An exopolysaccharide from a probiotic: Biosynthesis dynamics, composition and emulsifying activity

Kodali, Vidya Prabhakar; Das, Shrilekha; Sen, Ramkrishna

doi:10.1016/j.foodres.2009.02.007

Cited by 84 publications

(48 citation statements)

References 22 publications

(25 reference statements)

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“…These are the most important and desirable properties of an organism to qualify as a potential probiotic. In addition to these, two of our recently published articles convincingly demonstrated that an exopolysaccharide (EPS) produced by our strain Bacillus coagulans RK -02 possessed very significant probioactive properties like antioxidant [28] and emulsification [29], and antihyperglycemic activities [30]. Like fructooligosaccharide prebiotics, EPS produced in situ by our RK -02 strain may be effective in playing a positive role in the colonization of GIT [22,27].…”

Section: Discussionmentioning

confidence: 72%

Molecular Characterization and In Vitro Analyses of a Sporogenous Bacterium with Potential Probiotic Properties

Sen

Pal

Kodali

et al. 2010

Probiotics & Antimicro. Prot.

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The Gram-positive thin rods of a Bacillus species were identified and designated as Bacillus coagulans RK - 02 through the standard microbiological and biochemical characterization procedures, followed by data analysis and comparison with the characteristics given in Bergey's manual of systematic bacteriology. The culture was further characterized and confirmed as Bacillus coagulans by 16S rDNA sequence analysis wherein about 755 nucleotides of 16S rDNA was amplified and cloned in pGEM-T vector and subsequently sequenced. Sequence was blasted against the nr database of NCBI. Multiple alignments were done with some selected and related sequences using Clustal W. Phylogenetic trees were drawn with the same software after the distances were determined by neighbor-joining algorithm. The in vitro studies on the probiotic properties demonstrated that our isolate could prove to be a potential probiotic with spore-forming and lactic acid-producing abilities coupled with acid and bile tolerance properties and antimicrobial action. In addition to these characteristics, the bacterium also produced enzymes such as amylase, cellulase, lipase, protease, lactase and catalase, which can help in improving digestion and overall health, alleviate lactose intolerance and remove oxidative stresses, required for the well-being of the consumers. In our previously reported studies, an exopolysaccharide (EPS), a probioactive molecule produced by the same bacterium, showed very significant antioxidant, antihyperglycemic and emulsification activities. Thus, Bacillus coagulans RK - 02 is a well-characterized promising probiotic for its potential commercial applications to pharmaceutical, nutraceutical and functional food formulations with care-free storage.

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Section: Discussionmentioning

confidence: 72%

Molecular Characterization and In Vitro Analyses of a Sporogenous Bacterium with Potential Probiotic Properties

Sen

Pal

Kodali

et al. 2010

Probiotics & Antimicro. Prot.

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“…The intense peak at approximately 2,400 cm −1 in all the systems corresponds to carboxylic acid functional group. These groups in the region of 900−1,400 cm −1 are representatives of polysaccharide part of EPS [34][35][36][37]. In all the three systems, peaks observed in the range of 3,400-3,900 cm −1 might be ascribed to the presence of an adsorbed water molecule and NH/OH groups on SS304 surface [38].…”

Section: Discussionmentioning

confidence: 99%

Role of Bacillus subtilis and Pseudomonas aeruginosa on Corrosion Behaviour of Stainless Steel

et al. 2015

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Corrosion behavior of SS304 in minimal salt medium with 1.5 % NaCl as a corrosive agent in presence of Bacillus subtilis strain S1X and Pseudomonas aeruginosa strain ZK has been investigated. Electrochemical techniques such as Tafel polarization and electrochemical impedance spectroscopy with surface analytical techniques like atomic force microscopy, scanning electron microscopy-energy dispersive spectrum analysis and Fourier transform infrared spectroscopy showed that both bacteria inhibit corrosion of SS304 due to the development of a protective biofilm on metal surface. The pH values of bacterial-inoculated systems decreased with increasing incubation time showing the production of some acidic metabolites by bacterial isolates.

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“…The mechanism of action of these beneficial effects relies on their metabolic end products termed as "probio-active substances" (Naidu et al, 1998). Exopolysaccharides (EPSs) are in the production of fermented dairy products in Northern Europe, Eastern Europe and Asia (Arena et al, 20056;Kodali and Sen, 2009). Polysaccharides derived from diverse microbial genera that include Saccharomyces cerivisiae, Ganoderma applanatum, Cordyceps sinensis have shown very good promise in the treatment of infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

Microbial Exopolysaccharides: Biosynthesis and Potential Applications

Madhuri¹,

Prabhakar²

2014

Orient. J. Chem

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Many bacteria synthesize extracellular polysaccharides (EPSs) with commercially significant physiological and therapeutic activities. Microbial polysaccharides have also been reported to have potential therapeutic applications. Recently, much attention has been devoted to the microbial exopolysaccharides (EPSs) due to their numerous health beneûts.EPSs from lactic acid bacteria are reported to possess antitumor effects, immunostimulatory activity, and the ability to lower blood cholesterol. EPSs also over an alternative class of biothickeners that are widely used in the food and dairy industries and have been proven to provide strong emulsifying activity, which is important in many food formulations. It is also important to understand the mechanism of microbial biosynthesis of EPSs in order to enhance their production by genetic alterations. The potential applications and the mode of microbial biosynthesis of the EPSs have been presented in this article.

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An exopolysaccharide from a probiotic: Biosynthesis dynamics, composition and emulsifying activity

Cited by 84 publications

References 22 publications

Molecular Characterization and In Vitro Analyses of a Sporogenous Bacterium with Potential Probiotic Properties

Molecular Characterization and In Vitro Analyses of a Sporogenous Bacterium with Potential Probiotic Properties

Role of Bacillus subtilis and Pseudomonas aeruginosa on Corrosion Behaviour of Stainless Steel

Microbial Exopolysaccharides: Biosynthesis and Potential Applications

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