An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags

Cheng, Siyun; Tu, Zhijie; Zheng, Shuai; Cheng, Xiaodan; Han, Han; Wang, Chongwen; Xiao, Rui; Gu, Bing

doi:10.1016/j.aca.2021.339155

Cited by 46 publications

(14 citation statements)

References 51 publications

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“…The dissolved solution was poured into a high-pressure reactor and then heated at 210 °C for 12 h. The reaction products were washed with ethanol and ultra-pure water followed by drying in a vacuum at 60 °C for about 6 hours. 11 , 21 …”

Section: Methodsmentioning

confidence: 99%

A SERS Platform for Rapid Detection of Drug Resistance of Non-Candida albicans Using Fe3O4@PEI and Triangular Silver Nanoplates

Gu¹,

Hu²,

Wu³

et al. 2022

IJN

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Purpose Candida infection has a high mortality rate, and the increasing prevalence of non- Candida albicans drug resistance in recent years poses a potential threat to human health. Non- Candida albicans has long culture cycles, and its firm cell walls making it difficult to isolate DNA for sequencing. Materials and Methods Fe 3 O 4 @PEI (PEI, polyvinyl imine) was mixed with clinical samples to form Fe 3 O 4 @PEI@non- Candida albicans and enriched them with magnets. Triangular silver nanoplates enhanced the surface-enhanced Raman scattering (SERS) signal. SERS was used to detect the fingerprint spectrum of non-Candida albicans . Then, orthogonal partial least squares discriminant analysis (OPLS-DA) was used to analyze the drug resistance of non- Candida albicans. Results SERS combined with OPLS-DA could well analyze the drug resistance of non- Candida albicans. Through 10-fold-cross validation, the accuracy of training and test data is greater than 99%, indicating that the model has good classification ability. We used SERS for the first time to detect the drug resistance of non- Candida albicans directly. Conclusion This approach can be utilized without causing damage to the cell wall and can be accomplished in as little as 90 minutes. It can provide timely guidance for the treatment of patients with good clinical application potential.

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Section: Methodsmentioning

confidence: 99%

A SERS Platform for Rapid Detection of Drug Resistance of Non-Candida albicans Using Fe3O4@PEI and Triangular Silver Nanoplates

Gu¹,

Hu²,

Wu³

et al. 2022

IJN

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“…Cheng et al. (2021) described a strategy for the separation of S. aureus and L. monocytogenes based on broad‐spectrum wheat germ agglutinin‐modified MNPs, and the results showed that the functionalized‐MNPs exhibited high bacterial capture efficiency (73.2%−94.2%) for both target bacteria at low concentrations (10−10 4 cells/ml) (Cheng et al., 2021).…”

Section: Biorecognition Reagents Modified On Mnpsmentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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Foodborne pathogens contamination is one of the main sources of food safety problems. Although the existing detection methods have been developed for a long time, the complexity of food samples is still the main factor affecting the detection time and sensitivity, and the rapid separation and enrichment of pathogens is still an objective to be studied. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered to be an effective tool for rapid separation and enrichment of foodborne pathogens in food. Therefore, this study comprehensively reviews the development of MNPs in the separation of foodborne pathogens over the past decade. First, various biorecognition reagents for identification of foodborne pathogens and their modifications on the surface of MNPs are introduced. Then, the factors affecting the separation of foodborne pathogens, including the size of MNPs, modification methods, separation strategies and separation forms are discussed. Finally, the application of MNPs in integrated detection methods is reviewed. Moreover, current challenges and prospects of MNPs for the analysis of foodborne pathogens are discussed. Further research should focus on the design of multifunctional MNPs, the processing of large‐scale samples, the simultaneous analysis of multiple targets, and the development of all‐in‐one small analytical device with separation and detection.

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“…For example, wheat germ agglutinin (WGA) can specically recognize N-acetylglucosamine on the cell wall of most foodborne pathogens. 23,24 Compared with other biorecognition molecules such as antibodies and aptamers, WGA not only have the advantages of good stability and low cost but also has broad-spectrum bacteria binding ability. However, there are still no reports about the introduction of lectin into LFA method for bacteria detection.…”

Section: Introductionmentioning

confidence: 99%

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags

Cheng

Dong

et al. 2022

RSC Adv.

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An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags

Cited by 46 publications

References 51 publications

A SERS Platform for Rapid Detection of Drug Resistance of Non-Candida albicans Using Fe3O4@PEI and Triangular Silver Nanoplates

A SERS Platform for Rapid Detection of Drug Resistance of Non-Candida albicans Using Fe3O4@PEI and Triangular Silver Nanoplates

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags

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