An Injectable Peptide Hydrogel Constructed of Natural Antimicrobial Peptide J-1 and ADP Shows Anti-Infection, Hemostasis, and Antiadhesion Efficacy

Abstract

Postoperative adhesion is a common complication of abdominal surgery, which always has many adverse effects in patients. At present, there is still a lack of effective treatment measures and materials to prevent adhesion in the clinics. Herein, we report the potential use of J-1-ADP hydrogel formed by natural antimicrobial peptide jelleine-1 (J-1) self-assembling in adenosine diphosphate (ADP) sodium solution to prevent postsurgery adhesion formation. J-1-ADP hydrogel was found to have good antimicrobial activity against the bacteria and fungi tested and can be used to prevent tissue infection, which was thought to be one of the incitements of adhesion. Due to ADP being a platelet-activating factor, J-1-ADP hydrogel showed significant hemostatic activity in vitro verified by whole blood coagulation, plasma coagulation, platelet activation, and platelet adhesion assays. Further, it showed potent hemostatic activity in a mouse liver hemorrhage model. Bleeding was believed to be a cause of the formation of postsurgery adhesion. J-1-ADP hydrogel had a significant antiadhesion effect in a rat side wall defect–cecum abrasion model. In addition, it had good biocompatibility and degradation properties. So the present study may provide an alternative strategy for designing antimicrobial peptide hydrogel material to prevent postoperative adhesion formation in the clinic.

Multifunctional Microgel-Based Cream Hydrogels for Postoperative Abdominal Adhesion Prevention

“…Moderate adhesions in the commercial Seprafilm group were formed, which is consistent with previous reports that show a substantial amount of tissue remaining on the wound site surface as a result of tight adhesions. 2,62 This might be because the membranes from Seprafilm were applied as a solid sheet and could not completely cover the traumatized surfaces. Additionally, the Seprafilm membrane might be randomly attached to the surface of injured tissues.…”

Bioinspired oral delivery devices

“…Bioinspired oral delivery devices will benefit from the incorporation of biomaterials made of biocompatible and functional peptides, molecules from traditional Chinese medicine and hydrogels 184 . The incorporation of such biomaterials can add more functions to oral delivery devices; for example, antimicrobial peptides isolated from the royal jelly of the honeybee can assemble into hydrogels and act as an anti-infective oral delivery device 184 . Furthermore, synthetic biology tools allow the engineering of microorganisms for oral applications; such microorganisms could produce bioactive molecules, including small molecules and proteins 72 .…”

Multifunctional 3D platforms for rapid hemostasis and wound healing: Structural and functional prospects at biointerfaces

“…The injectable hydrogel also displayed controlled platelet activation, adhesion, and blood coagulation owing to the presence of the J-1 peptide. Moreover, the fabricated hydrogel also showed substantial antibacterial properties against a broad range of bacteria (methicillin-resistant S. aureus [MRSA] and E. coli ) and fungi ( Candida albicans ) [ 135 ] . Therefore, antimicrobial hemostats can be used as a potential alternative to commercial hemostats and provide a favorable environment for platelet adhesion and new tissue formation with robust antimicrobial properties [ 136 ] .…”