An electrochemical sandwich immunosensor of vascular endothelial growth factor based on reduced graphene oxide/gold nanoparticle composites

Pourmadadi, Mehrab; Shayeh, Javad Shabani; Arjmand, Sareh; Omidi, Meisam; Fatemi, Fataneh

doi:10.1016/j.microc.2020.105476

Cited by 39 publications

(20 citation statements)

References 32 publications

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“…Graphene nanomaterials and metal nanoparticles can increase conductivity, improve specific recognition performance, and heighten biomolecule loading [31, 34, 35]. The applications of graphene oxide and reduced graphene oxide are circumfusing in many realms of science, from food and pharmaceutic industry to environmental and biomedical fields, and biosensing systems, due to the abundance of oxygen functional groups serving as a catalysis for the advancement of electrochemical biosensors, which contributes to boosting aptamer attachment and surface functionalization [31, 36, 37]. Gold nanoparticles (AuNPs) have been extensively used in diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe

Aayanifard

Alebrahim

Pourmadadi

et al. 2021

Engineering in Life Sciences

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The development of a rapid, sensitive, and straightforward detection method of prostate‐specific antigen (PSA) is indispensable for the early diagnosis of prostate cancer (PCa). This work relates an electrochemical method using functionalized single‐stranded DNA aptamer to diagnose PCa and benign prostate hyperplasia. The sensing platform relies on PSA recognition by aptamer/Au/GO‐nanohybrid‐modified glassy carbon electrode. Besides ferrocyanide TiO2/carbon quantum dots (CQDs) probe is used to investigate the effect of nanoparticle‐containing electrolyte. Optimization of incubation time of aptamer/Au/GO‐nanohybrid and volume fraction of nafion were done using Design Expert 10 software reporting 42.4 h and 0.095% V/V, respectively. In ferrocyanide medium, PSA detection as low as 3, 2.96, and 0.85 ng mL−1 was achieved with a dynamic range from 0.5 to 7 ng ml−1, in accord with clinical values, using cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy, respectively. Moreover, this sensor exhibited conspicuous performance in TiO2/CQDs‐containing medium with different pH values of 5.4 and 8 to distinguish total PSA and free PSA, resulting in very low limit of detections, 0.028, and 0.007 ng ml−1, respectively. The results manifested the proposed system as a forthcoming sensor in a clinical and point of care analysis of PSA.

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Section: Introductionmentioning

confidence: 99%

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe

Aayanifard

Alebrahim

Pourmadadi

et al. 2021

Engineering in Life Sciences

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“…Graphene oxide (GO) is a nanomaterial from the graphene family that is obtained by the chemical peeling of graphite using strong oxidizing materials (5). GO can be modified with some other materials such as macromolecules, oxide metals, and metals to improve the electrochemical behavior of the biosensor (6).…”

Section: Introductionmentioning

confidence: 99%

“…Compared to graphene, GO shows different optical, electrical, and electrochemical behavior due to its oxygen-containing structure due to oxidation (8). This carbon nanomaterial has been considered as a promised material in biotechnology (6). FTIR analysis has much information about the structure of GO shows an absorption band at 3360 and 1040 cm −1 correspond to OH and C-O groups, respectively.…”

Section: Introductionmentioning

confidence: 99%

Detection of Microorganisms Using Graphene-Based Nanobiosensors

Pourmadadi

Yazdian

Hojjati

2021

Food Technol. Biotechnol. (Online)

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Having an insight into graphene and graphene derivatives such as graphene oxide, reduced graphene oxide, and graphene quantum dots structures is necessary since it can help scientists to suspect the possible properties and features that using these carbon materials in preparation of a nanocomposite could bring out. In recent years, graphene and its derivatives are attractive with extensive applications in biosensors due to fascinating properties, such as high surface area, optical and magnetic properties, and high elasticity for the detection of microorganisms can be modified with some other materials such as macromolecules, oxide metals, and metals to improve the electrochemical behavior of the biosensor, and also can be modified with some other materials such as macromolecules, oxide metals, and metals to improve the electrochemical behavior of the biosensor. In this review paper, biosensors design strategies based on graphene and its derivatives (graphene-based nanocomposites in biosensors) are introduced. Then their application for the detection of microorganisms including Prions, Viroids, viral and bacterial cells as well as fungi, protozoa, microbial toxins, and even microbial-derived antibiotics are reviewed.

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“…Many reports showed uses of sandwich-type electrochemical immunosensors to detect various kinds of biomarker proteins popularly due to high sensitivity and specificity (Huang et al, 2015 ; Miao et al, 2019 ; Pei et al, 2019a ; Zhang C. et al, 2019 ; Zhang S. et al, 2019 ; Awan et al, 2020 ; Li et al, 2020a ; Pourmadadi et al, 2020 ). Although the immunosensors show high performance in the detection of target analytes, some devices present limits of detection (LOD) comparable to those of label-free configuration, which has less complexity.…”

Section: Introductionmentioning

confidence: 99%

A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

Chanarsa

Jakmunee

2021

Front. Chem.

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A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.

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An electrochemical sandwich immunosensor of vascular endothelial growth factor based on reduced graphene oxide/gold nanoparticle composites

Cited by 39 publications

References 32 publications

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe

Detection of Microorganisms Using Graphene-Based Nanobiosensors

A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

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An electrochemical sandwich immunosensor of vascular endothelial growth factor based on reduced graphene oxide/gold nanoparticle composites

Cited by 39 publications

References 32 publications

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe

Detection of Microorganisms Using Graphene-Based Nanobiosensors

A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

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Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe

Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO₂/carbon quantum dots as a redox probe