An endoplasmic reticulum targeting green fluorescent protein chromophore-based probe for the detection of viscosity

Abstract

The occurrence of endoplasmic reticulum (ER) stress is the main cause of a variety of biological process that are closely related with numerous diseases. The homeostasis of the ER microenvironment...

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

“…[16][17][18][19][20][21] However, there are very few reports accounting for the ER viscosity changes, and analyzing ER viscosity fluctuation during different pathological conditions is barely explored. [22][23][24] Herein, we have developed and synthesized a viscosity sensitive molecular rotor to monitor the ER fate during different biological cell death modes. The derivatives of sulphonylurea are utilized for targeting the ER as this lumen contains sulphonamide receptors.…”

Pentafluorobenzene: Promising Applications in Diagnostics and Therapeutics

“…In a viscous medium, the pentafluorophenyl-induced rotation was restricted. Figure b shows the imaging profile of 36 in MCF-7 cells . In 2022, Tian and co-workers introduced a platinum(II)-5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)porphyrin-based polymerizable oxygen probe 38 (Figure c).…”

“…Scale bar is 15 mm. Reproduced from ref with permission from RSC. (c) Structure of polymer 26 and its oxygen response (refer to the web version of this article to view the legend color).…”

“…Figure 12b shows the imaging profile of 36 in MCF-7 cells. 81 In 2022, Tian and co-workers introduced a platinum(II)-5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)porphyrin-based polymerizable oxygen probe 38 (Figure 12c). Fluoro substituents conjugated with metalloporphyrins assisted in increasing the redox potential, simultaneously improving the photostability and oxidation ability of 38.…”

Construction of a polymer-based fluorescent probe with dual responsive sites for monitoring changes of lysosomal viscosity

“…Fluorescent probes show promise for in situ and real-time detection of target analytes in living systems due to their high sensitivity, noninvasiveness and high site-specificity. [12][13][14][15][16][17][18][19] Several fluorescent probes for monitoring the variation of lysosomal viscosity have been reported. [20][21][22][23][24][25][26] Most of them are designed using hydrophobic aromatic molecules, facing the problem of cytotoxicity.…”