An efficient protocol for DNA extraction from Meliolales and the description of Meliola centellae sp. nov.

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Currently there is a large and increasing demand for immature coconuts for fresh consumption of their liquid endosperm (coconut water). The occurrence of post-harvest diseases prevent its sale and change the taste, making it inappropriate for consumption. In 2011, coconut fruits showing internal post-harvest rot symptoms were found in a market in Belo Horizonte and after incubation for 5 days in a humid chamber at room temperature, fungal structures were observed. Fruiting structures show morphology typical of Ceratocystis paradoxa. For molecular identification, DNA sequences were generated for the Internal Transcribed Spacer regions 1 and 2 including the 5.8S rRNA gene, part of the β-tubulin and the Transcription Elongation Factor 1-α gene regions. These data were compared with those of other C. paradoxa using phylogenetic analysis. Koch's postulates was confirmed by inoculation of 6-mm-diameter PDA plugs with the isolate on fruits of coconut. Morphology of the isolates in culture as well as phylogenetic inference showed that the causal agent of internal post-harvest rot disease on coconut is C. paradoxa.

mentioning

confidence: 99%

Notes on Ceratocystis paradoxa causing internal post-harvest rot disease on immature coconut in Brazil

Pinho¹,

Dutra²,

Pereira³

2013

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“…The crushing was resumed after adding 100 µL of Nuclei Lysis Solution of the Wizard Genomic DNA Purification kit (Promega). Extraction continued as described by Pinho et al (2012). PCR reagents, primers and conditions were as described by Machado et al (2014).…”

mentioning

confidence: 99%

New occurrences of Botryosphaeriaceae causing black root rot of cassava in Brazil

Machado¹,

Pinho²,

Oliveira³

et al. 2014

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Despite the occurrence of several diseases of cassava, the cassava black root rot (CBR) represents one of the main limiting factor for crop rentability in the world. However, the etiology of CBR is complex and it needs to be revised based on current molecular analysis. On this work, molecular and morphological studies allowed for the identification of three species of Botryosphaeriaceae causing black root rot disease of cassava in the states of Maranhão and Paraíba, Brazil, namely: Lasiodiplodia euphorbicola, Lasiodiplodia pseudotheobromae and Neoscytalidium hyalinum. This is the first report of these three fungal species as causal agents of CBR in the world.

“…The genomic material was extracted from approximately 40 mg of fungal tissue using the Wizard Genomic DNA Purification Kit (Promega) following the protocol described by Pinho et al (2012).…”

mentioning

confidence: 99%

“…The phylogenetic analysis of the concatenated alignment was performed on the CIPRES web portal (Miller et al, 2010) using MrBayes v.3.1.1 (Ronquist & Huelsenbeck, 2003). The remainder of the phylogenetic analysis was conducted as described by Pinho et al (2012).…”

mentioning

confidence: 99%

Postharvest rot and mummification of strawberry fruits caused by Neofusicoccum parvum and N. kwambonambiense in Brazil

Lopes

Zambolim

Pinho

et al. 2014

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In addition to the rots that are commonly found on strawberries, a new disease was found in 7% of stored fruits during a survey of strawberry diseases at the postharvest stage. Koch's postulates were satisfied, and the fungi were identified as Neofusicoccum kwambonambiense and N. parvum based on morphology and phylogenetic analysis of the internal transcribed spacers, β-tubulin, RNA polymerase subunit II and transcription elongation factor 1-α regions. This is the first report of Neofusicoccum kwambonambiense in Brazil and the first report of Neofusicoccum spp. causing mummification and postharvest rot of strawberry.