An Immunopharmacological Study of the Biphasic Allergic Skin Reaction in Mice.

Nagai, Hiroichi; Sakurai, Tsuyoshi; Inagaki, Nobuya; Mori, Hiroshi

doi:10.1248/bpb.18.239

Cited by 45 publications

(68 citation statements)

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“…In this model mice are sensitized with IgE and subsequently challenged with DNFB (42). This challenge results in a biphasic inflammatory response, an early phase that peaks at 1 h, and a late phase that peaks at 24 h (42).…”

Section: Discussionmentioning

confidence: 99%

Role of STAT6 in IgE Receptor/FcεRI-Mediated Late Phase Allergic Responses of Mast Cells

Malaviya

Uckun

2002

The Journal of Immunology

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In this study we show that IgE receptor engagement triggers activation of STAT6 in mast cells. We sought to determine the role of STAT6 activation in IgE receptor-mediated mast cell responses using STAT6 knockout mice. After IgE receptor engagement, bone marrow mast cells from STAT6−/− mice exhibited normal histamine and leukotriene C4 release, but their cytokine release was markedly reduced. In accordance with these in vitro data, IgE/Ag-challenged STAT6−/− mice showed normal early phase, but severely impaired late phase, allergic reactions. These findings provide unprecedented evidence that STAT6 plays a pivotal role in mast cell responses to IgE/Ag st imulation.

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Section: Discussionmentioning

confidence: 99%

Role of STAT6 in IgE Receptor/FcεRI-Mediated Late Phase Allergic Responses of Mast Cells

Malaviya

Uckun

2002

The Journal of Immunology

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“…In addition, as for high dose of DSCG, the effect on LPR was weaker than that on EPR. It was reported that DSCG inhibited both EPR and LPR, but most mast cell stabilizers inhibited only EPR and showed no effect on LPR (2,21,22). The concentration of DSCG required to suppress cytokine production from T cells was 100-to 1000-fold higher than that required for mast cell stabilization (23).…”

Section: Discussionmentioning

confidence: 99%

A Late Cutaneous Response in Actively Sensitized Rats: a New Method for Evaluating the Efficacy of Antiallergic Drugs

et al. 2006

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Abstract. We established a new and facile model to investigate allergic mechanism and assess the effect of antiallergic compounds. Male Wistar rats were actively or passively sensitized. Active sensitization was performed by injection of both dinitrophenylated-ovalbumin (DNP-OA) and Bordetella pertussis. Nine days later, DNP-OA was injected into the right hind footpad. This antigen challenge induced a biphasic footpad swelling that consisted of an early-phase (EPR) and a late-phase response (LPR). In rats passively sensitized with rat anti-DNP-OA serum, DNP-OA induced only EPR. The EPR was suppressed by disodium cromoglycate, a mast cell stabilizer, but not by cyclosporin A, an immunosuppressant, while the LPR was suppressed by cyclosporin A. Furthermore, to investigate these two allergic responses determined by the interactions between the hapten and the carrier proteins, two distinct haptenated antigens were created. DNP-Ascaris (DNP-As) induced a marked EPR and LPR in DNP-As-sensitized rats. However, DNP-As induced only EPR in DNP-OA-sensitized rats, indicating that the usage of the same carrier protein in both sensitization and challenge was necessary for induction of LPR. These data suggest that this actively sensitization model in which EPR and LPR are functionally distinguishable should be useful for evaluating the efficacy of antiallergic compounds.

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“…The first phase ear swelling is dependent on mast cells and inhibited by mast cell stabilizers and antihistamines (14). In contrast, inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β seem to participate in the second phase response (15,16).…”

Section: Triphasic Cutaneous Reaction In the Mouse Earmentioning

confidence: 99%

Analysis of the Mechanism for the Development of Allergic Skin Inflammation and the Application for Its Treatment: Mouse Models for the Development of Remedies for Human Allergic Dermatitis

Inagaki

Nagai

2009

J Pharmacol Sci

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Abstract. Animal models for human diseases are important for the elucidation of mechanisms involved in as well as for the establishment of effective treatment strategies for the diseases. Many mouse allergic dermatitis models have been established and applied for the development of remedies for human allergic dermatitis. One of the simplest allergic cutaneous reaction models is passive cutaneous anaphylaxis. Skin mast cells passively sensitized with antigen-specific IgE are activated upon antigen challenge to cause vascular permeability increase. The triphasic cutaneous reaction is another interesting model in which triphasic cutaneous swelling appears in mice passively sensitized with IgE upon challenge with specific antigen. Recently, many allergic dermatitis models have been established by repeated antigen exposure. Although the induced dermatitis seems to be complex, these models may possess the characteristic features observed in patients. Naturally occurring dermatitis model mice such as NC/Nga, NOA, and DS-Nh mice, and gene-knockout and transgenic mice exhibiting spontaneous dermatitis are also known. Establishment and characterization of the mouse dermatitis models seem to be important for understanding dermatitis and development of new strategies for the treatment of these diseases.

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An Immunopharmacological Study of the Biphasic Allergic Skin Reaction in Mice.

Cited by 45 publications

References 0 publications

Role of STAT6 in IgE Receptor/FcεRI-Mediated Late Phase Allergic Responses of Mast Cells

Role of STAT6 in IgE Receptor/FcεRI-Mediated Late Phase Allergic Responses of Mast Cells

A Late Cutaneous Response in Actively Sensitized Rats: a New Method for Evaluating the Efficacy of Antiallergic Drugs

Analysis of the Mechanism for the Development of Allergic Skin Inflammation and the Application for Its Treatment: Mouse Models for the Development of Remedies for Human Allergic Dermatitis

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