An experimental model for Staphylococcus aureus hepatic abscess in Bama minipig

Abstract

ABSTRACT. Pyogenic hepatic abscess (PHA) is a rare but potentially serious disease. Investigations of new therapeutic methods urgently need experimental support in corresponding animal models. However, to date, few studies have evaluated PHA in the minipig. The linear regression equation of the Staphylococcus aureus ATCC 25923 strain was established. PHA was successfully mocked, and S. aureus ATCC 29213 was the only pathogenic bacterium identified. The abscess formation stage was observed on the 21st day of the operation. This study will provide a baseline for further studies evaluating new treatment methods for PHA.

Alcoholization of pyogenichepatic abscess with absolute alcohol in Bama minipigs

“…Autologous venous blood was acquired from the splenic vein when the animal abdominal cavity was exposed and autologous venous blood clot was acquired when the blood was automatically coagulated, as previously described (29). After day 21, the animals were diagnosed with PHA and were at abscess-formation stage (29). Next, they were randomly allocated into two groups (15), including the alcoholization treatment and the physiological saline (PS) control groups.…”

“…PS was used in the other group, which acted as a negative control. All animals were sacrificed by exsanguination through the femoral artery under full sedation using intramuscular injection of a solution containing a mixture of zolazepam, tiletamine, xylazine and ketamine (0.83 mg/kg BW of each drug) and butorphanol (0.17 mg/kg BW) (29). After 21 days of the alcoholization, autopsy was performed for assessing the therapeutic efficacy of the treatment.…”

“…Subsequent to exposing the abdominal cavity of the animals, a mixture of methicillin-sensitive S. aureus (ATCC 29213; American Type Culture Collection, Manassas, VA, USA) and autologous venous blood clot was quickly injected into the liver parenchyma. Autologous venous blood was acquired from the splenic vein when the animal abdominal cavity was exposed and autologous venous blood clot was acquired when the blood was automatically coagulated, as previously described (29). After day 21, the animals were diagnosed with PHA and were at abscess-formation stage (29).…”

“…The body weight and rectal temperature were respectively measured with a health meter and mercury thermometer. Venous blood was obtained from the vena cava anterior in order to examine the white blood cell (WBC) count; a catheter (22G) was inserted into the right ear vein to infuse an anesthetic (29).…”

“…The present study established a PHA model in Bama minipigs (29), and the animals were then prospectively treated with the alcoholization. The results demonstrated that the alcoholization is a safe, effective and well-tolerated method to manage PHA.…”