An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana

Antiuropathogenic and antioxidant activities of Hypoxis hemerocallidea Lam. extracts, and compounds from its taxonomically related species

“…The good antioxidant activity (LOD of 0.01 mg/mL) of galpinoside 5, and hemerocalloside 3, standard compounds whose antioxidant activity has never been reported alongside the methanol extract of the plant, is comparable to the positive control vitamin C. Whereas curculigoside C 2, has a moderate visual antioxidant activity at a concentration of 1 mg/mL and geraniol glycoside 1, had none. This results mirrors that reported by Mannathoko and co-workers [19] for similar antioxidant activity of 0.05 mg/mL for methanol extract of H. hemerocallidea corm and vitamin C (ascorbic acid). The mechanism of the anti-oxidant activity of galpinoside 5, and hemerocalloside 3, could be via the diacatechol-type (rooperol or nordihydroguaiaretic acid) pathway previously reported by other groups [20 -21].…”

“…This would imply that commercial products and muti that contain Hypoxis extracts and pure antioxidant compounds like galpinoside 5 and hemerocalloside 3 must maintain this optimal concentration for optimal anti-oxidant activity ceteris Paribus. The methanol extract, galpinoside 5 and hemerocalloside 3 has a quantitative free radical scavenging activity that is comparable to that of the positive control vitamin C. However, galpinoside 5 would be the compound with the best comparable antioxidant activity to vitamin C. The other analytes and vitamin C has linear absorbance after this concentration to indicate a possible similarity in their free radical scavenging potential compared to vitamin C to agree with results previously published [19][20]. The results obtained for the percentage free radical scavenging properties were 87.98% (IC 50 = 0.030 mg/mL) for galpinoside 5, compared with 89.93% (IC 50 = 0.028 mg/mL) of vitamin C and 87.20% (IC 50 = 0.037 mg/mL) for hemerocalloside 3, supports those previously reported [19,22] which highlighted that polar extracts of the fresh leaf and corms has high anti-oxidant activity associated with highly polar constituents.…”

Antioxidant and Antibacterial Activity of Methanol Extract of Momordica Balsamina

“…Coyle, 2005)and also reported elsewhere Mannathoko et al, 2017).In brief, 6mm diameter wells were made in the MHA agar plate with the base of a sterilized glass Pasteur pipette. Lawn cultures of each of the bacterial suspensions in Tryptone Soya broth (OXOID), adjusted to 0.5 McFarland standard turbidity, (equivalent to a bacterial suspension of 1.5 X 10 8 colony forming units per ml (CFU/ml)), were made on the MHA plates.…”

“…Unilab (South Africa). The silica gel coated aluminium backed TLC sheets were ready made Mannathoko et al, 2017). …”

“…Micro well dilution method with some modifications (Marie B. Coyle, 2005) was done to determine the minimal inhibitory concentration (MIC) of the extract for each of the microorganisms using 96 well plates Mannathoko et al, 2017).…”