An interlaboratory evaluation of the IFCC method for aspartate aminotransferase with use of purified enzyme materials.

Sampson, Eric J.; Whitner, V S; Burtis, C. A.; McKneally, S S; Fast, Douglas M.; Bayse, D D

doi:10.1093/clinchem/26.8.1156

Cited by 19 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum Liver functions were determined by measuring ALT as described by Hafkenscheid and Dijt (1979) and AST according to Sampson et al (1980) in serum using the enzymatic methods.…”

Section: Evaluation Of Biochemical Parametersmentioning

confidence: 99%

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Mahmoud

El‐kott

AlGwaiz

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

The present study aimed to assess the antioxidative, anti-in ammatory, antiapoptotic, and antidepression impacts of Moringa oleifera Lam. leaf ethanolic extract (MOLE) in the hippocampus and cerebral cortex of CCl 4 -induced mouse model of hepatic encephalopathy. High-performance liquid chromatography was used to detect marker compounds; rutin and β-sitosterol. Animals were divided into four groups; vehicle group, CCl 4 treated group, MOLE treated group, and (CCl 4 + MOLE) group treated with MOLE for 14 days before CCl 4 -induced neurotoxicity. MOLE decreased alanine aminotransferase, aspartate aminotransferase, corticosterone, and ammonia levels in serum and improved the antioxidant status of CCl 4 treated mice in the hippocampus and cerebral cortex. It reduced the expression of toll-like receptor (TLR)4, TLR2, myeloid differentiation primary response 88 (MYD88), and nuclear factor-kappa B (NF-κB) genes and the protein levels of the pro-inflammatory cytokines. MOLE also attenuated apoptosis, as revealed by the reduced expression of caspase3, and prevented histological deterioration. Furthermore, MOLE attenuated CCl 4 -induced anxiety and depression-like behavioral changes. Collectively, MOLE modulates neuroin ammation, oxidative stress, TLR4/2-MyD88/NF-κB signaling, and apoptosis in the hippocampus and cerebral cortex of the hepatic encephalopathy experimental model.

show abstract

Section: Evaluation Of Biochemical Parametersmentioning

confidence: 99%

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Mahmoud

El‐kott

AlGwaiz

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…Although serum levels of ALT and AST can be used to diagnose liver disease, they are less specific markers for this condition, as high AST levels, in particular, are seen following skeletal muscle, brain, heart, and red blood cells damage (Pirmadah et al, 2020; Pratt & Kaplan, 2000). Thus, serum ALT is a better indicator of liver disease due to its higher and more specific expression in hepatic cells (Ruhl & Everhart, 2009; Sampson et al, 1980; Weibrecht, Dayno, Darling, & Bird, 2010). Epidemiological studies have shown an association between these enzymes and the risk of type 2 diabetes (Kunutsor, Apekey, & Walley, 2013), cardiovascular disease (Kunutsor, Apekey, & Khan, 2014), and mortality (Kunutsor, Apekey, Seddoh, & Walley, 2014).…”

Section: Introductionmentioning

confidence: 99%

What is the influence of cinnamon supplementation on liver enzymes? A systematic review and meta‐analysis of randomized controlled trials

Mousavi

Jayedi

Bagheri

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

Existing evidence has uncovered the potential health benefits of cinnamon intake; however, its effect on liver function is unclear. Thus, the aim of this systematic review and meta-analysis was to examine the effect of cinnamon supplementation on liver enzymes. Relevant articles were identified through a systematic search in PubMed/Medline, Scopus, Web of Science, Cochrane Library, and Embase up to September 2020. All trials assessing the effect of oral cinnamon supplementation on serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in adults were included. The pooled effect sizes were obtained using the random-effects model and expressed as mean difference (MD) and 95% confidence intervals (CI). A total of seven original trials (nine treatment arms) involving a total of 256 subjects were included in the final analysis. The pooled analysis indicated that cinnamon supplementation had no significant effect on serum levels of ALT, AST, and ALP. However, there was a significant reduction in ALT levels in patients with type 2 diabetes (MD: À4.01 U/L; 95% CI: À6.86, À1.15) and in trials with low-dose supplementation (<1,500 mg/d), follow-up duration longer than 12 weeks, and in the elderly patients (aged>50 years). The beneficial effects of cinnamon intake were also shown in AST levels in patients with type 2 diabetes and trials with long-term follow-up (>12 weeks). Longer-term, oral cinnamon supplementation may improve serum levels of liver enzymes in patients with type 2 diabetes. Further high-quality studies are needed, especially in populations with abnormal liver enzyme levels, to firmly establish the clinical efficacy of cinnamon on liver function.

show abstract

“…AST, which appears in skeletal muscle, the brain, the heart and red blood cells, is less specific for liver damage (6,7). The concentration of ALT is found to be lower than AST in all cells, except in hepatic cells; therefore, its elevation is particularly related to liver disease (8)(9)(10). Gammaglutamyl transpeptidase (GGTP) is another nonspecific marker of liver disease and also found in other organs; it is a plasma membrane enzyme that can counteract oxidative stress by increasing intracellular glutathione synthesis (11,12).…”

Section: Introductionmentioning

confidence: 99%

Does l-carnitine supplementation affect serum levels of enzymes mainly produced by liver? A systematic review and meta-analysis of randomized controlled clinical trials

et al. 2019

View full text Add to dashboard Cite

show abstract

An interlaboratory evaluation of the IFCC method for aspartate aminotransferase with use of purified enzyme materials.

Cited by 19 publications

References 0 publications

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

What is the influence of cinnamon supplementation on liver enzymes? A systematic review and meta‐analysis of randomized controlled trials

Does l-carnitine supplementation affect serum levels of enzymes mainly produced by liver? A systematic review and meta-analysis of randomized controlled clinical trials

Contact Info

Product

Resources

About