2021
DOI: 10.3389/fpls.2021.706560
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Plant breeding relies on the meiotic recombination or crossing over to generate the new combinations of the alleles along and among the chromosomes. However, crossing over is constrained in the crops such as barley by a combination of the low frequency and biased distribution. In this study, we attempted to identify the genes that limit the recombination by performing a suppressor screen for the restoration of fertility to the semi-fertile barley mutant desynaptic10 (des10), carrying a mutation in the barley o… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
9
0

Year Published

2022
2022
2023
2023

Publication Types

Select...
5
2

Relationship

2
5

Authors

Journals

citations
Cited by 8 publications
(10 citation statements)
references
References 41 publications
1
9
0
Order By: Relevance
“…Combining results from all individuals, the M3 revealed an average of 20.16 ± 3.1 CO in Bowman and 10.64 ± 2.9 CO in BW230 M3 confirming the negative impact of mlh3/mlh3 on CO as shown previously ( Colas et al, 2016 ). Despite the numbers being relatively small (i.e., 175 chromosomes in each case) they are close to the mean cytological chiasma counts of 18.4 ± 1.3 for WT and 9.2 ± 2.1 for Hvmlh3 ( Colas et al, 2016 ) and the CO estimates derived from genetic mapping of 19.7 for WT and 7.1 for Hvmlh3 ( Arrieta et al, 2021a ) as well as being congruent with the mean CO numbers of 21.8 in WT populations estimated previously from genetic maps ( Close et al, 2009 ) suggesting few issues associated with comparing carefully conducted cytological analyses (despite the difficulties of resolution), and with traditional genetic analyses. While the number of CO is reduced in Hvmlh3 in comparison with WT the distribution along the chromosome does not seem to be affected ( Figure 6A ) which is again comparable to what has been observed by Colas et al (2016) and Arrieta et al (2021a) and to expectations given the role of MLH3 ( Colas et al, 2016 ).…”
Section: Discussionsupporting
confidence: 74%
See 1 more Smart Citation
“…Combining results from all individuals, the M3 revealed an average of 20.16 ± 3.1 CO in Bowman and 10.64 ± 2.9 CO in BW230 M3 confirming the negative impact of mlh3/mlh3 on CO as shown previously ( Colas et al, 2016 ). Despite the numbers being relatively small (i.e., 175 chromosomes in each case) they are close to the mean cytological chiasma counts of 18.4 ± 1.3 for WT and 9.2 ± 2.1 for Hvmlh3 ( Colas et al, 2016 ) and the CO estimates derived from genetic mapping of 19.7 for WT and 7.1 for Hvmlh3 ( Arrieta et al, 2021a ) as well as being congruent with the mean CO numbers of 21.8 in WT populations estimated previously from genetic maps ( Close et al, 2009 ) suggesting few issues associated with comparing carefully conducted cytological analyses (despite the difficulties of resolution), and with traditional genetic analyses. While the number of CO is reduced in Hvmlh3 in comparison with WT the distribution along the chromosome does not seem to be affected ( Figure 6A ) which is again comparable to what has been observed by Colas et al (2016) and Arrieta et al (2021a) and to expectations given the role of MLH3 ( Colas et al, 2016 ).…”
Section: Discussionsupporting
confidence: 74%
“…In an inbreeding crop plant such as barley, the routine approach to compare recombination rate and distribution in WT and meiotic mutants or disruptive transgenics has been to construct and compare genetic maps using high density genetic marker analysis ( Colas et al, 2016 , 2019 ; Arrieta et al, 2021a ). The approach generally uses the F3 progeny from multiple F2 individuals, that are homozygous for either WT or mutant alleles at the gene of interest, which are derived from a heterozygous F1 hybrid constructed from a cross between genetically diverse parents.…”
Section: Discussionmentioning
confidence: 99%
“…The molecular marker data revealed that COs increased in the majority of intervals tested, thus providing an opportunity to modulate recombination in wheat breeding programs. The distal H3K27me3-enriched regions experienced the greatest increase in COs, whereas the interstitial/proximal regions were more likely to be lower, similar to recombination increases in the barley recq4 mutant 49 .…”
Section: Discussionmentioning
confidence: 84%
“…On the other hand, the inactivation of the RECQ4 DNA helicase increased recombination by 3-4-fold with no concomitant fertility alteration in crops, including rice, pea, and tomato [49]. This positive result has been recently extended to a distant homeologous context in tomato [55] and to barley [56]. The inactivation of the DNA helicase RECQ4, therefore, appears to be a universal tool for stimulating meiotic recombination at the genome scale in crops.…”
Section: Modulation By Altering the Expression Of Genes Involved In H...mentioning
confidence: 94%