An impedance biosensor for monitoring cancer cell attachment, spreading and drug-induced apoptosis

Nguyen, Trung-Thanh; Tiberius, Bogdan; Pliquett, Uwe; Urban, G.

doi:10.1016/j.sna.2016.02.035

Cited by 55 publications

(47 citation statements)

References 22 publications

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“…Table 1 summarizes some examples of anticancer drugs that have been evaluated by impedance-based methods. Single cell or few cell populations [16], large cell populations, and three-dimensional (3D) cell aggregates, such as cell spheroids [17,18], have been tested. A recent report by Seidel et al [19] focused on the evaluation of derived two-dimensional (2D) and 3D cell cultures with original patient undissociated melanoma tissues in order to develop cell model data of combined targeted cancer therapy and to transfer these data to an in vivo situation [19].…”

Section: Impedance-based Methods For Cell Monitoringmentioning

confidence: 99%

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Tadini‐Buoninsegni

Palchetti

2020

Sensors

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Cancer is a multifactorial family of diseases that is still a leading cause of death worldwide. More than 100 different types of cancer affecting over 60 human organs are known. Chemotherapy plays a central role for treating cancer. The development of new anticancer drugs or new uses for existing drugs is an exciting and increasing research area. This is particularly important since drug resistance and side effects can limit the efficacy of the chemotherapy. Thus, there is a need for multiplexed, cost-effective, rapid, and novel screening methods that can help to elucidate the mechanism of the action of anticancer drugs and the identification of novel drug candidates. This review focuses on different label-free bioelectrochemical approaches, in particular, impedance-based methods, the solid supported membranes technique, and the DNA-based electrochemical sensor, that can be used to evaluate the effects of anticancer drugs on nucleic acids, membrane transporters, and living cells. Some relevant examples of anticancer drug interactions are presented which demonstrate the usefulness of such methods for the characterization of the mechanism of action of anticancer drugs that are targeted against various biomolecules.

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Section: Impedance-based Methods For Cell Monitoringmentioning

confidence: 99%

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Tadini‐Buoninsegni

Palchetti

2020

Sensors

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“…ECIS provides the average dielectric signature for a cell population, which is important for continuous real-time monitoring of cells. Adhered cells going through apoptosis have lower impedance values due to the cell membrane being compromised, and it has been found that ECIS can sense when anti-cancer drugs induce apoptosis in cancer cells [ 14 , 43 ]. Thus, impedance can be used to assess cell death and protein secretion during apoptosis.…”

Section: Electrical Impedance Spectroscopy (Eis)mentioning

confidence: 99%

“…Apoptosis, also known as “cell suicide”, is the process in which cells are programmed to be removed in order to maintain the overall health of the organism. Necrosis differs because cells die due to injury or other external factors [ 14 ]. Apoptosis and necrosis are important in understanding chemoresistance and the removal of dead cells from these processes will impact impedance.…”

Section: Applications Of Impedance Spectroscopy For Cancer Cellsmentioning

confidence: 99%

“…EIS relies on cell polarization generated by an electric field and the interaction of ions along the cell surface [ 13 ]. Impedance-based biosensors have been used to distinguish many types of human and mouse cancer cells based on their dielectric properties (impedance and capacitance) [ 14 , 15 , 16 , 17 ]. EIS is advantageous because it enables real time measurements of cell dynamics.…”

Section: Introductionmentioning

confidence: 99%

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Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

et al. 2020

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Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

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“…Two microchannels of 40 μm in height were formed by a natural adhesion of the PDMS cover onto the microchannel walls. The design and fabrication of a proposed sensor chip can be found in details in our previous works [11,13].…”

Section: A Microfluidic Chip Design and Fabrication Processmentioning

confidence: 99%

Microfluidic Impedance Biosensors for Monitoring a Single and Multiple Cancer Cells in Anticancer Drug Treatments

Nguyen

Tran

et al. 2017

6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6)

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An impedance biosensor for monitoring cancer cell attachment, spreading and drug-induced apoptosis

Cited by 55 publications

References 22 publications

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Microfluidic Impedance Biosensors for Monitoring a Single and Multiple Cancer Cells in Anticancer Drug Treatments

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