An Enzyme-Linked Immunosorbent Assay Method to Measure Human Apolipoprotein E Levels Using Commercially Available Reagents: Effect of Apolipoprotein E Polymorphism on Serum Apolipoprotein E Concentration

Gràcia, Vicente; Fiol, C.; Hurtado, Isabel; Pintó, Xavier; Argimón, J; Castiñeiras, M J

doi:10.1006/abio.1994.1576

Cited by 19 publications

(7 citation statements)

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“…ApoE knockout mice had increased mesangial cell proliferation and matrix formation compared with wild-type mice or apoB over-expressing mice which have elevated plasma cholesterol and triglycerides [35]. In keeping with the data from neurobiology, apoE may have a role in protecting the kidney from damage by diabetes with the E4 allele being less effective perhaps because the concentration of apoE is less [36]. …”

Section: Discussionmentioning

confidence: 55%

Influence of Apolipoprotein E Genotype on Progression of Diabetic Nephropathy

Joss

Jardine²,

Gaffney³

et al. 2005

Nephron Exp Nephrol

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Aims: Diabetic nephropathy progresses at a variable rate part of which may be explained by genetic polymorphisms. ApoE polymorphisms are associated with progression of atherosclerosis and because of the similarities between atherosclerosis and glomerulosclerosis, we chose to examine apoE and its role in progression of diabetic nephropathy. Methods: The apoE genotypes of 90 patients with type 2 diabetes and nephropathy who were recruited into a 2-year prospective randomised controlled study comparing intensive medical management with routine clinical care were analysed. The primary endpoint was the rate of progression of renal disease in the second year. Results: The apoE genotype frequencies were 49 with E3/3, 20 with E2/3, 17 with E3/4 and 4 with of E2/4. There were no significant differences in age, degree of renal failure, BP, albuminuria or glycaemic control between any genotype. The rate of progression of renal failure of patients with the E3/4 genotype was 0.80 ml/min/month compared to 0.30 ml/min/month for E2/3 and 0.18 ml/min/month for those with E3/3. Patients with E3/4 genotype had significantly faster rates of progression in the second year when compared with the other 3 genotypes (0.80 vs. 0.25 ml/min/month, p = 0.012). There was no difference in mortality according to apoE genotype. Conclusion: Patients who possess the apoE3/4 genotype had significantly faster rates of progression of renal failure.

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Section: Discussionmentioning

confidence: 55%

Influence of Apolipoprotein E Genotype on Progression of Diabetic Nephropathy

Joss

Jardine²,

Gaffney³

et al. 2005

Nephron Exp Nephrol

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“…Human ApoE was measured by sandwich ELISA, using 2E1 monoclonal antibody to ApoE (Boehringer‐Mannheim) to capture and goat anti‐human ApoE antibody (Calbiochem) to detect, with alkaline phosphatase as reporter, as described (Gracia et al, 1994).…”

Section: Human Apoe Elisamentioning

confidence: 84%

Human Apolipoprotein E Isoform‐Specific Differences in Neuronal Sprouting in Organotypic Hippocampal Culture

Teter

Gilbert³

et al. 1999

Journal of Neurochemistry

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Abstract:The apolipoprotein E (ApoE) ⑀4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease. Key Words: Apolipoprotein E-Neurite sprouting-Transgenic mice-Alzheimer's disease.

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“…However, some authors reported higher results [45,46]. For enzyme-linked immunosorbent assay the average values of apoE found by different authors can be divided into two groups: between 2.0 and 4.0 mg/dl [47,48] and between 5.0 and 9.5 mg/dl [49,50]. Electroimmunoassay gives mostly the highest values, ranging from 6.7 to 11.9 mg/dl [51,52].…”

Section: Discussionmentioning

confidence: 99%

Plasma levels of apolipoprotein-E in residents of the European North of Russia

et al. 2013

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BackgroundApolipoprotein-E (apoE) is one of the metabolically active apoproteins and plays an important role in lipid metabolism. However, there are no data on levels of apoE in residents of the North in spite of the fact that specific features of lipid metabolism in the northerners are described. The present work was designed to study plasma levels of apoE in residents of the European North of Russia.MethodsA total of 937 native residents of the European North of Russia (463 men and 474 women) aged 13–60 years were included in the study. ApoE concentrations in the blood plasma were measured by immunoturbidimetric method.ResultsPlasma levels of apoE in residents of the European North of Russia were low. ApoE concentrations below the defined normal values were detected in 57.0% of the men and in 59.2% of the women. The mean plasma levels of apoE did not significantly differ in men and women (2.80 mg/dl vs 2.87 mg/dl). Plasma apoE concentrations in residents of the European North of Russia changed with age. Plasma levels of apoE decreased from 13 to 21 years in men and from 13 to 35 years in women and then increased in both sexes (p < 0.001).ConclusionThe limits of variation of plasma apoE levels in residents of the European North of Russia shift towards lower values. Plasma levels of apoE below normal values were observed in approximately half of investigation subjects.

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An Enzyme-Linked Immunosorbent Assay Method to Measure Human Apolipoprotein E Levels Using Commercially Available Reagents: Effect of Apolipoprotein E Polymorphism on Serum Apolipoprotein E Concentration

Cited by 19 publications

References 0 publications

Influence of Apolipoprotein E Genotype on Progression of Diabetic Nephropathy

Influence of Apolipoprotein E Genotype on Progression of Diabetic Nephropathy

Human Apolipoprotein E Isoform‐Specific Differences in Neuronal Sprouting in Organotypic Hippocampal Culture

Plasma levels of apolipoprotein-E in residents of the European North of Russia

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