. More recently, several examples of electrochemical immunoassay emerged, together with a few cases of direct voltammetric detection performed on mercury, tin and various carbon electrodes in alkaline media [3,4,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Bismuth Film Electrode for Detection of Progesterone

Zidarič¹,

Jovanovski²,

Hočevar³

2018

Sensors

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Progesterone is an important hormone responsible, among others, for maintaining pregnancy via inhibition of uterus muscles activity; thus, following its concentration levels in pregnant women is of immense importance in the endeavor to prevent premature birth. In this work, the nanostructured bismuth film electrode (nsBiFE) was studied for detection of progesterone in neutral medium. Due to the ability to accumulate progesterone at the nsBiFE, the adsorptive cathodic stripping voltammetry was beneficially exploited. The nsBiFE was prepared on the surface of a glassy carbon supporting electrode and several parameters influencing the detection of progesterone were investigated. The nsBiFE exhibited superior electroanalytical characteristics in comparison to other bismuth-based electrodes and unmodified glassy carbon electrode together with satisfactory response toward low concentrations of progesterone, which are consistent with clinically significant levels.

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An LC-MS/MS analysis for seven sex hormones in serum

Cited by 39 publications

References 23 publications

Quantitative determination of human serum testosterone via isotope dilution ultra‑performance liquid chromatography tandem mass spectrometry

Quantitative determination of human serum testosterone via isotope dilution ultra‑performance liquid chromatography tandem mass spectrometry

Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

Nanostructured Bismuth Film Electrode for Detection of Progesterone

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