An ex vivo evaluation of efficacy of refrigerated canine plasma

Grochowsky, Adam R.; Rozanski, Elizabeth A.; Laforcade, Armelle M. de; Sharp, Claire R.; Meola, Dawn M.; Schavone, Jessica J.; Brooks, Majory B.

doi:10.1111/vec.12202

Cited by 25 publications

(43 citation statements)

References 12 publications

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“…Comparing the mean values of fibrinogen described by Maeckelbergh; Acierno (2008), Geffré et al 2011, Romão et al (2013), andGrochowsky et al (2014), it is verified that there was proximity to those obtained in this study. However, there was a greater dispersion of the values obtained (maximum and minimum values) in the cases of the mentioned authors.…”

Section: Discussionsupporting

confidence: 88%

“…On the other hand, Lopes et al (2005) also used the manual methodology and obtained the mean values of PT closer to those of the present study, yet, with higher values dispersion (4.07 to 9.67 seconds) which suggests a wide variation of results with the use of manual methods. Maeckelbergh;Acierno (2008) and Grochowsky et al (2014) obtained longer prothrombin times in a semiautomatic coagulometer compared to the present study. Similar situation was the comparison with the results described by Romão et al (2013) in citrated plasma of dogs, before the administration of corticosteroids, in which they report a mean value of PT of 11.8 seconds using an automatic methodology.…”

Section: Discussionsupporting

confidence: 55%

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TP, APTT and fibrinogen reference values in healthy beagle breed dogs

Cruz

Martinelli

Brito

et al. 2020

AVB

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 55%

TP, APTT and fibrinogen reference values in healthy beagle breed dogs

Cruz

Martinelli

Brito

et al. 2020

AVB

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“…HSA, an aqueous solution, is seldom linked with bacterial growth. There are only 2 reported incidents of bacterial contamination of human albumin, and both were the result of manufacturer error 11,12 …”

Section: Discussionmentioning

confidence: 99%

The effect of storage at 4°C on canine‐specific albumin on product sterility and albumin concentration

Mastrocco

Cazzolli

Prittie

2021

J Vet Emergen Crit Care

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Objectives To determine if lyophilized canine‐specific albumin (CSA) can be stored beyond the manufacturer recommended 6 hours post‐reconstitution without significant concern for bacterial growth. Design Laboratory research. Setting Large private teaching hospital. Interventions Six bottles of lyophilized CSA were retrieved from a medical grade (4°C) refrigerator and were reconstituted with 0.9% NaCl to a 5% solution. Time to complete dissolution of all bottles at room temperature without agitation was recorded in minutes. A 1.5‐mL sample was collected from each bottle at the following time points: time 0 (at the time of complete dissolution), and then 6, 12, and 24 hours after removal from 4°C. Each aliquot was inoculated into a blood culture tube and was immediately submitted for aerobic and anaerobic bacterial culture. A positive and negative control were cultured at the 24‐hour time point. At each time point, an additional 0.5‐mL aliquot from each albumin bottle was collected, and these were combined to determine the average albumin concentration (g/L, g/dL) as measured by an in‐house analyzer. The albumin was stored at 4°C between sample collection. Measurements and Main Results The reconstitution of CSA and storage at 4°C for up to 24 hours did not result in anaerobic or aerobic bacterial growth in any of the 24 cultured samples. Storage did not have a clinically significant effect on the albumin concentration of the solution. The average albumin concentration of all samples was 42 g/L (4.2 g/dL). No growth was noted in the negative control, and the positive control grew 4 strains of bacteria. Conclusions It appears safe to use lyophilized CSA up to 24 hours post‐reconstitution without significant risk of bacterial growth or change in albumin concentration.

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“…It can be stored for up to 1 year when frozen from −20 to −30 ° C. There is also evidence that thawed FFP can be refrozen within 1 hour of thawing (“freeze‐thaw‐cycled”) and retain its hemostatic protein activity . A recent study found that the use of refrigerated plasma may be feasible with only minor losses in coagulation factor activity . Frozen plasma (FP) is less commonly used in veterinary medicine; it is plasma that is separated from whole blood but not frozen within 8 hours, or that has been frozen quickly but stored between 1 and 4 years.…”

Section: Introductionmentioning

confidence: 99%

Controversies in the use of fresh frozen plasma in critically ill small animal patients

Beer

Silverstein

2015

J Vet Emergen Crit Care

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The use of FFP in critically ill patients remains controversial. In the absence of clinical bleeding or a risk for clinical bleeding associated with a planned procedure, treatment use of FFP is not recommended in human patients. There are insufficient data in critically ill animals to enable formulation of recommendations. Further research is warranted in dogs and cats to establish evidence-based guidelines.

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An ex vivo evaluation of efficacy of refrigerated canine plasma

Abstract: Refrigerated storage results in only minor loss of coagulation factor activity in canine plasma. The use of RP, therefore, may be a viable option in high-volume veterinary hospitals for rapid correction of coagulopathy in critical care patients.

Cited by 25 publications

References 12 publications

TP, APTT and fibrinogen reference values in healthy beagle breed dogs

TP, APTT and fibrinogen reference values in healthy beagle breed dogs

The effect of storage at 4°C on canine‐specific albumin on product sterility and albumin concentration

Controversies in the use of fresh frozen plasma in critically ill small animal patients

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