An Experimental and Modeling Study on the Pretreatment and Alkaline Hydrolysis of Blue Agave Bagasse in Twin-Screw Extruders

Morales‐Huerta, Juan Carlos; Hernández-Meléndez, Óscar; Hernández-Luna, Martín; Manero, Ο.; Bárzana, Eduardo; Vivaldo‐Lima, Eduardo

doi:10.1021/acs.iecr.1c02175

Cited by 10 publications

(25 citation statements)

References 58 publications

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“…There have hitherto been developed various pretreatment strategies regarding the productivity, yield, and number of steps to enhance the hydrolysis of lignocellulose materials. Over the past few years, pilot-scale operational conditions have been proposed for alkaline [ 34 , 35 ], hydrothermal [ 36 ], and ultra-fast hydrolysis by supercritical CO 2 pretreatments. More recently, Ferri et al [ 6 ] demonstrated a robust and eco-friendly three-step biorefinery process for the efficient release of FA.…”

Section: Resultsmentioning

confidence: 99%

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Radenkovs

Juhnevica-Radenkova

Kviesis

et al. 2021

Foods

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Biorefining by enzymatic hydrolysis (EH) of lignocellulosic waste material due to low costs and affordability has received enormous interest amongst scientists as a potential strategy suitable for the production of bioactive ingredients and chemicals. In this study, a sustainable and eco-friendly approach to extracting bound ferulic acid (FA) was demonstrated using single-step EH by a mixture of lignocellulose-degrading enzymes. For comparative purposes of the efficiency of EH, an online extraction and analysis technique using supercritical fluid extraction–supercritical fluid chromatography–mass spectrometry (SFE-SFC-MS) was performed. The experimental results demonstrated up to 369.3 mg 100 g−1 FA release from rye bran after 48 h EH with Viscozyme L. The EH of wheat and oat bran with Viscoferm for 48 h resulted in 255.1 and 33.5 mg 100 g−1 of FA, respectively. The release of FA from bran matrix using supercritical fluid extraction with carbon dioxide and ethanol as a co-solvent (SFE-CO2-EtOH) delivered up to 464.3 mg 100 g−1 of FA, though the extractability varied depending on the parameters used. The 10-fold and 30-fold scale-up experiments confirmed the applicability of EH as a bioprocessing method valid for the industrial scale. The highest yield of FA in both scale-up experiments was obtained from rye bran after 48 h of EH with Viscozyme L. In purified extracts, the absence of xylose, arabinose, and glucose as the final degradation products of lignocellulose was proven by high-performance liquid chromatography with refractive index detection (HPLC-RID). Up to 94.0% purity of FA was achieved by solid-phase extraction (SPE) using the polymeric reversed-phase Strata X column and 50% EtOH as the eluent.

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Section: Resultsmentioning

confidence: 99%

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Radenkovs

Juhnevica-Radenkova

Kviesis

et al. 2021

Foods

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show abstract

“…There have hitherto been developed various pretreatment strategies regarding the productivity, yield, and the number of steps to enhance the hydrolysis of lignocellulose materials. Over the last years, pilot-scale operational conditions have been proposed for alkaline [37,38], hydrothermal [39], and ultra-fast hydrolysis by supercritical CO2 pretreatments. More recently, Ferri et al [6] demonstrated a robust and eco-friendly three-step biorefinery solution for the efficient release of FA.…”

Section: Release Of Fa From Bran Using Enzyme-assisted Hydrolysis Of 10-and 30-fold Scale-up Processmentioning

confidence: 99%

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Radenkovs¹,

Juhnevica-Radenkova²,

Kviesis³

et al. 2021

Preprint

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Biorefining by enzymatic hydrolysis (EH) of lignocellulosic waste material due to low costs and affordability has received enormous interest amongst scientists as a potential strategy suitable for the production of bioactive ingredients and chemicals. In the present study, a sustainable and eco-friendly approach to the extraction of bound ferulic acid (FA) has been demonstrated using a single-step EH by a mixture of lignocellulose-degrading enzymes. For comparative purposes of the efficiency of EH, an online SFE-SFC-MS extraction and analysis approach was applied. The experimental results demonstrated up to 369.3 mg 100 g−1 FA released from rye bran after 48 h EH with Viscozyme L. The EH of wheat and oat bran with Viscoferm for 48 h resulted in 255.1 and 33.5 mg 100 g−1 of FA, respectively. The extraction of FA from bran matrix using the SFE-CO2-EtOH delivered up to 464.3 mg 100 g−1 of FA, though the extractability varied depending on the parameters used. The 10-fold and 30-fold scale-up experiments confirmed the applicability of EH as a bioprocessing method valid for industrial-scale. The highest yield of FA in both scale-up experiments was obtained from rye bran after 48 h of EH with Viscozyme L. In purified extracts, the absence of xylose, arabinose, and glucose as final degradation products of lignocellulose was proven by a HPLC-RID system. Up to 94.0% purity of FA was achieved by SPE using the polymeric reversed-phase Strata X column and 50% EtOH as eluent.

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“…Others have reported alkaline decomposition kinetics of lignin model compounds (Gierer et al, 1977;Shimizu et al, 2012;Shimizu et al, 2013), although these insights are not readily extensible to real biomass, where cell wall biopolymer assemblies and tissue pore structures at the nanoscale and mesoscale, respectively, heavily influence experimental kinetic measurements (Min et al, 2014;Lima et al, 2018). Indeed, given the complexity surrounding experimental diffusion measurements (Jakes et al, 2020), few reports attempt to quantify the significant mass or heat transfer effects that accompany deacetylation of real biomass (Costanza and Costanza, 2002), especially as they apply to complicated continuous reactor configurations such as packed corn stover beds (Sahayaraj et al, 2021) or twin-screw extruders (Morales-Huerta et al, 2021). Lastly, the vast majority of these studies employ whole (unsorted) corn stover as the feedstock, despite the known structural and compositional differences among its major anatomical fractions (Min et al, 2014), which are expected to imbue unique kinetic and physical transport properties.…”

Section: Introductionmentioning

confidence: 99%

Mass Transport Limitations and Kinetic Consequences of Corn Stover Deacetylation

et al. 2022

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Alkaline pretreatment of herbaceous feedstocks such as corn stover prior to mechanical refining and enzymatic saccharification improves downstream sugar yields by removing acetyl moieties from hemicellulose. However, the relationship between transport phenomena and deacetylation kinetics is virtually unknown for such feedstocks and this pretreatment process. Here, we report the development of an experimentally validated reaction–diffusion model for the deacetylation of corn stover. A tissue-specific transport model is used to estimate transport-independent kinetic rate constants for the reactive extraction of acetate, hemicellulose and lignin from corn stover under representative alkaline conditions (5–7 g L−1 NaOH, 10 wt% solids loadings) and at low to mild temperatures (4–70°C) selected to attenuate individual component extraction rates under differential kinetic regimes. The underlying transport model is based on microstructural characteristics of corn stover derived from statistically meaningful geometric particle and pore measurements. These physical descriptors are incorporated into distinct particle models of the three major anatomical fractions (cobs, husks and stalks) alongside an unsorted, aggregate corn stover particle, capturing average Feret lengths of 917–1239 μm and length-to-width aspect ratios of 1.8–2.9 for this highly heterogeneous feedstock. Individual reaction–diffusion models and their resulting particle model ensembles are used to validate and predict anatomically-specific and bulk feedstock performance under kinetic-controlled vs. diffusion-controlled regimes. In general, deacetylation kinetics and mass transfer processes are predicted to compete on similar time and length scales, emphasizing the significance of intraparticle transport phenomena. Critically, we predict that typical corn stover particles as small as ∼2.3 mm in length are entirely diffusion-limited for acetate extraction, with experimental effectiveness factors calculated to be 0.50 for such processes. Debilitatingly low effectiveness factors of 0.021–0.054 are uncovered for cobs—implying that intraparticle mass transfer resistances may impair observable kinetic measurements of this anatomical fraction by up to 98%. These first-reported quantitative maps of reaction vs. diffusion control link fundamental insights into corn stover anatomy, biopolymer composition, practical size reduction thresholds and their kinetic consequences. These results offer a guidepost for industrial deacetylation reactor design, scale-up and feedstock selection, further establishing deacetylation as a viable biorefinery pretreatment for the conversion of lignocellulosics into value-added fuels and chemicals.

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An Experimental and Modeling Study on the Pretreatment and Alkaline Hydrolysis of Blue Agave Bagasse in Twin-Screw Extruders

Cited by 10 publications

References 58 publications

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Mass Transport Limitations and Kinetic Consequences of Corn Stover Deacetylation

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