2015
DOI: 10.1007/s10616-014-9810-4 View full text |Buy / Rent full text
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Abstract: Schwann cells (SCs), the supporting cells of the peripheral nerves, are indispensable for regenerating the peripheral and central nervous system. Copious preparation of these cells in a well-defined manner is to be a privileged position. SCs cultivation is overwhelmed by contaminating fibroblasts which are often outgrowing as the predominant cell type in an in vitro culture. This study introduces a technically simple and efficient procedure for SCs isolation and enrichment based on implementing recombinant and… Show more

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“…MTT assay was performed as described elsewhere (Niapour et al 2016a;Sharifi Pasandi et al 2017). Briefly, at the end of the treatment, 20 ll of MTT solution (5 mg/ml in PBS) was added to 180 ll medium in each well and plates were incubated in the dark for 4 h at 37°C.…”
Section: Mtt Assaymentioning
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“…MTT assay was performed as described elsewhere (Niapour et al 2016a;Sharifi Pasandi et al 2017). Briefly, at the end of the treatment, 20 ll of MTT solution (5 mg/ml in PBS) was added to 180 ll medium in each well and plates were incubated in the dark for 4 h at 37°C.…”
Section: Mtt Assaymentioning
“…Owing to the intimate relationship of hDPSCs with the peripheral nervous system, it is not surprising that they can in vitro differentiate to functional Schwann cells. A high proportion of hDPSCs exposed to neural differentiation media co-express p75NTR with S-100β [ 71 ], which constitutes the distinctive molecular marker profile of Schwann cells [ 97 ]. In vitro hDPSC-derived Schwann cells have been shown to help guide axonal extension and myelination [ 98 , 99 ].…”
Section: Results Of Neuronal and Glial Differentiationmentioning
“…Experimentally, embryonic or newborn mice and rats are currently the predominant source of non-myelinating SCs, which have a strong capacity to proliferate ( 23 ). However, in most treatment strategies, SCs are obtained from adult animals.…”
Section: Discussionmentioning