An investigation of possible models for the production of progressive pulmonary fibrosis in the rat. The effects of repeated intratracheal instillation of bleomycin

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

“…These changes were a likely consequence of the severe morphological alterations occurring in the lung following bleomycin treatment, which manifested as a heterogeneous distribution of dense inflammatory cell infiltrate and accumulations of fibrous masses and bands in the lung parenchyma, coupled with thickening of the alveolar walls, all of which are observations consistent with previous reports [26,27,28]. Likewise, as with previous reports, bleomycin-treated C57BL/6 mice developed pulmonary oedema, deduced from the significant increase in wet lung weights following bleomycin treatment, compared to vehicle control mice [29,30].…”

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

“…These changes were a likely consequence of the severe morphological alterations occurring in the lung following bleomycin treatment, which manifested as a heterogeneous distribution of dense inflammatory cell infiltrate and accumulations of fibrous masses and bands in the lung parenchyma, coupled with thickening of the alveolar walls, all of which are observations consistent with previous reports [26,27,28]. Likewise, as with previous reports, bleomycin-treated C57BL/6 mice developed pulmonary oedema, deduced from the significant increase in wet lung weights following bleomycin treatment, compared to vehicle control mice [29,30].…”

Development of a non-infectious rat model of acute exacerbation of idiopathic pulmonary fibrosis

“…The rat model of AE-IPF in the current study is simpler and safer than the animal models of AE-IPF induced by infection, and shows satisfactory reproducibility. Previous studies that used repetitive intratracheal perfusion with BLM have shown that consecutive weekly intratracheal instillations of BLM result in severe pulmonary fibrosis (18,29). In the rat model of the current study, the ALI induced by the second perfusion was associated with lung fibrosis, and this fibrosisrelated ALI mimics human AE-IPF.…”

“…Animal models of BLM-induced pulmonary fibrosis have been widely used to study IPF (10-12). Brown et al have shown that repetitive intratracheal perfusion with BLM worsens the BLM-induced lung injury and fibrosis in rats (18). Based on their study, we developed a rat AE-IPF The relative mRAN level of NF-κB was significantly higher in the BLM + BLM group than in the BLM group at all of the three time points.…”

Anti-Inflammatory Effects of Adult Stem Cells in Sustained Lung Injury: A Comparative Study

“…This second dose would cause further injury on the background of ongoing inflammation and early fibrosis. Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25].…”

“…Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25]. Furthermore, Pinart et al has also shown that a double dose of bleomycin mimics the mechanical dysfunction of the lung as occurs in chronic human interstitial lung disease [14].…”

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

“…These changes were a likely consequence of the severe morphological alterations occurring in the lung following bleomycin treatment, which manifested as a heterogeneous distribution of dense inflammatory cell infiltrate and accumulations of fibrous masses and bands in the lung parenchyma, coupled with thickening of the alveolar walls, all of which are observations consistent with previous reports [26,27,28]. Likewise, as with previous reports, bleomycin-treated C57BL/6 mice developed pulmonary oedema, deduced from the significant increase in wet lung weights following bleomycin treatment, compared to vehicle control mice [29,30].…”

Development of a non-infectious rat model of acute exacerbation of idiopathic pulmonary fibrosis

“…The rat model of AE-IPF in the current study is simpler and safer than the animal models of AE-IPF induced by infection, and shows satisfactory reproducibility. Previous studies that used repetitive intratracheal perfusion with BLM have shown that consecutive weekly intratracheal instillations of BLM result in severe pulmonary fibrosis (18,29). In the rat model of the current study, the ALI induced by the second perfusion was associated with lung fibrosis, and this fibrosisrelated ALI mimics human AE-IPF.…”

“…Animal models of BLM-induced pulmonary fibrosis have been widely used to study IPF (10-12). Brown et al have shown that repetitive intratracheal perfusion with BLM worsens the BLM-induced lung injury and fibrosis in rats (18). Based on their study, we developed a rat AE-IPF The relative mRAN level of NF-κB was significantly higher in the BLM + BLM group than in the BLM group at all of the three time points.…”

Anti-Inflammatory Effects of Adult Stem Cells in Sustained Lung Injury: A Comparative Study

“…This second dose would cause further injury on the background of ongoing inflammation and early fibrosis. Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25].…”

“…Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25]. Furthermore, Pinart et al has also shown that a double dose of bleomycin mimics the mechanical dysfunction of the lung as occurs in chronic human interstitial lung disease [14].…”

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

“…These changes were a likely consequence of the severe morphological alterations occurring in the lung following bleomycin treatment, which manifested as a heterogeneous distribution of dense inflammatory cell infiltrate and accumulations of fibrous masses and bands in the lung parenchyma, coupled with thickening of the alveolar walls, all of which are observations consistent with previous reports [26,27,28]. Likewise, as with previous reports, bleomycin-treated C57BL/6 mice developed pulmonary oedema, deduced from the significant increase in wet lung weights following bleomycin treatment, compared to vehicle control mice [29,30].…”

Development of a non-infectious rat model of acute exacerbation of idiopathic pulmonary fibrosis

“…The rat model of AE-IPF in the current study is simpler and safer than the animal models of AE-IPF induced by infection, and shows satisfactory reproducibility. Previous studies that used repetitive intratracheal perfusion with BLM have shown that consecutive weekly intratracheal instillations of BLM result in severe pulmonary fibrosis (18,29). In the rat model of the current study, the ALI induced by the second perfusion was associated with lung fibrosis, and this fibrosisrelated ALI mimics human AE-IPF.…”

“…Animal models of BLM-induced pulmonary fibrosis have been widely used to study IPF (10-12). Brown et al have shown that repetitive intratracheal perfusion with BLM worsens the BLM-induced lung injury and fibrosis in rats (18). Based on their study, we developed a rat AE-IPF The relative mRAN level of NF-κB was significantly higher in the BLM + BLM group than in the BLM group at all of the three time points.…”

Anti-Inflammatory Effects of Adult Stem Cells in Sustained Lung Injury: A Comparative Study

“…This second dose would cause further injury on the background of ongoing inflammation and early fibrosis. Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25].…”

“…Brown et al demonstrated that lymphocytes and polymorphonuclear cells were present at 28 days and persistent macrophage infiltration up to 60 days following 2 doses of bleomycin [25]. In addition they showed pulmonary oedema was present at 28 days and that there was persistence of fibrosis at 90 days after the second dose of bleomycin [25]. Furthermore, Pinart et al has also shown that a double dose of bleomycin mimics the mechanical dysfunction of the lung as occurs in chronic human interstitial lung disease [14].…”