An Evaluation of Endocrine Modulators: Implications for Human Health

Juberg, Daland R.

doi:10.1006/eesa.1999.1851

Cited by 52 publications

(18 citation statements)

References 71 publications

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“…The import of meat from animals treated with zearalanol (zeranol) is banned, while feeding farm animals with naturally Fusarium-infected grain can also lead to the presence of zearalanol residues in pigs and cattle (26,64). In view of increased public awareness of the problem of xenohormones or endocrine disrupters (25) and the need to monitor agricultural products for estrogenic Fusarium mycotoxins, we think that the bioassay which we developed may provide a valuable and economic screening tool. It is difficult to understand why the presence of estrogenic Fusarium mycotoxins in staple food should receive less attention than the presence of zeranol residues in hormone-treated beef or other environmental contaminants, which are orders of magnitude less potent than ZON.…”

Section: Discussionmentioning

confidence: 99%

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

Mitterbauer

Weindorfer

Safaie

et al. 2003

Appl Environ Microbiol

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Zearalenone (ZON) is a nonsteroidal estrogenic mycotoxin produced by plant-pathogenic species of Fusarium. As a consequence of infection with Fusarium culmorum and Fusarium graminearum, ZON can be found in cereals and derived food products. Since ZON is suspected to be a cause of human disease, including premature puberty syndrome, as well as hyperestrogenism in farm animals, several countries have established monitoring programs and guidelines for ZON levels in grain intended for human consumption and animal feed. We developed a low-cost method for monitoring ZON contamination in grain based on a sensitive yeast bioassay. The indicator Saccharomyces cerevisiae strain YZRM7 is unable to grow unless an engineered pyrimidine biosynthetic gene is activated by the expressed human estrogen receptor in the presence of exogenous estrogenic substances. Deletion of the genes encoding ATP-binding cassette (ABC) transporters Pdr5p and Snq2p increases net ZON uptake synergistically. Less than 1 g of ZON per liter of medium is sufficient to allow growth of the indicator strain. To prevent interference with pyrimidines potentially present in biological samples, we also disrupted the genes FUR1 and URK1, blocking the pyrimidine salvage pathway. The bioassay strain YZRM7 allows qualitative detection and quantification of total estrogenic activity in cereal extracts without requiring further cleanup steps. Its high sensitivity makes this assay suitable for low-cost monitoring of contamination of maize and small grain cereals with estrogenic Fusarium mycotxins.

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Section: Discussionmentioning

confidence: 99%

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

Mitterbauer

Weindorfer

Safaie

et al. 2003

Appl Environ Microbiol

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“…Among these compounds are natural and synthetic phytoestrogen and a variety of industrial chemicals (Colburn et al, 1996;Matthiesen et al, 1996). Although the weight of evidence for human effects is minimal according to the WHO agency the International Program for Chemical Safety (IPCS, 2002), and also the EU assessment Scientific Committee on Toxicity, Ecotoxicity and the Environment (SCTEE, 1999), both field and laboratory studies have provided evidence that exposure to these chemicals can lead to the modulation or disruption of development and reproduction in aquatic life and wild life (Kavlock et al, 1996;Tyler et al, 1998;Juberg, 2000;Xu et al, 2002). In order to determine the potential exposure from estrogens in environment samples, chemical analysis is usually essential for determining the concentrations of estrogens.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of estrogenic potencies of estradiol, tamoxifen, bisphenol-A and resveratrol with two in vitro bioassays

Seifert²,

et al. 2004

Environment International

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“…Endocrine disrupters are exogenous compounds thought to mimic the action of estrogen or other hormones and influence endocrine activity in the body (Juberg, 2000). These chemicals have adverse effects not only in the reproductive system but also in the central nervous system during development and throughout life.…”

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confidence: 99%

Effects and Neuro-toxic Mechanisms of 2,2',4,4',5,5'-Hexachlorobiphenyl and Endosulfan in Neuronal Stem Cells.

Kang

Park

Ryu

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Endocrine disrupters are exogenous compounds thought to mimic the action of estrogen or other hormones and influence endocrine activity in the body (Juberg, 2000). These chemicals have adverse effects not only in the reproductive system but also in the central nervous system during development and throughout life. Polychlorinated biphenyls (PCBs) are a class of environmentally persistent and widespread halogenated hydrocarbons. It has been reported that PCBs are potential neurotoxicants. Endosulfan is an organochlorine insecticide that is extensively used to control pests in vegetables, cotton, and fruits. To determine the effect of 2, 2', 4, 4', 5, 5',-hexachlorobiphenyl(2, 4, 5-HCB) and endosulfan on embryo nervous system, we isolated neural stem cells from rat brain at embryonic day 17. Isolated neural stem cells showed pluripotenty. Stem cells could differentiate into neurons and glia. Neurite formation in endosulfan and 2, 4, 5-HCB treated cells. And it appeared to be decreased as compared with that in untreated cells. In order to know the neuro-toxic mechanisms of 2, 4, 5-HCB and endosulfan in neuronal stem cells, we investigated mitogen-activated protein kinase activity (MAPK) and gap junctional intercellular communication (GJIC). Endosulfan decreased the MAPK activity in dose dependent manner. Endosulfan and 2, 4, 5-HCB inhibited GJIC compared to the untreated cell by scrape loading dye transfer (SL/DT). 2, 4, 5-HCB and endosulfan decreased the expression of connexin 43 in dose dependent manner. These results indicated that 2, 4, 5-HCB and endosulfan may inhibit differentiation and proliferation of neural stem cells and gap junctional intercellular communication which play a crucial role in the maintenance of cellular homeostasis. KEY WORDS: endosulfan, gap junctional intercellular communication (GJIC), 2, 2', 4, 4', 5, 5',-hexachlorobiphenyl(2, 4, 5-HCB), mitogenactivated protein kinase (MAPK), neural stem cell.

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An Evaluation of Endocrine Modulators: Implications for Human Health

Cited by 52 publications

References 71 publications

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

Comparative study of estrogenic potencies of estradiol, tamoxifen, bisphenol-A and resveratrol with two in vitro bioassays

Effects and Neuro-toxic Mechanisms of 2,2',4,4',5,5'-Hexachlorobiphenyl and Endosulfan in Neuronal Stem Cells.

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