An electrochemical immunosensor for testosterone using functionalized magnetic beads and screen-printed carbon electrodes

Eguílaz, Marcos; Moreno‐Guzmán, María; Campuzano, Susana; González‐Cortés, Araceli; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1016/j.bios.2010.07.060

Cited by 132 publications

(49 citation statements)

References 12 publications

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“…They calculated the cross-reactivity (%CR) from EC50 values of individual steroids, and found 10.4 for 19-nortestosterone and 0.8 for β-estradiol. For another steroid hormones exhibited %CR lower than 0.025 (Eguílaz et al, 2010). Coneely et al (2007) found the cross-reactivity (%CR) from EC50 values of 19-nortestosterone and boldenone 11.6% and 9.86%, respectively.…”

Section: Amperometric Biosensors For Hormonesmentioning

confidence: 94%

Electrochemical biosensors for hormone analyses

Bahadır

Sezgintürk

2015

Biosensors and Bioelectronics

140

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Section: Amperometric Biosensors For Hormonesmentioning

confidence: 94%

Electrochemical biosensors for hormone analyses

Bahadır

Sezgintürk

2015

Biosensors and Bioelectronics

140

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“…4), 42 73 testosterone, 74 human growth hormone (hGH), 75 adrenocorticotropin, 76 prolactin, 41 and estradiol. 12 Regarding the field of cardiology, immunosensor devices have been employed for predicting the risk of fatal cardiovascular diseases by sensing the nonspecific biomarker C-reactive protein.…”

Section: Disposable Immusensorsmentioning

confidence: 99%

Disposable Biosensors for Clinical Diagnosis

Janegitz¹,

Cancino²,

Zucolotto³

2014

j. nanosci. nanotech.

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We present an overview on the use of disposable electrochemical biosensors for diagnostics, focusing on the applications of these devices as immunosensors and DNA sensors in the point-of-care diagnostics. Analytical biosensors have emerged as efficient alternatives for the detection of innumerous diseases, because of their high specificity and the convenience of detecting the electrochemical signals produced by the presence of an analyte using a portable equipment. This review highlights the recently developed strategies toward immobilization of different biological molecules on disposable electrodes such as carbon nanotubes and metal nanoparticles. In the course of the review, we first introduced the disposable biosensors, followed by an overview of the immunosensors, and discussed the applications of DNA sensors and disposable biosensors in point-of-care diagnostics. We also have evaluated the prospects and future applications of these devices in the field of biomedical research.

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“…This facilitates the optimization of the experimental conditions and minimizes unspecific bindings on the electrode [22]. This strategy has been used previously to prepare electrochemical immunosensors for the detection of pollutants such as polycyclic hydrocarbons [23], proteins as gliadin [24], microorganisms such as Streptococcus pneumoniae [25], micotoxins [26] and hormones such as testosterone [27] or prolactin [28].…”

Section: Introductionmentioning

confidence: 98%

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

et al. 2012

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In this paper, an electrochemical magneto-immunosensor for the detection of human growth hormone (hGH) is described for the first time. The immunosensor involves the use of tosyl-activated magnetic microparticles (TsMBs) to covalently immobilize a monoclonal mAbhHG antibody. A sandwich-type immunoassay with a secondary pAbhGH antibody and anti-IgG labelled with alkaline phosphatase (anti-IgG-AP) was employed. TsMBs–mAbhGH–hGH–pAbhGH–anti-IgG-AP conjugates were deposited onto the surface of a screen-printed gold electrode using a small neodymium magnet, and electrochemical detection was performed by square-wave voltammetry upon the addition of 4-aminophenyl phosphate as the AP substrate. All the variables involved in the preparation of immunoconjugates and in the immunoassay protocol were optimized. A calibration curve for hGH was constructed with a linear range between 0.01 and 100 ng/mL (r = 0.998) and a limit of detection of 0.005 ng/mL. This value is nearly three orders of magnitude lower than that obtained using surface plasmon resonance (Treviño et al., Talanta 78:1011-1016, 2009). Furthermore, good repeatability, with RSD = 3% (n = 10) at the 1-ng/mL hGH level, was obtained. Cross-reactivity studies with other hormones demonstrated good selectivity. The magneto-immunosensor was applied to the analysis of human serum spiked with hGH at the 4- and 0.1-ng/mL levels. Mean recoveries of 96 ± 6% and 99 ± 2%, respectively, were obtained.

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An electrochemical immunosensor for testosterone using functionalized magnetic beads and screen-printed carbon electrodes

Cited by 132 publications

References 12 publications

Electrochemical biosensors for hormone analyses

Electrochemical biosensors for hormone analyses

Disposable Biosensors for Clinical Diagnosis

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

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