An investigation of circadian rhythm in<i>Escherichia coli</i>

Yang, Wen; Zhou, Wei; Zhu, Xinxin; Cheng, Songbai; Xiao, Guoguang; Li, Yiyuan; Zhu, Yu; Wang, Zhengrong; Wan, Chaomin

doi:10.1080/09291016.2015.1052650

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…Some strategies, such as circadian rhythms, require explicit molecular clocks for proper execution (1, 2), but clocks may also be present in nonphotosynthetic prokaryotes (3, 4). Bacteria can also exhibit anticipatory control (5), in which they respond to external cues, such as changes in temperature and oxygen concentration (6), to predict and adapt to environmental change before it occurs.…”

Section: Introductionmentioning

confidence: 99%

Microbial Communities Are Well Adapted to Disturbances in Energy Input

2016

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Within the broader ecological context, biological communities are often viewed as stable and as only experiencing succession or replacement when subject to external perturbations, such as changes in food availability or the introduction of exotic species. Our findings indicate that microbial communities can exhibit strong internal dynamics that may be more important in shaping community succession than external drivers. Dynamic “unstable” communities may be important for ecosystem functional stability, with rare organisms playing an important role in community restructuring. Understanding the mechanisms responsible for internal community dynamics will certainly be required for understanding and manipulating microbiomes in both host-associated and natural ecosystems.

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Section: Introductionmentioning

confidence: 99%

Microbial Communities Are Well Adapted to Disturbances in Energy Input

2016

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“…Microorganisms host a diverse repertoire of temporal strategies to maximize their productivity under a variety of environmental settings that undergo periodic as well as aperiodic change. Some strategies, such as circadian rhythms, require explicit molecular clocks for proper execution (1, 2), but clocks may also be present in non-photosynthetic prokaryotes (3, 4). Bacteria can also exhibit anticipatory control (5) in which they respond to external cues, such as changes in temperature and oxygen concentration (6), to predict and adapt to environmental change before it occurs.…”

Section: Introductionmentioning

confidence: 99%

Microbial Communities are Well Adapted to Disturbances in Energy Input

Fernandez‐Gonzalez

Huber

Vallino

2016

Preprint

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Although microbial systems are well suited for studying concepts in ecological theory, little is known about how microbial communities respond to longterm periodic perturbations beyond diel oscillations. Taking advantage of an ongoing microcosm experiment, we studied how methanotrophic microbial communities adapted to disturbances in energy input over a 20-day cycle period. Sequencing of bacterial 16S rRNA genes together with quantification of microbial abundance and ecosystem function were used to explore the long-term dynamics (510 days) of methanotrophic communities under continuous versus cyclic chemical energy supply. We observed that microbial communities appeared inherently well adapted to disturbances in energy input and that changes in community structure in both treatments were more dependent on internal dynamics than on external forcing. The results also showed that the rare biosphere was critical to seeding the internal community dynamics, perhaps due to cross-feeding or other strategies. We conclude that in our experimental system, internal feedbacks were more important than external drivers in shaping the community dynamics over time, suggesting that ecosystems can maintain their function despite inherently unstable community dynamics. IMPORTANCEWithin the broader ecological context, biological communities are often viewed as stable and as only experiencing succession or replacement when subject to external perturbations, such as changes in food availability or the introduction of exotic species. Our findings indicate that microbial communities can exhibit strong internal dynamics that may be more important in shaping community succession than external drivers. Dynamic "unstable" communities may be important for ecosystem functional stability, with rare organisms playing an important role in community restructuring. Understanding the mechanisms responsible for internal community dynamics will certainly be required for understanding and manipulating microbiomes in both host-associated and natural ecosystems.KEYWORDS: 16S rRNA gene, bacteria, internal community dynamics, microbial community dynamics, chemostat cultures, endogenous drivers, energy input pulse, rare biosphere, structure and function M icroorganisms host a diverse repertoire of temporal strategies to maximize their productivity under a variety of environmental settings that undergo periodic as well as aperiodic change. Some strategies, such as circadian rhythms, require explicit molecular clocks for proper execution (1, 2), but clocks may also be present in nonphotosynthetic prokaryotes (3,4). Bacteria can also exhibit anticipatory control (5), in which they respond to external cues, such as changes in temperature and oxygen concentration (6), to predict and adapt to environmental change before it occurs. Bacteria that anticipate environmental change have an obvious fitness advantage, and anticipatory strategies may stabilize ecosystems against perturbations (7,8). Temporal strategies that do not rely on internal clocks include ...

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“…Afterwards, we transferred the mixtures to 96-well plates and used a microplate reader (Bio-Rad Model 680, US) to detect absorbance at 540nm. 23 DMSO was applied as a control. In each group, three replicates were set.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Pro-Osteogenic Coaxially Electrospun Magnesium Oxide Nanoparticles-Polycaprolactone /Parathyroid Hormone-Polycaprolactone Composite Barrier Membrane for Guided Bone Regeneration

Dong¹,

Yao²,

Cai³

et al. 2023

IJN

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Introduction An antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was fabricated to satisfy the complicated and phased requirements of GBR process. Methods In this study, we synthesize dual-functional coaxially electrospun nanofiber GBR membranes by encapsulating parathyroid hormone (PTH) in the core layer and magnesium oxide nanoparticles (MgONPs) in the shell layer (MgONPs-PCL/PTH-PCL). Herein, the physicochemical characterization of MgONPs-PCL/PTH-PCL, the release rates of MgONPs and PTH, and antibacterial efficiency of the new membrane were evaluated. Furthermore, the pro-osteogenicity of the membranes was assessed both in-vitro and in-vivo. Results We successfully fabricated a coaxially electrospun nanofiber MgONPs-PCL/PTH-PCL membrane with the majority of nanofibers (>65%) ranged from 0.40~0.60μm in diameter. MgONPs-PCL/PTH-PCL showed outstanding antibacterial potential against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) through the release of MgONPs. We also discovered that the incorporation of MgONPs significantly prolonged the release of PTH. Furthermore, both the in-vivo and in-vitro studies demonstrated that high dosage of PTH promoted pro-osteogenicity of the membrane to improve bone regeneration efficacy with the presence of MgONPs. Conclusion The new composite membrane is a promising approach to enhance bone regeneration in periodontitis or peri-implantitis patients with large-volume bone defects.

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An investigation of circadian rhythm inEscherichia coli

Cited by 7 publications

References 26 publications

Microbial Communities Are Well Adapted to Disturbances in Energy Input

Microbial Communities Are Well Adapted to Disturbances in Energy Input

Microbial Communities are Well Adapted to Disturbances in Energy Input

Antimicrobial and Pro-Osteogenic Coaxially Electrospun Magnesium Oxide Nanoparticles-Polycaprolactone /Parathyroid Hormone-Polycaprolactone Composite Barrier Membrane for Guided Bone Regeneration

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