An innovative brilliant blue FCF method for fluorescent staining of fungi and bacteria

Chau, Henry Wai; Goh, YK; Cheng, Bing; Vujanovic, Vladimir

doi:10.3109/10520295.2010.492733

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…Using this method, tissue samples can be rapidly analyzed by histological analyses, such as immunohistochemistry and FISH, in order to screen genetic or protein markers, or to detect tissues infected with pathogenic/toxigenic factors. Since most dyes currently used for microbial fluorescent staining are toxic or carcinogenic, a tissue array system using brilliant blue FCF, which is a food dye and thus has no toxic effects, was developed and applied for microbial cell fluorescence staining of pathogenic/toxigenic and beneficial fungi and bacteria [ 107 ].…”

Section: Application Of Fluorescence-based Bioassaysmentioning

confidence: 99%

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Nishi

Isobe

Zhu

et al. 2015

Sensors

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We summarize here the recent progress in fluorescence-based bioassays for the detection and evaluation of food materials by focusing on fluorescent dyes used in bioassays and applications of these assays for food safety, quality and efficacy. Fluorescent dyes have been used in various bioassays, such as biosensing, cell assay, energy transfer-based assay, probing, protein/immunological assay and microarray/biochip assay. Among the arrays used in microarray/biochip assay, fluorescence-based microarrays/biochips, such as antibody/protein microarrays, bead/suspension arrays, capillary/sensor arrays, DNA microarrays/polymerase chain reaction (PCR)-based arrays, glycan/lectin arrays, immunoassay/enzyme-linked immunosorbent assay (ELISA)-based arrays, microfluidic chips and tissue arrays, have been developed and used for the assessment of allergy/poisoning/toxicity, contamination and efficacy/mechanism, and quality control/safety. DNA microarray assays have been used widely for food safety and quality as well as searches for active components. DNA microarray-based gene expression profiling may be useful for such purposes due to its advantages in the evaluation of pathway-based intracellular signaling in response to food materials.

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Section: Application Of Fluorescence-based Bioassaysmentioning

confidence: 99%

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Nishi

Isobe

Zhu

et al. 2015

Sensors

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“…enterocolitica, and Legionella pneumophila, with the lowest detection limit being 10 2 CFU/mL . In another study, Chau et al (2011) used RuBpy: [Ru(bpy)3]Cl2/Tris(bipyridine) ruthenium(II) chloride as a signal for sandwich fluorescent immunoassay to detect Staphylococcal enterotoxin B . Han et al (2013) employed an ELISA-based array using FluoSpheres to detect Staphylococcal enterotoxin B .…”

Section: Introductionmentioning

confidence: 99%

“… 26 In another study, Chau et al (2011) used RuBpy: [Ru(bpy)3]Cl2/Tris(bipyridine) ruthenium(II) chloride as a signal for sandwich fluorescent immunoassay to detect Staphylococcal enterotoxin B. 27 Han et al (2013) employed an ELISA-based array using FluoSpheres to detect Staphylococcal enterotoxin B. 28 These examples illustrate the versatility and widespread use of fluorescent labeling in microarray technology, which greatly aids in analyzing various biomolecular interactions.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of Multiplex Foodborne Pathogens

Hormsombut,

Mekjinda,

Kalasin

et al. 2024

ACS Appl. Bio Mater.

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Ensuring food safety is paramount for the food industry and global health concerns. In this study, we have developed a method for the detection of prevalent foodborne pathogenic bacteria, including Escherichia coli, Salmonella spp., Listeria spp., Shigella spp., Campylobacter spp., Clostridium spp., and Vibrio spp., utilizing antibody-aptamer arrays. To enhance the fluorescence signals on the microarray, the mesoporous silica nanoparticles (MSNs) conjugated with fluorescein, streptavidin, and seven detection antibodies-biotin were employed, forming fluorescein doped mesoporous silica nanoparticles conjugated with detection antibodies (MSNs-Flu-SA-Abs) complexes. The array pattern was designed for easy readability and enabled the simultaneous detection of all seven foodborne pathogens, referred to as the 7FP-biochip. Following the optimization of MSNs-Flu-SA-Abs complexes attachment and enhancement of the detection signal in fluorescent immunoassays, a high level of sensitivity was achieved. The detection limits for the seven pathogens in both buffer and food samples were 10 2 CFU/mL through visual screening, with fluorescent intensity quantification achieving levels as low as 20−34 CFU/g were achieved on the antibody-aptamer arrays. Our antibody-aptamer array offers several advantages, including significantly reduced nonspecific binding with no cross-reaction between bacteria. Importantly, our platform detection exhibited no cross-reactivity among the tested bacteria in this study. The multiplex detection of foodborne pathogens in canned tuna samples with spiked bacteria was successfully demonstrated in real food measurements. In conclusion, our study presents a promising method for detecting multiple foodborne pathogens simultaneously. With its high sensitivity and specificity, the developed antibody-aptamer array holds great potential for enhancing food safety and public health.

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“…Brilliant blue FCF is a water-soluble triphenylmethane dye, generally used as a water tracer agent . Due to its nontoxic activities, brilliant blue has been used as a food colorant as well as to stain cell bacterial and fungal cells. , Weber et al reported that brilliant blue shows an allergic reaction in some bronchial asthma patients . The photocytotoxicity of triphenylmethane dye depends on the production of reactive oxygen species with regard to their photodynamic therapy .…”

Section: Introductionmentioning

confidence: 99%

Photo-oxidative Decolorization of Brilliant Blue with AgNPs as an Activator in the Presence of K₂S₂O₈ and NaBH₄

et al. 2021

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The decolorization of brilliant blue (E133) in aqueous solution by K2S2O8 and NaBH4 with AgNPs as an activator was studied spectrophotometrically under normal laboratory conditions. Batch experiments were performed to investigate the effects of reaction time, initial dye concentration, activator concentration, solution pH, and temperature on the decolorization of E133. K2S2O8 and NaBH4 did not decolorize the dye E133 in the absence of AgNPs. The optimum dosage of AgNPs was 0.01 g/L, and 98% dye E133 degradation was observed with 3.75 mM K2S2O8 at 30 °C in ca. 60 min of reaction time. In the NaBH4/AgNPs system, only 60% dye degradation was observed for an identical reaction condition. The decolorization rate constant increases with the increase in concentrations of AgNPs, K2S2O8, NaBH4, and reaction temperature. The decolorization degree of the E133 responded linearly with K2S2O8 and NaBH4 concentrations. The existence of sulfate radicals (SO4 · –) and hydroxyl radicals (HO·) generated during the decolorization of E133 was identified by using ethanol and tertiary butyl alcohol as scavengers. Based on the E133 solution absorbance changes at 628 nm, the decolorization mechanism was proposed and discussed.

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An innovative brilliant blue FCF method for fluorescent staining of fungi and bacteria

Cited by 8 publications

References 23 publications

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of Multiplex Foodborne Pathogens

Photo-oxidative Decolorization of Brilliant Blue with AgNPs as an Activator in the Presence of K₂S₂O₈ and NaBH₄

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An innovative brilliant blue FCF method for fluorescent staining of fungi and bacteria

Cited by 8 publications

References 23 publications

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of Multiplex Foodborne Pathogens

Photo-oxidative Decolorization of Brilliant Blue with AgNPs as an Activator in the Presence of K2S2O8 and NaBH4

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Photo-oxidative Decolorization of Brilliant Blue with AgNPs as an Activator in the Presence of K₂S₂O₈ and NaBH₄