An fgf21-like gene from swamp eel (Monopterus albus): Recombinant expression and its potential roles in glucose and lipid homeostasis

FGF21 Improves Glycolipid Metabolism in Rainbow Trout (Oncorhynchus mykiss) Fed a High-Carbohydrate Diet by Inhibiting Inflammatory Responses and Activating Autophagy

“…At the mRNA level, the downregulation of the expression of gluconeogenesis-related genes pepck and g6pase and concurrent upregulation in the expression of gk and glut2 implied that FGF21 plays a regulatory role in glucose metabolism. These results were corroborated in other species. ,, Although the changes in these genes were slightly different on days 3, 7, and 10 after a single injection, this means that the effect of exogenous injection of rFGF21 could be maintained for about a week or so, which could provide a partial reference for subsequent experiments. The mechanism underlying hypoglycemia in animals may be associated with hepatic glycogen metabolism.…”

“…These results were corroborated in other species. 19,22,27 Although the changes in these genes were slightly different on days 3, 7, and 10 after a single injection, this means that the effect of exogenous injection of rFGF21 could be maintained for about a week or so, which could provide a partial reference for subsequent experiments. The mechanism underlying hypoglycemia in animals may be associated with hepatic glycogen metabolism.…”

“…19,20 Furthermore, exogenous recombinant FGF21 suppressed appetite in Asian sea bass, 21 and fgf 21 overexpression in swamp eel protected against glucose stress. 22 Rainbow trout is a valuable fish that is widely farmed, but the mechanisms regulating glycolipid metabolism have not been thoroughly investigated as a representative of "glucose intolerance" fish. Therefore, this study aimed (1) to clone the rainbow trout fgf 21, prepare the recombinant protein, and evaluate its protective effect on rainbow trout under glucose stress; (2) to evaluate the regulation of metabolism in rainbow trout by rFGF21 under its profile of high-carbohydrate diets; and (3) to investigate the potential mechanisms through which rFGF21 modulates metabolic activities.…”

“…Specifically, only four studies were undertaken on fish FGF21. The expression of fgf21 in grass carp and zebrafish has been found to be correlated with food intake. , Furthermore, exogenous recombinant FGF21 suppressed appetite in Asian sea bass, and fgf21 overexpression in swamp eel protected against glucose stress . Rainbow trout is a valuable fish that is widely farmed, but the mechanisms regulating glycolipid metabolism have not been thoroughly investigated as a representative of “glucose intolerance” fish.…”

Analysis of Functional Promoter of Camel FGF21 Gene and Identification of Small Compounds Targeting FGF21 Protein

“…FGF21 is a hormone that regulates glucose–lipid metabolic pathways, including stimulating the oxidation of fatty acids, production of ketone bodies, inhibition of lipogenesis, glucose uptake, amino acid transport, and energy expenditure [ 4 , 5 , 6 ]. Thus, FGF21 is involved in many metabolic diseases, such as obesity, diabetes, and non-alcoholic fatty liver [ 7 , 8 , 9 ], making it a promising therapeutic target for these metabolic diseases. Actually, FGF21 analogs LY2405319, PF-05231023, etc., were proven to be effective therapeutic agents in obesity, type 2 diabetes mellitus (T2DM), non-alcohol fatty liver disease (NAFLD) and cholestatic liver disease [ 10 , 11 ].…”