An integrated strategy for chemical, biological and palynological standardization of bee propolis

Nada, Ahmed A.; Nour, Iman H.; Metwally, Aly M.; Asaad, Aya M.; Eldin, Safa M. Shams; Ibrahim, Reham S.

doi:10.1016/j.microc.2022.107923

Cited by 4 publications

(5 citation statements)

References 35 publications

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“…On a chemical level, a fusion of resins along with wax, essential oils, balsams, phenolic compounds such as flavonoids, aromatic acids and their esters, and pollen grains amidst other constituents make up the concoction that is propolis [ 15 ]. The exact fabric of propolis is inconsistent and varies greatly between nonidentical samples [ 16 ] based on numerous factors including: available surrounding vegetation, climate, collection time [ 17 ], and the cultivating bee’s subspecies [ 18 ]. To further illustrate this point, European propolis has been demonstrated to contain phenolics as flavonoid agylcones, phenolic acids and their corresponding esters.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

Nada,

Metwally,

Asaad

et al. 2024

BMC Complement Med Ther

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Background Type 2 Diabetes mellitus (DM) is an affliction impacting the quality of life of millions of people worldwide. An approach used in the management of Type 2 DM involves the use of the carbohydrate-hydrolyzing enzyme inhibitor, acarbose. Although acarbose has long been the go-to drug in this key approach, it has become apparent that its side effects negatively impact patient adherence and subsequently, therapeutic outcomes. Similar to acarbose in its mechanism of action, bee propolis, a unique natural adhesive biomass consisting of biologically active metabolites, has been found to have antidiabetic potential through its inhibition of α-amylase. To minimize the need for ultimately novel agents while simultaneously aiming to decrease the side effects of acarbose and enhance its efficacy, combination drug therapy has become a promising pharmacotherapeutic strategy and a focal point of this study. Methods Computer-aided molecular docking and molecular dynamics (MD) simulations accompanied by in vitro testing were used to mine novel, pharmacologically active chemical entities from Egyptian propolis to combat Type 2 DM. Glide docking was utilized for a structure-based virtual screening of the largest in-house library of Egyptian propolis metabolites gathered from literature, in addition to GC–MS analysis of the propolis sample under investigation. Thereafter, combination analysis by means of fixed-ratio combinations of acarbose with propolis and the top chosen propolis-derived phytoligand was implemented. Results Aucubin, identified for the first time in propolis worldwide and kaempferol were the most promising virtual hits. Subsequent in vitro α-amylase inhibitory assay demonstrated the ability of these hits to significantly inhibit the enzyme in a dose-dependent manner with an IC50 of 2.37 ± 0.02 mM and 4.84 ± 0.14 mM, respectively. The binary combination of acarbose with each of propolis and kaempferol displayed maximal synergy at lower effect levels. Molecular docking and MD simulations revealed a cooperative binding mode between kaempferol and acarbose within the active site. Conclusion The suggested strategy seems imperative to ensure a steady supply of new therapeutic entities sourced from Egyptian propolis to regress the development of DM. Further pharmacological in vivo investigations are required to confirm the potent antidiabetic potential of the studied combination.

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Section: Introductionmentioning

confidence: 99%

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

Nada,

Metwally,

Asaad

et al. 2024

BMC Complement Med Ther

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“…Propolis is a natural resinous waxy bee product, obtain collecting from plants' (trees, flowers, buds and leaves exudates) secretions and mixed up bee wax by bees (Silva et al 2012;Silva et al 2013, Nada et al 2022, Ali et al 2023. Propolis gains strong and adhesive feature due to the modification of the resinous structure by bees (Silici 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Propolis could be categorise according to geographical and botanical origin, climatic conditions, the genetic factors of bee, physical and chemical properties (Nada et al 2022). The melissopalynological analyses of propolis provides determining the botanical origin.…”

Section: Introductionmentioning

confidence: 99%

“…Because the propolis biochemical content is directly related with plants that visited by bees. Either our country or around the world there are many melissopalynological researches on propolis (Park et al 2002;Gencay Çelemli and Sorkun 2012;Popova et al 2013;Bayram 2015;Kızılpınar et al 2017;Tatlısulu and Özgör 2023). This preliminary study was carried out for contribute the former researches on this subject and lead beekeepers to choose valid areas to place their beehives.…”

Section: Introductionmentioning

confidence: 99%

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Palynological Analyses of Malatya Propolis Samples

Saatçıoğlu¹,

KARLIDAĞ²

2023

Manas Journal of Agriculture Veterinary and Life Sciences

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Propolis is a sticky resinous mixture of plant secretions and bee wax. Propolis has used for ethnobotanical reasons from ancient cultures and nowadays the researchers focus on testing propolis extracts and raw forms for health problems. Knowledge about the quality of propolis samples is very important to solve various health problems. The quality of propolis samples highly associated with botanical origin. This preliminary study was conducted for determining the botanical origin of Malatya propolis samples in 2022. For palynological research, propolis samples were prepared to microscopic analyses. The dominant taxa was listed according to districts as; for Akcadag Carduus (9.2 %), Astragalus (7.4 %), Onobrychis (7.1 %), Xanthium (6.8 %), Verbascum (6.1 %), Taraxacum (5.3 %); for Battalgazi Poaceae (28.2 %), Carduus (7.7 %), Astragalus (5.9 %); for Darende Onobrychis (11.9 %), Verbascum (10.5 %), Carduus (8.7 %), Poaceae e (5.8 %), for Hekimhan Carduus (9.3 %), Poaceae (8.9 %), Astragalus (6.8 %), Verbascum (6.1 %) Xanthium (5.4 %), for Puturge Onobrychis (14.1 %), Astragalus (8.8 %), Poaceae (8.7 %), Carduus (5.1 %), Cistus (5.1 %), for Yesilyurt Poaceae (10.7 %), Astragalus (6.1 %), Carduus (5.8 %), Verbascum (5.5 %). The aim of this study to determine the botanical origin of Malatya propolis for guiding beekeepers to place the beehives to the convenient areas.

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“…The challenge in developing propolis-based products lies in their standardization [2], a task that becomes intricate in countries with abundant biodiversity [18,19]. As a matter of fact, propolis from different regions of Chile have specific botanical origins of resins from Sorghum bicolor, Lotus sp., Acacia sp., Pinus vadiata, Eucalyptus sp., Salix babylonica, and Quillaja Saponaria [20].…”

Section: Introductionmentioning

confidence: 99%

Costa Rican Propolis Chemical Compositions: Nemorosone Found to Be Present in an Exclusive Geographical Zone

Umaña,

Solano,

Zamora

et al. 2023

Molecules

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Background: The chemistry of Costa Rican propolis from Apis mellifera remains underexplored despite its potential applications. This study identified its chemical composition, linking chemotypes to antioxidant potential. Methods: Proton nuclear magnetic resonance (1H NMR) spectra were obtained for 119 propolis extracts and analyzed using multivariate analyses. In parallel, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to assess antioxidant activity. A generalized linear regression model (GLM) correlated this with its chemical profiles and geographical origin. Chromatographic methods were used to isolate active and inactive compounds, which were identified using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS). Results: Principal component analysis (PCA) revealed three chemical profile groups for the 119 propolis extracts, explaining 73% of the total variance with two components. Radical scavenging activity was found to correlate with chemical composition. Isolation yielded n-coniferyl benzoate in type I (EC50 = 190 µg/mL, ORAC = 0.60 µmol TE/µmol) and nemorosone in type II (EC50 = 300 µg/mL, ORAC = 0.7 µmol TE/µmol). Type III was represented in terpene-like components, which exhibited lower antioxidant activity. Conclusions: This study categorizes Costa Rican propolis into three chemical types and identifies two key components linked to antioxidant activity. Notably, nemorosone, a valuable natural product, was found to be highly concentrated in a particular region of Costa Rica.

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An integrated strategy for chemical, biological and palynological standardization of bee propolis

Cited by 4 publications

References 35 publications

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

Palynological Analyses of Malatya Propolis Samples

Costa Rican Propolis Chemical Compositions: Nemorosone Found to Be Present in an Exclusive Geographical Zone

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