An in Vitro Evaluation of Antimicrobial Efficacy of 2% Chlorhexidine Gel, Propolis and Calcium Hydroxide Against Enterococcus faecalis in Human Root Dentin
Abstract:Aim: To evaluate in vitro the antimicrobial efficacy of 2% Chlorhexidine gel, Propolis and Calcium hydroxide against Enterococcus faecalis in human root dentin.
Methodology:One hundred and twenty human extracted anterior teeth were decoronated below CEJ and the apical part of root was removed to obtain 6mm of middle of the root. GG no 3 was used to standardize the internal diameter of root canal. Dentin blocks were infected with E faecalis for 21 d. They were assigned into four groups (n = 30).Group 1, Saline … Show more
“…The standard drugs used, fluconazole and calcium hydroxide, were selected according to the work described in the literature. These drugs are used for these microorganisms microflora endodontic (Warrilow et al, 2012;Maiolo et al, 2014;Bhandari et al, 2014).…”
This study evaluated the antimicrobial activity of the ethanol extract of Annona crassiflora Mart., against the fungus, Candida albicans present in the oral microbiota. The tests showed that the fractions of ethanol extract of the root bark and wood root of A. crassiflora showed a positive result. Of the strains studied, three showed sensitivity to 12 fractions and sub-fractions of A. crassiflora (66%). In the strains studied, strain 05 was the one that proved the most sensitive statistically (p <0.05). Their structures were determined using spectral techniques (NMR 1H and 13C) and based on literature data.
“…The standard drugs used, fluconazole and calcium hydroxide, were selected according to the work described in the literature. These drugs are used for these microorganisms microflora endodontic (Warrilow et al, 2012;Maiolo et al, 2014;Bhandari et al, 2014).…”
This study evaluated the antimicrobial activity of the ethanol extract of Annona crassiflora Mart., against the fungus, Candida albicans present in the oral microbiota. The tests showed that the fractions of ethanol extract of the root bark and wood root of A. crassiflora showed a positive result. Of the strains studied, three showed sensitivity to 12 fractions and sub-fractions of A. crassiflora (66%). In the strains studied, strain 05 was the one that proved the most sensitive statistically (p <0.05). Their structures were determined using spectral techniques (NMR 1H and 13C) and based on literature data.
“…Intracanal medicaments are regarded as an integral part of canal disinfection. Most commonly used medicament are calcium hydroxide and Chlorohexidine (1,2,3,5,7,14,15,16,17,18,19,20,21,22,23).…”
Section: Discussion:-mentioning
confidence: 99%
“…So, the search for other materials is done to improve the effect of intracanal medicaments against bacteria. (5,6,7) Ancient Egyptians and Greeks used natural materials in medicine (8). Among these natural materials used nowadays showing great expectations is propolis.…”
mentioning
confidence: 99%
“…Flavonoids give propolis its main antibacterial action. Among other properties of propolis are antiviral, antifungal, anti-inflammatory, and antioxidant (7,8,9,10). Propolis is found to be very useful in dentistry as an antibacterial agent in intracanal medications and irrigation solutions, also as a pulp capping agent, and in treatment of dentinal hypersensitivity (8,11).…”
The study aimed to evaluate the potential effect of three intracanal medicaments namely propolis, Calcium hydroxide and Chlorhexidine gel on the root canal disinfection. Materials and method: Sixty-Four samples were chosen for this study. They were divided into four groups, group I received no medication, group II received propolis, group III received Calcium Hydroxide and group IV received Chlorhexidine. The four groups were subdivided into two subgroups A and B, with two-time intervals 1 and 2 weeks. For bacterial evaluation the samples were inoculated with Enterococcus Faecalis. Paper points were placed inside infected root canals and placed in a test tube for CFU count. Results: Results showed that propolis had an intermediate effect on Enterococcus faecalis due to the presence of phenolic compounds in propolis which can eliminated bacteria inside root canal. Calcium hydroxide showed an intermediate effect against bacteria due to its high alkalinity that also can eliminate bacteria. Chlorhexidine showed the highest effect against bacteria due to its ability to stay active inside canal for a long time (substantivity). These results were similar for the 2 time periods. Conclusion: Both Propolis and Ca(OH)2 showed moderate action on intracanal microbes over the two time periods and CHX can be regarded as the best intracanal medicaments regarding its action on intracanal microbes.
“…Variadas substâncias são utilizadas no tratamento das infecções endodônticas, até mesmo os extratos naturais têm sido investigados como soluções irrigadoras, pois auxiliam no preparo químico-mecânico, na limpeza e na desinfecção do sistema de canais radiculares (9) . Nesse contexto, já se comprovou que a própolis exerce efeitos terapêuticos contra E. faecalis, podendo ser utilizada como uma alternativa eficaz no controle dessa bactéria (22) . Segundo os resultados da atual pesquisa, os extratos de Artemisia absinthium, Laurus nobilis e Bidens pilosa demonstraram serem eficientes para a inibição do crescimento bacteriano da S. mutans.…”
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