An efficient strategy for the preparation of one-bead-one-peptide libraries on a new biocompatible solid support

Camperi, Silvia A.; Marani, Mariela M.; Iannucci, Nancy B.; Côté, Simon; Alberício, Fernando; Cascone, Osvaldo

doi:10.1016/j.tetlet.2004.12.105

Cited by 43 publications

(39 citation statements)

References 29 publications

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“…This PEG-based matrix allowed peptide synthesis in organic solvents and stability peptide evaluation in aqueous solvents [5]. 4-Hydroxymethylbenzoic acid (HMBA) [6] was used as the linker in order to introduce a cleavage site to release the peptides from the beads before MS analysis.…”

Section: Resultsmentioning

confidence: 99%

Stability Evaluation of Immobilized Peptides Towards Proteases by Mass Spectrometry

Giudicessi¹,

Salum²,

Martínez‐Ceron³

et al. 2015

Peptides 2015, Proceedings of the 24th American Peptide Symposium

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Section: Resultsmentioning

confidence: 99%

Stability Evaluation of Immobilized Peptides Towards Proteases by Mass Spectrometry

Giudicessi¹,

Salum²,

Martínez‐Ceron³

et al. 2015

Peptides 2015, Proceedings of the 24th American Peptide Symposium

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“…These supports have good swelling properties in both polar and apolar solvents, facilitating the preparation of highly structured peptides [7] [8]. Moreover, peptide libraries have been prepared by using apolar solvents, and their binding properties have been analyzed on the same supports using aqueous solvents [6].…”

Section: Synthesis Of Oligonucleotide Derivatives Using Chemmatrix Sumentioning

confidence: 99%

Synthesis of Oligonucleotide Derivatives Using ChemMatrix Supports

Mazzini

García-Martín

Alvira

et al. 2008

Chemistry & Biodiversity

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The synthesis of oligonucleotides on poly(ethylene glycol)-based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthesis of oligonucleotide-peptide conjugates and for the ligation of oligonucleotides using Cu(+)-catalyzed cycloadition reactions is reported. Moreover, these supports can be used for the preparation of oligonucleotides in anhydrous solvents, followed by hybridization of the complementary sequences in aqueous buffers.

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“…The bond formed between the first amino acid and the linker HMBA is stable to all synthetic elongation reactions as well as to the conditions required for the removal of the side-chain protecting group. The solid support is amenable to easy release of the peptide for mass spectrometry (MS) analysis [5,6]. For peptides shorter than six amino acids, Ala and/or Gly residues may be introduced in the C-termini to increase peptide molecular weight in order to facilitate MS analysis.…”

Section: Introductionmentioning

confidence: 99%

Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

Camperi

Martínez‐Ceron

Giudicessi

et al. 2014

Methods in Molecular Biology

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We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: (a) synthesis of a combinatorial library on the hydromethylbenzoyl (HMBA)-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry, (b) library screening with the protein of interest labeled with a fluorescent dye or biotin, (c) identification of peptides contained on positive beads by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), (d) solid-phase peptide ligand synthesis and immobilization in chromatographic supports, and (e) evaluation of protein adsorption on peptide affinity matrices from the equilibrium isotherms and breakthrough curves.

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An efficient strategy for the preparation of one-bead-one-peptide libraries on a new biocompatible solid support

Cited by 43 publications

References 29 publications

Stability Evaluation of Immobilized Peptides Towards Proteases by Mass Spectrometry

Stability Evaluation of Immobilized Peptides Towards Proteases by Mass Spectrometry

Synthesis of Oligonucleotide Derivatives Using ChemMatrix Supports

Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

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