An evaluation of membrane faecal coliform agar and Colilert-18® for the enumeration of E. coli bacteria in surface water samples

Wohlsen, T.; Bayliss, Julianne; Bates, John; Gray, Bruce; Johnson, Shirley; Schneider, P.

doi:10.2166/aqua.2008.063

Cited by 2 publications

(2 citation statements)

References 11 publications

(26 reference statements)

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“…This has been the case with other enzyme detection methods (e.g. Colilert), when compared with membrane filtration (Wohlsen et al. 2008).…”

Section: Discussionmentioning

confidence: 89%

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Comparative evaluation of chromogenic agar CM1046 and mFC agar for detection of E. coli and thermotolerant coliform bacteria from water samples

Wohlsen¹

2011

Letters in Applied Microbiology

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Aims: The equivalence of Oxoid (CM 1046) Brilliance(TM)E. coli/coliform selective agar to mFC agar, as used in the Australian/New Zealand Standard Method to detect thermotolerant coliforms and Escherichia coli in water samples, was assessed. Methods and Results: A total of 244 water samples were analysed in parallel over a 5‐month period. Sewage effluent samples (n = 131, sites = 43), freshwater (n = 62, sites = 18) and marine/brackish water samples (n = 51, sites = 23) were analysed. The Wilcoxon matched‐pairs signed‐ranks test showed a varying degree of statistical difference between the two methods. All matrices had a higher recovery in the trial method. Enterococci faecalis, Aeromonas spp. and Vibrio spp. did not grow on the CM1046 agar, and Pseudomonas aeruginosa and Enterobacter aerogenes were inhibited. Conclusions: The use of CM 1046 for the detection and enumeration of E. coli and thermotolerant coliforms in water samples is a suitable alternative to the AS/NZS Standard Method. Significance and Impact of the study: The use of CM1046 agar was less labour intensive and time consuming, as no secondary confirmation steps were required. Confirmed results could be reported within 24 h of sample analysis, as compared to 48 h with the reference method. Public health concerns can be addressed in a more efficient manner.

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“…This has been the case with other enzyme detection methods (e.g. Colilert), when compared with membrane filtration (Wohlsen et al. 2008).…”

Section: Discussionmentioning

confidence: 89%

“…The use of fluorogenic and chromogenic substrates for the detection of coliforms and E. coli in both treated and surface waters is gaining in popularity (Hallas et al. 2008; Wohlsen et al. 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of chromogenic agar CM1046 and mFC agar for detection of E. coli and thermotolerant coliform bacteria from water samples

Wohlsen¹

2011

Letters in Applied Microbiology

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Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout

Burnham

Anany

et al. 2014

Anal Bioanal Chem

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Wild-type T4 bacteriophage and recombinant reporter lac Z T4 bacteriophage carrying the β-galactosidase gene were used for detection of generic Escherichia coli by monitoring the release of β-galactosidase upon phage-mediated cell lysis. The reaction was performed on a paper-based portable culture device to limit the diffusion of reagents and, hence, increase the sensitivity of the assay, and to avoid handling large sample volumes, making the assay suitable for on-site analysis. Chromogenic (chlorophenol red-β-D-galactopyranoside, CPRG) and bioluminescent (6-O-β-galactopyranosyl-luciferin, Beta-Glo(®)) β-galactosidase substrates were tested in the assay. Water samples were first filtered through 0.45-μm pore size filters to concentrate bacteria. The filters were then placed into the paper-based device containing nutrient medium and incubated at 37 °C for 4 h. Bacteriophage with the respective indicator substrate was added to the device, and signal (color, luminescence) development was recorded with a digital camera, luminometer, or luminescence imaging device. It was demonstrated that as low as 40 or <10 colony-forming units (cfu) ml(-1) of E. coli can be detected visually within 8 h when wild-type T4 bacteriophage or recombinant lacZ T4 bacteriophage were used in the assay, respectively. Application of the bioluminescent β-galactosidase substrate allowed reliable detection of <10 cfu ml(-1) within 5.5 h. The specificity of the assay was demonstrated using a panel of microorganisms including Aeromonas hydrophila, Enterobacter cloacae, E. coli, and Salmonella Typhimurium.

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An evaluation of membrane faecal coliform agar and Colilert-18® for the enumeration of E. coli bacteria in surface water samples

Cited by 2 publications

References 11 publications

Comparative evaluation of chromogenic agar CM1046 and mFC agar for detection of E. coli and thermotolerant coliform bacteria from water samples

Comparative evaluation of chromogenic agar CM1046 and mFC agar for detection of E. coli and thermotolerant coliform bacteria from water samples

Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout

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