An efficient strategy for the extraction and purification of lignans fromSchisandra chinensisby a combination of supercritical fluid extraction and high-speed counter-current chromatography

Abstract

An efficient strategy for extracting and separating five lignans from Schisandra chinensis (Turcz.) Baill has been developed using supercritical fluid extraction (SFE) and high-speed counter-current chromatography (HSCCC) in the present study. First, the extraction was performed by a preparative SFE system under 15 MPa of pressure at 36°C for 4 h. Then, the SFE extract was successfully separated and purified by HSCCC with a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (6:4:5:5, 6:4:6:4, 6:4:8:2, v/v) in a stepwise elution mode. The fractions were analyzed by HPLC, and the chemical structures of the products were identified by ESI-MS and 1H NMR spectroscopy. As a result, a total of 12.5 mg of schisandrin at 98.0% purity, 7.1 mg of gomisin A at 98.1% purity, 1.8 mg of schisantherin B at 93.3% purity, 4.4 mg of deoxyschisandrin at 92.9% purity, and 6.8 mg of γ-schisandrin at 89.1% purity were obtained from 300 mg crude extract in a one-step purification.

Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies

“…Among others we can mention counter current chromatography (Chu et al 2013; Li et al 2014; Zhu et al 2014b, 2015), extraction with supercritical CO 2 (Zhu et al 2014a) extraction with ultrasounds (Zhang et al 2014c), extraction with microwaves (Ma et al 2012b) and application of supercritical antisolvent precipitation (Huang et al 2013). …”

Novel immunoassay and rapid immunoaffinity chromatography method for the detection and selective extraction of naringin in Citrus aurantium

“…Generally, strategies for the separation and purification of natural products have been based on chromatographic techniques such as column chromatography on silica gel, Rp‐18, and Sephadex LH‐20 . Recently, some strategies using preparative HPLC and high‐speed countercurrent chromatography have been reported. To obtain a sufficient amount of high‐purity compound for structural and pharmacological analyses, tedious procedures, including large amounts of extraction solvents, repeated cycles of extraction, fractionation, and purification steps, are necessary.…”

Isolation and purification of two antioxidant isomers of resveratrol dimer from the wine grape by counter‐current chromatography

“…CCC is a support‐free liquid–liquid partition chromatography without the use of a solid support matrix . Recently, the method has been successfully applied to separate and analyze the various polyphenols from plant extracts []. However, there has been no report about the use of CCC for the isolation and purification of isomer of resveratrol dimer from wine grape.…”