An immunoelectron microscopic localization of noncollagenous bone proteins (osteocalcin and osteopontin) at the bone–titanium interface of rat tibiae

Ayukawa, Yasunori; Takeshita, Fumitaka; Inoue, Takashi; Yoshinari, Masao; Shimono, Masaki; Suetsugu, Tsuneo; Takano, Teruo

doi:10.1002/(sici)1097-4636(199807)41:1<111::aid-jbm14>3.3.co;2-r

Cited by 14 publications

(15 citation statements)

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“…Osteopontin has been shown to inhibit the crystal growth of hydroxyapatite e.g. [22], while osteonectin has been suggested to promote collagen mineralization [23]. In the present study, no difference in osteocalcin, osteopontin and osteonectin gene expression was found at week 1 between the samples.…”

Section: Discussioncontrasting

confidence: 55%

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Ku¹,

et al. 2002

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The purpose of the present work was to examine the effect of different Ti-6Al-4V surface treatments on osteoblasts behaviour. Previous work in this laboratory has demonstrated that an ageing treatment reduces metal ion release from this alloy compared to standard passivation procedures. In this study, human osteosarcoma MG-63 were used in short-term in vitro tests to assay for cell viability and cell proliferation at 12, 24 and 72 h while SaOS-2 were used in long-term in vitro tests to assay for osteonectin, osteopontin, osteocalcin gene expression, total protein amount (TP), alkaline phosphatase activity (ALP) and fibronectin production (FN) for 1-4 weeks. Epifluorescence microscopy was used to observe SaOS-2 cell morphology. After 24 h, there was no difference in MG-63 cell viability/proliferation or in SaOS-2 cell morphology between the different surface treatments. For the longterm tests, the aged Ti-6Al-4V induced significantly higher cell proliferation than the control Ti-6Al-4V at 72 h. At week 1, no difference in the osteonectin, osteopontin, and osteocalcin gene expression was found between samples. The peak of ALP activity appeared earlier at week 2 for the control surface compared with the passivated and aged surfaces. The early increase in ALP activity for the control sample could be a compensatory effect of decreased osteoblasts proliferation. There was no difference in the expression of FN for the different surface treatments. Our present results showed that the different surface treatments, which induced different metal ion release kinetics and surface properties, influenced the cell proliferation and ALP activity of osteoblast cells. Aluminium ions release kinetics as well as presence of vanadium ions may play a major role in influencing the osteoblasts behaviour in the present study. r

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Section: Discussioncontrasting

confidence: 55%

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Ku¹,

et al. 2002

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“…ions, which then react with Ca-binding proteins or glycosaminoglycans, such as osteocalcin, osteopontin or heparan sulfate proteoglycan (HSPG) [24]. Indeed, some reports suggest that osteocalcin and osteopontin are key factors in achieving osteoblastic cell adhesion to dental implants [25,26].…”

Section: Discussionmentioning

confidence: 99%

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl2 (Ca) or MgCl2 (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

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“…7) Recently, in an ultrastructural study, Ayukawa et al found a thin amorphous zone at the osseointegrated layer between the titanium and bone interface. [8][9][10] Based on this investigation, it can be speculated on the process occurring in the implant hosts (Fig. 4).…”

Section: Osseointegration Of Titaniummentioning

confidence: 99%

Bio-Functionalization of Titanium Surfaces for Dental Implants

Yoshinari¹,

Matsuzaka

Inoue

et al. 2002

Mater. Trans.

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Since dental implants are used in contact with many different tissues, it is necessary to have optimum surface compatibility with the host bone tissues and soft tissues. Furthermore, dental implant surfaces exposed to the oral cavity must remain plaque-free. Such materials can be created under well-controlled conditions by modifying the surfaces of metals that contact those tissues. "Tissue-compatible implants," which are compatible with all host tissues, must integrate with bone tissue, easily form hemidesmosomes, and prevent bacterial adhesion. This research was aimed at developing such tissue-compatible implants by modifying titanium surfaces using a dry process for closely adhering to the titanium substrate and ensuring good wear resistance. The process includes ion beam dynamic mixing (thin calcium phosphates), ion implantation, titania spraying, ion plating and ion beam mixing. At the bone tissue/implant interface, a thin calcium phosphate coating and rapid heating with infrared radiation was effective in controlling the dissolution without cracking the coating. This thin calcium phosphate coating may directly promote osteogenisis, but also enable immobilization of functional proteins or drugs such as bisphosphonate for dug delivery system. At the oral fluid/implant interface, an alumina coating and F + -implantation were responsible for inhibiting the adhesion of microbial plaque. In conclusion, dry-process surface modification is useful in controlling the physicochemical nature of surfaces, including the surface energy and the surface electrical charge, and in developing tissue-compatible implants.

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An immunoelectron microscopic localization of noncollagenous bone proteins (osteocalcin and osteopontin) at the bone–titanium interface of rat tibiae

Cited by 14 publications

References 0 publications

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Bio-Functionalization of Titanium Surfaces for Dental Implants

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