An evolutionarily conserved motif is required for Plasmodesmata-located protein 5 to regulate cell-to-cell movement

Wang, Xu; Luna, Gabriel Robles; Arighi, Cecilia N.; Lee, Jung Youn

doi:10.1038/s42003-020-1007-0

Cited by 19 publications

(33 citation statements)

References 35 publications

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“…Here, we reported that the transient overexpression of Arabidopsis PDLP5 in N. benthamiana increases the accumulation of callose at PD (Figures 3A,B). The finding is supported by a recent report that the transient overexpression of PDLP5 suppresses the PD-dependent movement of mCherry (Wang et al, 2020). Similar to PDLP5, the transient expression of Arabidopsis PDLP7 also increases callose accumulation at PD in N. benthamiana (Figures 3A,B).…”

Section: Discussionsupporting

confidence: 85%

Plasmodesmata-Dependent Intercellular Movement of Bacterial Effectors

Variz

Chen

et al. 2021

Front. Plant Sci.

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Pathogenic microorganisms deliver protein effectors into host cells to suppress host immune responses. Recent findings reveal that phytopathogens manipulate the function of plant cell-to-cell communication channels known as plasmodesmata (PD) to promote diseases. Several bacterial and filamentous pathogen effectors have been shown to regulate PD in their host cells. A few effectors of filamentous pathogens have been reported to move from the infected cells to neighboring plant cells through PD; however, it is unclear whether bacterial effectors can traffic through PD in plants. In this study, we determined the intercellular movement of Pseudomonas syringae pv. tomato (Pst) DC3000 effectors between adjoining plant cells in Nicotiana benthamiana. We observed that at least 16 Pst DC3000 effectors have the capacity to move from transformed cells to the surrounding plant cells. The movement of the effectors is largely dependent on their molecular weights. The expression of PD regulators, Arabidopsis PD-located protein PDLP5 and PDLP7, leads to PD closure and inhibits the PD-dependent movement of a bacterial effector in N. benthamiana. Similarly, a 22-amino acid peptide of bacterial flagellin (flg22) treatment induces PD closure and suppresses the movement of a bacterial effector in N. benthamiana. Among the mobile effectors, HopAF1 and HopA1 are localized to the plasma membrane (PM) in plant cells. Interestingly, the PM association of HopAF1 does not negatively affect the PD-dependent movement. Together, our findings demonstrate that bacterial effectors are able to move intercellularly through PD in plants.

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Section: Discussionsupporting

confidence: 85%

Plasmodesmata-Dependent Intercellular Movement of Bacterial Effectors

Variz

Chen

et al. 2021

Front. Plant Sci.

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“…Here, we reported that the transient overexpression of Arabidopsis PDLP5 in N. benthamiana increases the accumulation of callose at PD ( Figure 3A-3B). The finding is supported by a recent report that the transient overexpression of PDLP5 suppresses the PD-dependent movement of mCherry (Wang et al, 2020). Similar to PDLP5, the transient expression of Arabidopsis PDLP7 also increases callose accumulation at PD in N. benthamiana ( Figure 3A-3B).…”

Section: Discussionsupporting

confidence: 86%

Plasmodesmata-dependent intercellular movement of bacterial effectors

Variz

Chen

et al. 2020

Preprint

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Pathogenic microorganisms deliver protein effectors into host cells to suppress host immune responses. Recent findings reveal that phytopathogens manipulate the function of plant cell-to-cell communication channels plasmodesmata (PD) to promote diseases. Several bacterial and filamentous pathogen effectors have been shown to regulate PD in their host cells. A few effectors of filamentous pathogens have been reported to move from the infected cells to neighboring plant cells through PD; however, it is unclear whether bacterial effectors can traffic through PD in plants. In this study, we systemically determined the intercellular movement of Pseudomonas syringae pv. tomato (Pst) DC3000 effectors between adjoining plant cells in Nicotiana benthamiana. We observed that at least 16 Pst DC3000 effectors move from transformed cells to the surrounding plant cells. The movement of the effectors is largely dependent on their molecular weights. The expression of PD regulators, Arabidopsis PD-located protein PDLP5 and PDLP7, lead to PD closure and inhibits the PD-dependent movement of a bacterial effector in N. benthamiana. Similarly, a 22-amino acid peptide of bacterial flagellin (flg22) treatment induces PD closure and suppresses the movement of a bacterial effector in N. benthamiana. Together, our findings demonstrated that bacterial effectors are able to move intercellularly through PD in plants.

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“…Certainly, it could be possible that the TMD of those class I formins might have additional functions besides the membrane anchoring. In support of this speculation, a very recent report showed that TMD of Arabidopsis thaliana Plasmodesmata-located protein (PDLP) 5 is involved in the self-interaction of PDLP5 that is essential for PDLP5 to regulate cell-to-cell movement besides its role in membrane targeting ( Wang et al, 2020 ).…”

Section: The Role Of Class I Formins In Regulating the Permeability Omentioning

confidence: 98%

An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins

Diao

Huang

2021

Front. Plant Sci.

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Cell-to-cell communication in plants is mediated by plasmodesmata (PD) whose permeability is tightly regulated during plant growth and development. The actin cytoskeleton has been implicated in regulating the permeability of PD, but the underlying mechanism remains largely unknown. Recent characterization of PD-localized formin proteins has shed light on the role and mechanism of action of actin in regulating PD-mediated intercellular trafficking. In this mini-review article, we will describe the progress in this area.

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An evolutionarily conserved motif is required for Plasmodesmata-located protein 5 to regulate cell-to-cell movement

Cited by 19 publications

References 35 publications

Plasmodesmata-Dependent Intercellular Movement of Bacterial Effectors

Plasmodesmata-Dependent Intercellular Movement of Bacterial Effectors

Plasmodesmata-dependent intercellular movement of bacterial effectors

An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins

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