An impedimetric biosensor for E. coli O157:H7 based on the use of self-assembled gold nanoparticles and protein G

Lin, Donghai; Pillai, Rahul; Lee, William; Jemere, Abebaw B.

doi:10.1007/s00604-019-3282-3

Cited by 68 publications

(27 citation statements)

References 42 publications

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“…3 Schematic illustration of (A) the structure and construction, and (B) detection and measurement process, of EIB nanoporous metal oxides and orderly structured carbon composites as the electrode substrate, to increase the surface area and sensitivity of EIBs (Ali et al, 2014;Ania et al, 2018;Bonanni et al, 2012;Chai and Takhistov, 2012). Metal nanoparticles have been used to intensify the electrochemical signal outputs from EIBs (Derkus et al, 2014;Lin et al, 2019;Peng et al, 2006).…”

Section: Structure and Construction Of Eibsmentioning

confidence: 99%

Electrochemical impedimetric biosensors for food safety

Chai

2020

Food Sci Biotechnol

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Electrochemical impedimetric biosensors (EIBs) have a simple structure and can be used to rapidly and sensitively detect and measure hazards in food. EIBs detect and measure target molecules by transducing biochemical reactions on their surface to electrical signal outputs responding to a sinusoidal electrical signal input. Due to their structural simplicity and analytical sensitivity, EIBs are regarded as the most potent method of food hazard monitoring that can be implemented in the food supply chain. This paper discusses the theoretical background, structure, and construction of EIB and its applications in food safety. Keywords Electrochemical impedance spectroscopy Á Biosensor Á Food safety Á Pathogen Á Mycotoxin max The maximum value of Z re R s The electrolyte resistance C dl The double-layer capacitance R ct The charge-transfer resistance e The dielectric constant d The thickness of the electrical double layer

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Section: Structure and Construction Of Eibsmentioning

confidence: 99%

Electrochemical impedimetric biosensors for food safety

Chai

2020

Food Sci Biotechnol

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“…Recently, Donghai Lin et al [ 69 ], have developed a simple and highly sensitive impedimetric immunosensor, based on the affinity reaction antibody-antigen establish on a paper electrode surface, for the sensitive detection of Escherichia coli O157:H7 bacteria. The integration of structural and electrical properties of reduced graphene oxide paper with a large active surface area of gold (Au) nanoparticles, for antibody immobilization, has shown a highly specific and stable detection of E. coli O157:H7, with a wide linear detection range 1.5 × 10 2 to 1.5 × 10 7 colony-forming unit.mL −1 , with a correlation coefficient of 0.9805.…”

Section: Electrochemical Methods Employed On Disease Diagnosismentioning

confidence: 99%

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

et al. 2020

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Over recent three decades, the electrochemical techniques have become widely used in biological identification and detection, because it presents optimum features for efficient and sensitive molecular detection of organic compounds, being able to trace quantities with a minimum of reagents and sample manipulation. Given these special features, electrochemical techniques are regularly exploited in disease diagnosis and monitoring. Specifically, amperometric electrochemical analysis has proven to be quite suitable for the detection of physiological biomarkers in monitoring health conditions, as well as toward the control of reactive oxygen species released in the course of oxidative burst during inflammatory events. Besides, electrochemical detection techniques involve a simple and swift assessment that provides a low detection-limit for most of the molecules enclosed biological fluids and related to non-transmittable morbidities.

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“…THz is a reagent-free, fast, and simple operational procedure, with great ability to differentiate between dead and living bacteria; however, the LOD of THz spectroscopy is still low compared to conventional culturing and molecular techniques . The best LOD reported was for E. coli (LOD = 10 4 CFU/mL) by Mazhorova et al (2012) while the LOD of an impedimetric biosensor for E. coli was reported as 48 CFU/mL (Lin, Pillai, Lee, & Jemere, 2019). A real-time PCR method showed the best LOD for detection of bacterial contaminates in food samples.…”

Section: Limited Sensitivity and Low Lod For Detection Of Microorganismsmentioning

confidence: 99%

“…The best LOD reported was for E. coli (LOD = 10 4 CFU/mL) by Mazhorova et al. () while the LOD of an impedimetric biosensor for E. coli was reported as 48 CFU/mL (Lin, Pillai, Lee, & Jemere, ). A real‐time PCR method showed the best LOD for detection of bacterial contaminates in food samples.…”

Section: Current Pitfalls and Future Prospects Of Thz Technologymentioning

confidence: 99%

A Comprehensive Review on Food Applications of Terahertz Spectroscopy and Imaging

Afsah‐Hejri

Hajeb

Ara

et al. 2019

Comp Rev Food Sci Food Safe

209

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Food product safety is a public health concern. Most of the food safety analytical and detection methods are expensive, labor intensive, and time consuming. A safe, rapid, reliable, and nondestructive detection method is needed to assure consumers that food products are safe to consume. Terahertz (THz) radiation, which has properties of both microwave and infrared, can penetrate and interact with many commonly used materials. Owing to the technological developments in sources and detectors, THz spectroscopic imaging has transitioned from a laboratory‐scale technique into a versatile imaging tool with many practical applications. In recent years, THz imaging has been shown to have great potential as an emerging nondestructive tool for food inspection. THz spectroscopy provides qualitative and quantitative information about food samples. The main applications of THz in food industries include detection of moisture, foreign bodies, inspection, and quality control. Other applications of THz technology in the food industry include detection of harmful compounds, antibiotics, and microorganisms. THz spectroscopy is a great tool for characterization of carbohydrates, amino acids, fatty acids, and vitamins. Despite its potential applications, THz technology has some limitations, such as limited penetration, scattering effect, limited sensitivity, and low limit of detection. THz technology is still expensive, and there is no available THz database library for food compounds. The scanning speed needs to be improved in the future generations of THz systems. Although many technological aspects need to be improved, THz technology has already been established in the food industry as a powerful tool with great detection and quantification ability. This paper reviews various applications of THz spectroscopy and imaging in the food industry.

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An impedimetric biosensor for E. coli O157:H7 based on the use of self-assembled gold nanoparticles and protein G

Cited by 68 publications

References 42 publications

Electrochemical impedimetric biosensors for food safety

Electrochemical impedimetric biosensors for food safety

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

A Comprehensive Review on Food Applications of Terahertz Spectroscopy and Imaging

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