1993
DOI: 10.1073/pnas.90.15.7119
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: The polyketide-derived macrolactone of the antibiotic erythromycin is made through successive condensation and processing of seven three-carbon units. The fourth cycle involves complete processing of the newly formed 13-keto group (.3keto reduction, dehydration, and enoyl reduction) to yield the methylene that will appear at C-7 of the lactone ring. Synthesis of this molecule in Saccharopolyspora erythraea is determined by the three large eryA genes, organized in six modules, each governing one condensation cy… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

5
101
0
2

Year Published

1996
1996
2017
2017

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 180 publications
(108 citation statements)
references
References 26 publications
5
101
0
2
Order By: Relevance
“…The ChcA, however, catalyzes an enoyl thioester reduction in contrast to the alcohol dehydrogenation catalyzed by other members of the SCAD superfamily (38). It is interesting that the ChcA is not related to a crotonyl CoA reductase recently isolated from S. collinus (62) or any other enoyl thioester reductases involved in either fatty acid biosynthesis (14,30) or secondary metabolism (19,20). The origin of other enzymes in the unusual pathway to cyclohexanecarboxylic acid might provide similar surprises and will be the subject of future investigations.…”
Section: Discussionmentioning
confidence: 99%
“…The ChcA, however, catalyzes an enoyl thioester reduction in contrast to the alcohol dehydrogenation catalyzed by other members of the SCAD superfamily (38). It is interesting that the ChcA is not related to a crotonyl CoA reductase recently isolated from S. collinus (62) or any other enoyl thioester reductases involved in either fatty acid biosynthesis (14,30) or secondary metabolism (19,20). The origin of other enzymes in the unusual pathway to cyclohexanecarboxylic acid might provide similar surprises and will be the subject of future investigations.…”
Section: Discussionmentioning
confidence: 99%
“…As these compounds were isolated from the same microbial culture, it is likely that compound 2 is produced through the same biosynthetic pathway as aldgamycin G, and is then reduced by an enoylreductase. 12 On the basis of the spectroscopic data and biosynthetic considerations, the absolute configuration of 2 is proposed to be identical to that of aldgamycin G (4). The antimicrobial activities of aldgamycin I (1), aldgamycin E (2), aldgamycin F (3), aldgamycin G (4) and chalcomycin (5) were measured using a MIC assay against Micrococcus luteus, Bacillus subtilis, Proteus vulgaris, Salmonella typhimurium, Aspergillus fumigatus, Trichophyton rubrum and methicillin resistant Staphylococcus aureus (Table 3).…”
mentioning
confidence: 99%
“…The first example of such strategy was demonstrated by Donadio et al, where two sites of mutations inactivated the enoyl-reductase (ER) in module 4 for the production of an erythromycin analog. [228] Further trials were put to test by replacing a specific domain or module with its counterpart from different organisms (Figure 14a). [207] This hybrid PKS could successfully adopt the reactions from different organisms into the host PKS machinery.…”
Section: Repurposing Pks For Novel Polyketide Biosynthesismentioning
confidence: 99%